Use of Cellulose Acetate Electrophoresis in the Taxonomy of Steinernematids (Rhabditida, Nematoda)
A steinernematid nematode was isolated from soil samples collected near St. John's, Newfoundland, Canada. On the basis of its morphometry and RFLPs in ribosomal DNA spacer, it was designated as a new strain, NF, of Steinernemafeltiae. Cellulose acetate electrophoresis was used to separate isozy...
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Journal of Nematology
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ftfloridaojojs:oai:journals.flvc.org:article/66821 2023-05-15T17:21:50+02:00 Use of Cellulose Acetate Electrophoresis in the Taxonomy of Steinernematids (Rhabditida, Nematoda) Jagdale, G. B. Gordon, Roger Vrain, T. C. 1996-09-15 application/pdf https://journals.flvc.org/jon/article/view/66821 eng eng Journal of Nematology https://journals.flvc.org/jon/article/view/66821/64489 https://journals.flvc.org/jon/article/view/66821 Journal of Nematology; Vol. 28, No. 3 (September 1996); 301-309 0022-300X info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 1996 ftfloridaojojs 2020-11-14T18:32:04Z A steinernematid nematode was isolated from soil samples collected near St. John's, Newfoundland, Canada. On the basis of its morphometry and RFLPs in ribosomal DNA spacer, it was designated as a new strain, NF, of Steinernemafeltiae. Cellulose acetate electrophoresis was used to separate isozymes of eight enzymes in infective juveniles of S. feltiae NF as well as four other isolates: S. feltiae Umeå strain, S. feltiae L1C strain, Steinernema carpocapsae All strain, and Steinernema riobravis TX strain. Based on comparisons of the relative electrophoretic mobilities ([mu]) of the isozymes, one of the eight enzymes (arginine kinase) yielded zymograms that were distinctive for each of the isolates, except for the Umeå and NF strains of S. feltiae, which had identical banding patterns. Four enzymes (fumarate hydratase, phosphoglucoisomerase, phosphoglucomutase, and 6-phosphogluconate dehydrogenase) yielded isozyme banding patterns that were characteristic for all isolates, except for the L1C and NF strains of S. feltiae, which were identical. Two enzymes (aspartate amino transferase and glycerol-3-phosphate dehydrogenase) yielded zymograms that permitted S. carpocapsae All strain to be discriminated from the other four isolates, while the remaining enzyme (mannose-6-phosphate isomerase) was discriminatory for S. riobravis TX strain. Except for one enzyme, the isozyme banding pattern of the NF isolate of S. feltiae was the same as in the L1C strain, isolated 13 years previously from Newfoundland. Cellulose acetate electrophoresis could prove invaluable for taxonomic identification of isolates of steinernematids, provided that a combination of enzymes is used. Key words: cellulose acetate electrophoresis, entomopathogenic nematode, isozyme, ribosomal DNA, Steinernema carpocapsae, Steinernema feltiae, Steinernema riobravis, steinernematid, taxonomy. Article in Journal/Newspaper Newfoundland Florida Online Journals (FloridaOJ) Canada |
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Florida Online Journals (FloridaOJ) |
op_collection_id |
ftfloridaojojs |
language |
English |
description |
A steinernematid nematode was isolated from soil samples collected near St. John's, Newfoundland, Canada. On the basis of its morphometry and RFLPs in ribosomal DNA spacer, it was designated as a new strain, NF, of Steinernemafeltiae. Cellulose acetate electrophoresis was used to separate isozymes of eight enzymes in infective juveniles of S. feltiae NF as well as four other isolates: S. feltiae Umeå strain, S. feltiae L1C strain, Steinernema carpocapsae All strain, and Steinernema riobravis TX strain. Based on comparisons of the relative electrophoretic mobilities ([mu]) of the isozymes, one of the eight enzymes (arginine kinase) yielded zymograms that were distinctive for each of the isolates, except for the Umeå and NF strains of S. feltiae, which had identical banding patterns. Four enzymes (fumarate hydratase, phosphoglucoisomerase, phosphoglucomutase, and 6-phosphogluconate dehydrogenase) yielded isozyme banding patterns that were characteristic for all isolates, except for the L1C and NF strains of S. feltiae, which were identical. Two enzymes (aspartate amino transferase and glycerol-3-phosphate dehydrogenase) yielded zymograms that permitted S. carpocapsae All strain to be discriminated from the other four isolates, while the remaining enzyme (mannose-6-phosphate isomerase) was discriminatory for S. riobravis TX strain. Except for one enzyme, the isozyme banding pattern of the NF isolate of S. feltiae was the same as in the L1C strain, isolated 13 years previously from Newfoundland. Cellulose acetate electrophoresis could prove invaluable for taxonomic identification of isolates of steinernematids, provided that a combination of enzymes is used. Key words: cellulose acetate electrophoresis, entomopathogenic nematode, isozyme, ribosomal DNA, Steinernema carpocapsae, Steinernema feltiae, Steinernema riobravis, steinernematid, taxonomy. |
format |
Article in Journal/Newspaper |
author |
Jagdale, G. B. Gordon, Roger Vrain, T. C. |
spellingShingle |
Jagdale, G. B. Gordon, Roger Vrain, T. C. Use of Cellulose Acetate Electrophoresis in the Taxonomy of Steinernematids (Rhabditida, Nematoda) |
author_facet |
Jagdale, G. B. Gordon, Roger Vrain, T. C. |
author_sort |
Jagdale, G. B. |
title |
Use of Cellulose Acetate Electrophoresis in the Taxonomy of Steinernematids (Rhabditida, Nematoda) |
title_short |
Use of Cellulose Acetate Electrophoresis in the Taxonomy of Steinernematids (Rhabditida, Nematoda) |
title_full |
Use of Cellulose Acetate Electrophoresis in the Taxonomy of Steinernematids (Rhabditida, Nematoda) |
title_fullStr |
Use of Cellulose Acetate Electrophoresis in the Taxonomy of Steinernematids (Rhabditida, Nematoda) |
title_full_unstemmed |
Use of Cellulose Acetate Electrophoresis in the Taxonomy of Steinernematids (Rhabditida, Nematoda) |
title_sort |
use of cellulose acetate electrophoresis in the taxonomy of steinernematids (rhabditida, nematoda) |
publisher |
Journal of Nematology |
publishDate |
1996 |
url |
https://journals.flvc.org/jon/article/view/66821 |
geographic |
Canada |
geographic_facet |
Canada |
genre |
Newfoundland |
genre_facet |
Newfoundland |
op_source |
Journal of Nematology; Vol. 28, No. 3 (September 1996); 301-309 0022-300X |
op_relation |
https://journals.flvc.org/jon/article/view/66821/64489 https://journals.flvc.org/jon/article/view/66821 |
_version_ |
1766107671618912256 |