Long-term culture of a cell population from Siberian sturgeon (Acipenser baerii) head kidney
S.367-372 In vitro cultures of native fish cell lines are of great importance, both for basic research and applied science. In particular, there is strong demand for long-term growable cell lines from breeding fish, like sturgeon. Here, we describe the culture of cells from Siberian sturgeon (Acipen...
Published in: | Fish Physiology and Biochemistry |
---|---|
Main Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2008
|
Subjects: | |
Online Access: | https://publica.fraunhofer.de/handle/publica/216814 https://doi.org/10.1007/s10695-007-9196-8 |
id |
ftfrauneprints:oai:publica.fraunhofer.de:publica/216814 |
---|---|
record_format |
openpolar |
spelling |
ftfrauneprints:oai:publica.fraunhofer.de:publica/216814 2024-05-19T07:27:22+00:00 Long-term culture of a cell population from Siberian sturgeon (Acipenser baerii) head kidney Ciba, P. Schicktanz, S. Anders, E. Siegl, E. Stielow, A. Klink, E. Kruse, C. 2008 https://publica.fraunhofer.de/handle/publica/216814 https://doi.org/10.1007/s10695-007-9196-8 en eng Fish physiology and biochemistry doi:10.1007/s10695-007-9196-8 https://publica.fraunhofer.de/handle/publica/216814 597 journal article 2008 ftfrauneprints https://doi.org/10.1007/s10695-007-9196-8 2024-04-30T23:56:02Z S.367-372 In vitro cultures of native fish cell lines are of great importance, both for basic research and applied science. In particular, there is strong demand for long-term growable cell lines from breeding fish, like sturgeon. Here, we describe the culture of cells from Siberian sturgeon (Acipenser baerii) head kidney. The cells have so far been cultured over a period of 12 months (24 passages). Cytochemical and immunocytochemical examination suggests that, in vitro, the cells exhibit markers that are indicative for different cell types. In particular, fat storing cells (adipocytes) were observed, and the expression of cytokeratins and glial fibrilar acidic protein (GFAP) can be concluded on the basis of immuncytochemical analysis. The observation of different morphologies additionally underlines the heterogeneity of the cell population and matches the typical behaviour of in vitro cultures of stem/progenitor cells. Different applications can be imagined. 34 Nr.4 Article in Journal/Newspaper Acipenser baerii Siberian sturgeon Publikationsdatenbank der Fraunhofer-Gesellschaft Fish Physiology and Biochemistry 34 4 367 372 |
institution |
Open Polar |
collection |
Publikationsdatenbank der Fraunhofer-Gesellschaft |
op_collection_id |
ftfrauneprints |
language |
English |
topic |
597 |
spellingShingle |
597 Ciba, P. Schicktanz, S. Anders, E. Siegl, E. Stielow, A. Klink, E. Kruse, C. Long-term culture of a cell population from Siberian sturgeon (Acipenser baerii) head kidney |
topic_facet |
597 |
description |
S.367-372 In vitro cultures of native fish cell lines are of great importance, both for basic research and applied science. In particular, there is strong demand for long-term growable cell lines from breeding fish, like sturgeon. Here, we describe the culture of cells from Siberian sturgeon (Acipenser baerii) head kidney. The cells have so far been cultured over a period of 12 months (24 passages). Cytochemical and immunocytochemical examination suggests that, in vitro, the cells exhibit markers that are indicative for different cell types. In particular, fat storing cells (adipocytes) were observed, and the expression of cytokeratins and glial fibrilar acidic protein (GFAP) can be concluded on the basis of immuncytochemical analysis. The observation of different morphologies additionally underlines the heterogeneity of the cell population and matches the typical behaviour of in vitro cultures of stem/progenitor cells. Different applications can be imagined. 34 Nr.4 |
format |
Article in Journal/Newspaper |
author |
Ciba, P. Schicktanz, S. Anders, E. Siegl, E. Stielow, A. Klink, E. Kruse, C. |
author_facet |
Ciba, P. Schicktanz, S. Anders, E. Siegl, E. Stielow, A. Klink, E. Kruse, C. |
author_sort |
Ciba, P. |
title |
Long-term culture of a cell population from Siberian sturgeon (Acipenser baerii) head kidney |
title_short |
Long-term culture of a cell population from Siberian sturgeon (Acipenser baerii) head kidney |
title_full |
Long-term culture of a cell population from Siberian sturgeon (Acipenser baerii) head kidney |
title_fullStr |
Long-term culture of a cell population from Siberian sturgeon (Acipenser baerii) head kidney |
title_full_unstemmed |
Long-term culture of a cell population from Siberian sturgeon (Acipenser baerii) head kidney |
title_sort |
long-term culture of a cell population from siberian sturgeon (acipenser baerii) head kidney |
publishDate |
2008 |
url |
https://publica.fraunhofer.de/handle/publica/216814 https://doi.org/10.1007/s10695-007-9196-8 |
genre |
Acipenser baerii Siberian sturgeon |
genre_facet |
Acipenser baerii Siberian sturgeon |
op_relation |
Fish physiology and biochemistry doi:10.1007/s10695-007-9196-8 https://publica.fraunhofer.de/handle/publica/216814 |
op_doi |
https://doi.org/10.1007/s10695-007-9196-8 |
container_title |
Fish Physiology and Biochemistry |
container_volume |
34 |
container_issue |
4 |
container_start_page |
367 |
op_container_end_page |
372 |
_version_ |
1799471775142117376 |