A novel nematode species from the Siberian permafrost shares adaptive mechanisms for cryptobiotic survival with C. elegans dauer larva
Some organisms in nature have developed the ability to enter a state of suspended metabolism called cryptobiosis when environmental conditions are unfavorable. This state-transition requires execution of a combination of genetic and biochemical pathways that enable the organism to survive for prolon...
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ftethz:oai:www.research-collection.ethz.ch:20.500.11850/625578 2023-10-01T03:58:52+02:00 A novel nematode species from the Siberian permafrost shares adaptive mechanisms for cryptobiotic survival with C. elegans dauer larva Shatilovich, Anastasia Gade, Vamshidhar R. Pippel, Martin Hoffmeyer, Tarja T. Tchesunov, Alexei V. Stevens, Lewis Winkler, Sylke Hughes, Graham M. Traikov, Sofia Hiller, Michael Rivkina, Elizaveta Schiffer, Philipp H. Myers, Eugene W. Kurzchalia, Teymuras V. 2023-07 application/application/pdf https://hdl.handle.net/20.500.11850/625578 https://doi.org/10.3929/ethz-b-000625578 en eng PLOS info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pgen.1010798 info:eu-repo/semantics/altIdentifier/wos/001041133100001 http://hdl.handle.net/20.500.11850/625578 doi:10.3929/ethz-b-000625578 info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International PLoS Genetics, 19 (7) info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2023 ftethz https://doi.org/20.500.11850/62557810.3929/ethz-b-00062557810.1371/journal.pgen.1010798 2023-09-03T23:49:36Z Some organisms in nature have developed the ability to enter a state of suspended metabolism called cryptobiosis when environmental conditions are unfavorable. This state-transition requires execution of a combination of genetic and biochemical pathways that enable the organism to survive for prolonged periods. Recently, nematode individuals have been reanimated from Siberian permafrost after remaining in cryptobiosis. Preliminary analysis indicates that these nematodes belong to the genera Panagrolaimus and Plectus. Here, we present precise radiocarbon dating indicating that the Panagrolaimus individuals have remained in cryptobiosis since the late Pleistocene (~46,000 years). Phylogenetic inference based on our genome assembly and a detailed morphological analysis demonstrate that they belong to an undescribed species, which we named Panagrolaimus kolymaensis. Comparative genome analysis revealed that the molecular toolkit for cryptobiosis in P. kolymaensis and in C. elegans is partly orthologous. We show that biochemical mechanisms employed by these two species to survive desiccation and freezing under laboratory conditions are similar. Our experimental evidence also reveals that C. elegans dauer larvae can remain viable for longer periods in suspended animation than previously reported. Altogether, our findings demonstrate that nematodes evolved mechanisms potentially allowing them to suspend life over geological time scales. ISSN:1553-7390 ISSN:1553-7404 Article in Journal/Newspaper permafrost ETH Zürich Research Collection |
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Open Polar |
collection |
ETH Zürich Research Collection |
op_collection_id |
ftethz |
language |
English |
description |
Some organisms in nature have developed the ability to enter a state of suspended metabolism called cryptobiosis when environmental conditions are unfavorable. This state-transition requires execution of a combination of genetic and biochemical pathways that enable the organism to survive for prolonged periods. Recently, nematode individuals have been reanimated from Siberian permafrost after remaining in cryptobiosis. Preliminary analysis indicates that these nematodes belong to the genera Panagrolaimus and Plectus. Here, we present precise radiocarbon dating indicating that the Panagrolaimus individuals have remained in cryptobiosis since the late Pleistocene (~46,000 years). Phylogenetic inference based on our genome assembly and a detailed morphological analysis demonstrate that they belong to an undescribed species, which we named Panagrolaimus kolymaensis. Comparative genome analysis revealed that the molecular toolkit for cryptobiosis in P. kolymaensis and in C. elegans is partly orthologous. We show that biochemical mechanisms employed by these two species to survive desiccation and freezing under laboratory conditions are similar. Our experimental evidence also reveals that C. elegans dauer larvae can remain viable for longer periods in suspended animation than previously reported. Altogether, our findings demonstrate that nematodes evolved mechanisms potentially allowing them to suspend life over geological time scales. ISSN:1553-7390 ISSN:1553-7404 |
format |
Article in Journal/Newspaper |
author |
Shatilovich, Anastasia Gade, Vamshidhar R. Pippel, Martin Hoffmeyer, Tarja T. Tchesunov, Alexei V. Stevens, Lewis Winkler, Sylke Hughes, Graham M. Traikov, Sofia Hiller, Michael Rivkina, Elizaveta Schiffer, Philipp H. Myers, Eugene W. Kurzchalia, Teymuras V. |
spellingShingle |
Shatilovich, Anastasia Gade, Vamshidhar R. Pippel, Martin Hoffmeyer, Tarja T. Tchesunov, Alexei V. Stevens, Lewis Winkler, Sylke Hughes, Graham M. Traikov, Sofia Hiller, Michael Rivkina, Elizaveta Schiffer, Philipp H. Myers, Eugene W. Kurzchalia, Teymuras V. A novel nematode species from the Siberian permafrost shares adaptive mechanisms for cryptobiotic survival with C. elegans dauer larva |
author_facet |
Shatilovich, Anastasia Gade, Vamshidhar R. Pippel, Martin Hoffmeyer, Tarja T. Tchesunov, Alexei V. Stevens, Lewis Winkler, Sylke Hughes, Graham M. Traikov, Sofia Hiller, Michael Rivkina, Elizaveta Schiffer, Philipp H. Myers, Eugene W. Kurzchalia, Teymuras V. |
author_sort |
Shatilovich, Anastasia |
title |
A novel nematode species from the Siberian permafrost shares adaptive mechanisms for cryptobiotic survival with C. elegans dauer larva |
title_short |
A novel nematode species from the Siberian permafrost shares adaptive mechanisms for cryptobiotic survival with C. elegans dauer larva |
title_full |
A novel nematode species from the Siberian permafrost shares adaptive mechanisms for cryptobiotic survival with C. elegans dauer larva |
title_fullStr |
A novel nematode species from the Siberian permafrost shares adaptive mechanisms for cryptobiotic survival with C. elegans dauer larva |
title_full_unstemmed |
A novel nematode species from the Siberian permafrost shares adaptive mechanisms for cryptobiotic survival with C. elegans dauer larva |
title_sort |
novel nematode species from the siberian permafrost shares adaptive mechanisms for cryptobiotic survival with c. elegans dauer larva |
publisher |
PLOS |
publishDate |
2023 |
url |
https://hdl.handle.net/20.500.11850/625578 https://doi.org/10.3929/ethz-b-000625578 |
genre |
permafrost |
genre_facet |
permafrost |
op_source |
PLoS Genetics, 19 (7) |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pgen.1010798 info:eu-repo/semantics/altIdentifier/wos/001041133100001 http://hdl.handle.net/20.500.11850/625578 doi:10.3929/ethz-b-000625578 |
op_rights |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International |
op_doi |
https://doi.org/20.500.11850/62557810.3929/ethz-b-00062557810.1371/journal.pgen.1010798 |
_version_ |
1778532093269114880 |