Alginate overproduction and biofilm formation by psychrotolerant Pseudomonas mandelii depend on temperature in Antarctic marine sediments
S.27-34 Background: In recent years, Antarctica has become a key source of biotechnological resources. Native microorganisms have developed a wide range of survival strategies to adapt to the harsh Antarctic environment, including the formation of biofilms. Alginate is the principal component of the...
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ftfrauneprints:oai:publica.fraunhofer.de:publica/251589 2024-05-12T07:55:52+00:00 Alginate overproduction and biofilm formation by psychrotolerant Pseudomonas mandelii depend on temperature in Antarctic marine sediments Vasquez-Ponce, F. Higuera-Llanten, S. Pavlov, M.S. Ramirez-Orellana, R. Marshall, S.H. Olivares-Pacheco, J. 2017 https://publica.fraunhofer.de/handle/publica/251589 https://doi.org/10.1016/j.ejbt.2017.05.001 en eng Electronic journal of biotechnology : EJB doi:10.1016/j.ejbt.2017.05.001 https://publica.fraunhofer.de/handle/publica/251589 journal article 2017 ftfrauneprints https://doi.org/10.1016/j.ejbt.2017.05.001 2024-04-17T14:34:30Z S.27-34 Background: In recent years, Antarctica has become a key source of biotechnological resources. Native microorganisms have developed a wide range of survival strategies to adapt to the harsh Antarctic environment, including the formation of biofilms. Alginate is the principal component of the exopolysaccharide matrix in biofilms produced by Pseudomonas, and this component is highly demanded for the production of a wide variety of commercial products. There is a constant search for efficient alginate-producing organisms. Results: In this study, a novel strain of Pseudomonas mandelii isolated from Antarctica was characterized and found to overproduce alginate compared with other good alginate producers such as Pseudomonas aeruginosa and Pseudomonas fluorescens. Alginate production and expression levels of the alginate operon were highest at 4 degrees C. It is probable that this alginate-overproducing phenotype was the result of downregulated MucA, an anti-sigma factor of AlgU. Conclusion: Because biofilm formation is an efficient bacterial strategy to overcome stressful conditions, alginate overproduction might represent the best solution for the successful adaptation of P. mandelii to the extreme temperatures of the Antarctic. Through additional research, it is possible that this novel P. mandelii strain could become an additional source for biotechnological alginate production. 28 Article in Journal/Newspaper Antarc* Antarctic Antarctica Publikationsdatenbank der Fraunhofer-Gesellschaft Antarctic The Antarctic Electronic Journal of Biotechnology 28 27 34 |
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Publikationsdatenbank der Fraunhofer-Gesellschaft |
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ftfrauneprints |
language |
English |
description |
S.27-34 Background: In recent years, Antarctica has become a key source of biotechnological resources. Native microorganisms have developed a wide range of survival strategies to adapt to the harsh Antarctic environment, including the formation of biofilms. Alginate is the principal component of the exopolysaccharide matrix in biofilms produced by Pseudomonas, and this component is highly demanded for the production of a wide variety of commercial products. There is a constant search for efficient alginate-producing organisms. Results: In this study, a novel strain of Pseudomonas mandelii isolated from Antarctica was characterized and found to overproduce alginate compared with other good alginate producers such as Pseudomonas aeruginosa and Pseudomonas fluorescens. Alginate production and expression levels of the alginate operon were highest at 4 degrees C. It is probable that this alginate-overproducing phenotype was the result of downregulated MucA, an anti-sigma factor of AlgU. Conclusion: Because biofilm formation is an efficient bacterial strategy to overcome stressful conditions, alginate overproduction might represent the best solution for the successful adaptation of P. mandelii to the extreme temperatures of the Antarctic. Through additional research, it is possible that this novel P. mandelii strain could become an additional source for biotechnological alginate production. 28 |
format |
Article in Journal/Newspaper |
author |
Vasquez-Ponce, F. Higuera-Llanten, S. Pavlov, M.S. Ramirez-Orellana, R. Marshall, S.H. Olivares-Pacheco, J. |
spellingShingle |
Vasquez-Ponce, F. Higuera-Llanten, S. Pavlov, M.S. Ramirez-Orellana, R. Marshall, S.H. Olivares-Pacheco, J. Alginate overproduction and biofilm formation by psychrotolerant Pseudomonas mandelii depend on temperature in Antarctic marine sediments |
author_facet |
Vasquez-Ponce, F. Higuera-Llanten, S. Pavlov, M.S. Ramirez-Orellana, R. Marshall, S.H. Olivares-Pacheco, J. |
author_sort |
Vasquez-Ponce, F. |
title |
Alginate overproduction and biofilm formation by psychrotolerant Pseudomonas mandelii depend on temperature in Antarctic marine sediments |
title_short |
Alginate overproduction and biofilm formation by psychrotolerant Pseudomonas mandelii depend on temperature in Antarctic marine sediments |
title_full |
Alginate overproduction and biofilm formation by psychrotolerant Pseudomonas mandelii depend on temperature in Antarctic marine sediments |
title_fullStr |
Alginate overproduction and biofilm formation by psychrotolerant Pseudomonas mandelii depend on temperature in Antarctic marine sediments |
title_full_unstemmed |
Alginate overproduction and biofilm formation by psychrotolerant Pseudomonas mandelii depend on temperature in Antarctic marine sediments |
title_sort |
alginate overproduction and biofilm formation by psychrotolerant pseudomonas mandelii depend on temperature in antarctic marine sediments |
publishDate |
2017 |
url |
https://publica.fraunhofer.de/handle/publica/251589 https://doi.org/10.1016/j.ejbt.2017.05.001 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_relation |
Electronic journal of biotechnology : EJB doi:10.1016/j.ejbt.2017.05.001 https://publica.fraunhofer.de/handle/publica/251589 |
op_doi |
https://doi.org/10.1016/j.ejbt.2017.05.001 |
container_title |
Electronic Journal of Biotechnology |
container_volume |
28 |
container_start_page |
27 |
op_container_end_page |
34 |
_version_ |
1798835747035283456 |