Carbohydrates and carbohydrate degradation gene abundance and transcription in Atlantic waters of the Arctic
Carbohydrates are chemically and structurally diverse, represent a substantial fraction of marine organic matter and are key substrates for heterotrophic microbes. Studies on carbohydrate utilisation by marine microbes have been centred on phytoplankton blooms in temperate regions, while far less is...
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ftethz:oai:www.research-collection.ethz.ch:20.500.11850/649070 2024-01-21T10:03:25+01:00 Carbohydrates and carbohydrate degradation gene abundance and transcription in Atlantic waters of the Arctic Priest, Taylor id_orcid:0 000-0003-4173-8354 Vidal-Melgosa, Silvia Hehemann, Jan-Hendrik Amann, Rudolf Fuchs, Bernhard M. 2023-12-09 application/application/pdf https://hdl.handle.net/20.500.11850/649070 https://doi.org/10.3929/ethz-b-000649070 en eng Nature info:eu-repo/semantics/altIdentifier/doi/10.1038/s43705-023-00324-7 info:eu-repo/semantics/altIdentifier/wos/001117655700001 http://hdl.handle.net/20.500.11850/649070 doi:10.3929/ethz-b-000649070 info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International ISME Communications, 3 (1) info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2023 ftethz https://doi.org/20.500.11850/64907010.3929/ethz-b-00064907010.1038/s43705-023-00324-7 2023-12-25T00:52:07Z Carbohydrates are chemically and structurally diverse, represent a substantial fraction of marine organic matter and are key substrates for heterotrophic microbes. Studies on carbohydrate utilisation by marine microbes have been centred on phytoplankton blooms in temperate regions, while far less is known from high-latitude waters and during later seasonal stages. Here, we combine glycan microarrays and analytical chromatography with metagenomics and metatranscriptomics to show the spatial heterogeneity in glycan distribution and potential carbohydrate utilisation by microbes in Atlantic waters of the Arctic. The composition and abundance of monomers and glycan structures in POM varied with location and depth. Complex fucose-containing sulfated polysaccharides, known to accumulate in the ocean, were consistently detected, while the more labile beta-1,3-glucan exhibited a patchy distribution. Through 'omics analysis, we identify variations in the abundance and transcription of carbohydrate degradation-related genes across samples at the community and population level. The populations contributing the most to transcription were taxonomically related to those known as primary responders and key carbohydrate degraders in temperate ecosystems, such as NS4 Marine Group and Formosa. The unique transcription profiles for these populations suggest distinct substrate utilisation potentials, with predicted glycan targets corresponding to those structurally identified in POM from the same sampling sites. By combining cutting-edge technologies and protocols, we provide insights into the carbohydrate component of the carbon cycle in the Arctic during late summer and present a high-quality dataset that will be of great value for future comparative analyses. ISSN:2730-6151 Article in Journal/Newspaper Arctic Phytoplankton ETH Zürich Research Collection Arctic |
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Open Polar |
collection |
ETH Zürich Research Collection |
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ftethz |
language |
English |
description |
Carbohydrates are chemically and structurally diverse, represent a substantial fraction of marine organic matter and are key substrates for heterotrophic microbes. Studies on carbohydrate utilisation by marine microbes have been centred on phytoplankton blooms in temperate regions, while far less is known from high-latitude waters and during later seasonal stages. Here, we combine glycan microarrays and analytical chromatography with metagenomics and metatranscriptomics to show the spatial heterogeneity in glycan distribution and potential carbohydrate utilisation by microbes in Atlantic waters of the Arctic. The composition and abundance of monomers and glycan structures in POM varied with location and depth. Complex fucose-containing sulfated polysaccharides, known to accumulate in the ocean, were consistently detected, while the more labile beta-1,3-glucan exhibited a patchy distribution. Through 'omics analysis, we identify variations in the abundance and transcription of carbohydrate degradation-related genes across samples at the community and population level. The populations contributing the most to transcription were taxonomically related to those known as primary responders and key carbohydrate degraders in temperate ecosystems, such as NS4 Marine Group and Formosa. The unique transcription profiles for these populations suggest distinct substrate utilisation potentials, with predicted glycan targets corresponding to those structurally identified in POM from the same sampling sites. By combining cutting-edge technologies and protocols, we provide insights into the carbohydrate component of the carbon cycle in the Arctic during late summer and present a high-quality dataset that will be of great value for future comparative analyses. ISSN:2730-6151 |
format |
Article in Journal/Newspaper |
author |
Priest, Taylor id_orcid:0 000-0003-4173-8354 Vidal-Melgosa, Silvia Hehemann, Jan-Hendrik Amann, Rudolf Fuchs, Bernhard M. |
spellingShingle |
Priest, Taylor id_orcid:0 000-0003-4173-8354 Vidal-Melgosa, Silvia Hehemann, Jan-Hendrik Amann, Rudolf Fuchs, Bernhard M. Carbohydrates and carbohydrate degradation gene abundance and transcription in Atlantic waters of the Arctic |
author_facet |
Priest, Taylor id_orcid:0 000-0003-4173-8354 Vidal-Melgosa, Silvia Hehemann, Jan-Hendrik Amann, Rudolf Fuchs, Bernhard M. |
author_sort |
Priest, Taylor |
title |
Carbohydrates and carbohydrate degradation gene abundance and transcription in Atlantic waters of the Arctic |
title_short |
Carbohydrates and carbohydrate degradation gene abundance and transcription in Atlantic waters of the Arctic |
title_full |
Carbohydrates and carbohydrate degradation gene abundance and transcription in Atlantic waters of the Arctic |
title_fullStr |
Carbohydrates and carbohydrate degradation gene abundance and transcription in Atlantic waters of the Arctic |
title_full_unstemmed |
Carbohydrates and carbohydrate degradation gene abundance and transcription in Atlantic waters of the Arctic |
title_sort |
carbohydrates and carbohydrate degradation gene abundance and transcription in atlantic waters of the arctic |
publisher |
Nature |
publishDate |
2023 |
url |
https://hdl.handle.net/20.500.11850/649070 https://doi.org/10.3929/ethz-b-000649070 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Phytoplankton |
genre_facet |
Arctic Phytoplankton |
op_source |
ISME Communications, 3 (1) |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1038/s43705-023-00324-7 info:eu-repo/semantics/altIdentifier/wos/001117655700001 http://hdl.handle.net/20.500.11850/649070 doi:10.3929/ethz-b-000649070 |
op_rights |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International |
op_doi |
https://doi.org/20.500.11850/64907010.3929/ethz-b-00064907010.1038/s43705-023-00324-7 |
_version_ |
1788693701652905984 |