Nuclear and Mitochondrial DNA Sequence Diversity in the Antarctic Nematode Scottnema lindsayae
A nematode, Scottnema lindsayae, is the dominant metazoan found in soils of the McMurdo Dry Valleys, Antarctica. The distribution of S. lindsayae is patchy within and between these dry valleys; nevertheless, it is unclear to what extent these populations are genetically isolated. We investigated gen...
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Journal of Nematology
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ftfloridaclaojs:oai:ojs.journals.fcla.edu:article/67138 2023-05-15T13:38:57+02:00 Nuclear and Mitochondrial DNA Sequence Diversity in the Antarctic Nematode Scottnema lindsayae Courtright, E. M. Wall, D. H. Virginia, R. A. Frisse, L. M. Vida, J. T. Thomas, W. K. 2000-06-01 application/pdf http://journals.fcla.edu/jon/article/view/67138 eng eng Journal of Nematology http://journals.fcla.edu/jon/article/view/67138/64806 Journal of Nematology; Vol. 32, No. 2 (June 2000); 143-153 0022-300X antarctic; ecology; extreme environment; genetic diversity; genetics; mitochondrial dna; morphology; nematode; ribosomal rna; scottnema lindsayae; soil biodiversity; systematics; taxonomy info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article 2000 ftfloridaclaojs 2017-01-28T00:42:03Z A nematode, Scottnema lindsayae, is the dominant metazoan found in soils of the McMurdo Dry Valleys, Antarctica. The distribution of S. lindsayae is patchy within and between these dry valleys; nevertheless, it is unclear to what extent these populations are genetically isolated. We investigated genetic diversity in this nematode using nuclear and mitochondrial gene sequences that encode ribosomal RNA. In 169 nematodes surveyed, only one variable site was found in each of two different expansion segments of nuclear rRNA. While most nematodes have only one sequence type, some nematodes were found to contain a mixture of both sequences. No fixed differences in nuclear sequences were observed between populations. This pattern of nuclear variation is most consistent with a single species of nematode defined morphologically as S. lindsayae. For mitochondrial DNA sequences, we found 10 variable positions defining 12 haplotypes among 188 nematodes surveyed. While all observed haplotypes are closely related, significant differences in haplotype frequencies were observed between geographically defined populations. The nuclear and mitochondrial variation suggests populations of S. lindsayae represent a single polymorphic species with some restriction of gene flow between geographic populations. Article in Journal/Newspaper Antarc* Antarctic Antarctica McMurdo Dry Valleys Florida Online Journals (FloridaOJ) Antarctic McMurdo Dry Valleys The Antarctic |
institution |
Open Polar |
collection |
Florida Online Journals (FloridaOJ) |
op_collection_id |
ftfloridaclaojs |
language |
English |
topic |
antarctic; ecology; extreme environment; genetic diversity; genetics; mitochondrial dna; morphology; nematode; ribosomal rna; scottnema lindsayae; soil biodiversity; systematics; taxonomy |
spellingShingle |
antarctic; ecology; extreme environment; genetic diversity; genetics; mitochondrial dna; morphology; nematode; ribosomal rna; scottnema lindsayae; soil biodiversity; systematics; taxonomy Courtright, E. M. Wall, D. H. Virginia, R. A. Frisse, L. M. Vida, J. T. Thomas, W. K. Nuclear and Mitochondrial DNA Sequence Diversity in the Antarctic Nematode Scottnema lindsayae |
topic_facet |
antarctic; ecology; extreme environment; genetic diversity; genetics; mitochondrial dna; morphology; nematode; ribosomal rna; scottnema lindsayae; soil biodiversity; systematics; taxonomy |
description |
A nematode, Scottnema lindsayae, is the dominant metazoan found in soils of the McMurdo Dry Valleys, Antarctica. The distribution of S. lindsayae is patchy within and between these dry valleys; nevertheless, it is unclear to what extent these populations are genetically isolated. We investigated genetic diversity in this nematode using nuclear and mitochondrial gene sequences that encode ribosomal RNA. In 169 nematodes surveyed, only one variable site was found in each of two different expansion segments of nuclear rRNA. While most nematodes have only one sequence type, some nematodes were found to contain a mixture of both sequences. No fixed differences in nuclear sequences were observed between populations. This pattern of nuclear variation is most consistent with a single species of nematode defined morphologically as S. lindsayae. For mitochondrial DNA sequences, we found 10 variable positions defining 12 haplotypes among 188 nematodes surveyed. While all observed haplotypes are closely related, significant differences in haplotype frequencies were observed between geographically defined populations. The nuclear and mitochondrial variation suggests populations of S. lindsayae represent a single polymorphic species with some restriction of gene flow between geographic populations. |
format |
Article in Journal/Newspaper |
author |
Courtright, E. M. Wall, D. H. Virginia, R. A. Frisse, L. M. Vida, J. T. Thomas, W. K. |
author_facet |
Courtright, E. M. Wall, D. H. Virginia, R. A. Frisse, L. M. Vida, J. T. Thomas, W. K. |
author_sort |
Courtright, E. M. |
title |
Nuclear and Mitochondrial DNA Sequence Diversity in the Antarctic Nematode Scottnema lindsayae |
title_short |
Nuclear and Mitochondrial DNA Sequence Diversity in the Antarctic Nematode Scottnema lindsayae |
title_full |
Nuclear and Mitochondrial DNA Sequence Diversity in the Antarctic Nematode Scottnema lindsayae |
title_fullStr |
Nuclear and Mitochondrial DNA Sequence Diversity in the Antarctic Nematode Scottnema lindsayae |
title_full_unstemmed |
Nuclear and Mitochondrial DNA Sequence Diversity in the Antarctic Nematode Scottnema lindsayae |
title_sort |
nuclear and mitochondrial dna sequence diversity in the antarctic nematode scottnema lindsayae |
publisher |
Journal of Nematology |
publishDate |
2000 |
url |
http://journals.fcla.edu/jon/article/view/67138 |
geographic |
Antarctic McMurdo Dry Valleys The Antarctic |
geographic_facet |
Antarctic McMurdo Dry Valleys The Antarctic |
genre |
Antarc* Antarctic Antarctica McMurdo Dry Valleys |
genre_facet |
Antarc* Antarctic Antarctica McMurdo Dry Valleys |
op_source |
Journal of Nematology; Vol. 32, No. 2 (June 2000); 143-153 0022-300X |
op_relation |
http://journals.fcla.edu/jon/article/view/67138/64806 |
_version_ |
1766112891196407808 |