Psychrobacter pygoscelis sp. nov. isolated from the penguin pygoscelis papua

• Published in: International Journal of Systematic and Evolutionary Microbiology
• Main Authors: Kämpfer, P., Glaeser, S.P., Irgang, R., Fernández-Negrete, G., Poblete-Morales, M., Fuentes-Messina, D., Cortez-San Martín, M., Avendaño-Herrera, R.
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2020
• Subjects: 579; Antarc*; Antarctic; Gentoo penguin; Pygoscelis papua
• Online Access: https://publica.fraunhofer.de/handle/publica/263054; https://doi.org/10.1099/ijsem.0.003739

S.211-219 One slightly beige-white pigmented, Gram-stain-negative, rod-shaped bacterium, strain I-STPP5bT, was isolated from the trachea of a Gentoo penguin chick individual (Pygoscelin papua) investigated in Fildes Bay, Chilean Antarctic (62° 12' S, 58° 57' W). I-STPP5bT consists of a 3.4 Mb chromosome with a DNA G+C content of 44.4 mol%. Of the 3056 predicted genes, 1206 were annotated as hypothetical proteins and 51 were tRNAs. Phylogenetic analysis based on nearly full-length 16S rRNA gene sequences showed that the isolate shared a 16S rRNA gene sequence identity to the type strains of Psychrobacter phenylpyruvicus (98.8 %), Psychrobacter arenosus and Psychrobacter pasteurii (both 98.3 %), Psychrobacter piechaudii (98.2 %) and Psychrobacter sanguinis (98.1 %), but 16S rRNA gene sequence similarities to all other Psychrobacter species were <98.0 %. Partial gyrB nucleotide and amino acid sequence similarities among strain STPP5bT and the next related type strains were all below 81.8 and 92.9%, respectively. DNA-DNA hybridisation (DDH) with P. phenylpyruvicus LMG 5372T, P. arenosus DSM 15389T and P. sanguinis DSM 23635T also showed low values (all below 30 %). The main cellular fatty acids of the strain were C18 : 1o9c and C16 : 1o7c and/or C16 : 1o6c. Based on phylogenetic, chemotaxonomic, genomic and phenotypic analyses we propose a new species of the genus Psychrobacter, with the name Psychrobacter pygoscelis sp. nov. and strain I-STPP5bT (=CIP 111410T= CCM 8799T=LMG 30301T) as type strain. 70 Nr.1