Shotgun metagenomics reveals distinct functional diversity and metabolic capabilities between 12 000-year-old permafrost and active layers on Muot da Barba Peider (Swiss Alps)
The warming-induced thawing of permafrost promotes microbial activity, often resulting in enhanced greenhouse gas emissions. The ability of permafrost microorganisms to survive the in situ sub-zero temperatures, their energetic strategies and their metabolic versatility in using soil organic materia...
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ftethz:oai:www.research-collection.ethz.ch:20.500.11850/480369 2023-05-15T17:55:21+02:00 Shotgun metagenomics reveals distinct functional diversity and metabolic capabilities between 12 000-year-old permafrost and active layers on Muot da Barba Peider (Swiss Alps) Perez-Mon, Carla Qi, Weihong Vikram, Surendra Frossard, Aline Makhalanyane, Thulani Cowan, Don Frey, Beat 2021 application/application/pdf https://hdl.handle.net/20.500.11850/480369 https://doi.org/10.3929/ethz-b-000480369 en eng Microbiology Society info:eu-repo/semantics/altIdentifier/doi/10.1099/mgen.0.000558 http://hdl.handle.net/20.500.11850/480369 doi:10.3929/ethz-b-000480369 info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International CC-BY Microbial Genomics, 7 (4) alpine metagenomics microbial communities permafrost soil and warming info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2021 ftethz https://doi.org/20.500.11850/480369 https://doi.org/10.3929/ethz-b-000480369 https://doi.org/10.1099/mgen.0.000558 2023-02-13T00:55:12Z The warming-induced thawing of permafrost promotes microbial activity, often resulting in enhanced greenhouse gas emissions. The ability of permafrost microorganisms to survive the in situ sub-zero temperatures, their energetic strategies and their metabolic versatility in using soil organic materials determine their growth and functionality upon thawing. Hence, functional characterization of the permafrost microbiome, particularly in the underexplored mid-latitudinal alpine regions, is a crucial first step in predicting its responses to the changing climate, and the consequences for soil–climate feedbacks. In this study, for the first time, the functional potential and metabolic capabilities of a temperate mountain permafrost microbiome from central Europe has been analysed using shotgun metagenomics. Permafrost and active layers from the summit of Muot da Barba Peider (MBP) [Swiss Alps, 2979 m above sea level (a.s.l.)] revealed a strikingly high functional diversity in the permafrost (north-facing soils at a depth of 160 cm). Permafrost metagenomes were enriched in stress-response genes (e.g. cold-shock genes, chaperones), as well as in genes involved in cell defence and competition (e.g. antiviral proteins, antibiotics, motility, nutrient-uptake ABC transporters), compared with active-layer metagenomes. Permafrost also showed a higher potential for the synthesis of carbohydrate-active enzymes, and an overrepresentation of genes involved in fermentation, carbon fixation, denitrification and nitrogen reduction reactions. Collectively, these findings demonstrate the potential capabilities of permafrost microorganisms to thrive in cold and oligotrophic conditions, and highlight their metabolic versatility in carbon and nitrogen cycling. Our study provides a first insight into the high functional gene diversity of the central European mountain permafrost microbiome. Our findings extend our understanding of the microbial ecology of permafrost and represent a baseline for future investigations comparing the ... Article in Journal/Newspaper permafrost ETH Zürich Research Collection |
institution |
Open Polar |
collection |
ETH Zürich Research Collection |
op_collection_id |
ftethz |
language |
English |
topic |
alpine metagenomics microbial communities permafrost soil and warming |
spellingShingle |
alpine metagenomics microbial communities permafrost soil and warming Perez-Mon, Carla Qi, Weihong Vikram, Surendra Frossard, Aline Makhalanyane, Thulani Cowan, Don Frey, Beat Shotgun metagenomics reveals distinct functional diversity and metabolic capabilities between 12 000-year-old permafrost and active layers on Muot da Barba Peider (Swiss Alps) |
topic_facet |
alpine metagenomics microbial communities permafrost soil and warming |
description |
The warming-induced thawing of permafrost promotes microbial activity, often resulting in enhanced greenhouse gas emissions. The ability of permafrost microorganisms to survive the in situ sub-zero temperatures, their energetic strategies and their metabolic versatility in using soil organic materials determine their growth and functionality upon thawing. Hence, functional characterization of the permafrost microbiome, particularly in the underexplored mid-latitudinal alpine regions, is a crucial first step in predicting its responses to the changing climate, and the consequences for soil–climate feedbacks. In this study, for the first time, the functional potential and metabolic capabilities of a temperate mountain permafrost microbiome from central Europe has been analysed using shotgun metagenomics. Permafrost and active layers from the summit of Muot da Barba Peider (MBP) [Swiss Alps, 2979 m above sea level (a.s.l.)] revealed a strikingly high functional diversity in the permafrost (north-facing soils at a depth of 160 cm). Permafrost metagenomes were enriched in stress-response genes (e.g. cold-shock genes, chaperones), as well as in genes involved in cell defence and competition (e.g. antiviral proteins, antibiotics, motility, nutrient-uptake ABC transporters), compared with active-layer metagenomes. Permafrost also showed a higher potential for the synthesis of carbohydrate-active enzymes, and an overrepresentation of genes involved in fermentation, carbon fixation, denitrification and nitrogen reduction reactions. Collectively, these findings demonstrate the potential capabilities of permafrost microorganisms to thrive in cold and oligotrophic conditions, and highlight their metabolic versatility in carbon and nitrogen cycling. Our study provides a first insight into the high functional gene diversity of the central European mountain permafrost microbiome. Our findings extend our understanding of the microbial ecology of permafrost and represent a baseline for future investigations comparing the ... |
format |
Article in Journal/Newspaper |
author |
Perez-Mon, Carla Qi, Weihong Vikram, Surendra Frossard, Aline Makhalanyane, Thulani Cowan, Don Frey, Beat |
author_facet |
Perez-Mon, Carla Qi, Weihong Vikram, Surendra Frossard, Aline Makhalanyane, Thulani Cowan, Don Frey, Beat |
author_sort |
Perez-Mon, Carla |
title |
Shotgun metagenomics reveals distinct functional diversity and metabolic capabilities between 12 000-year-old permafrost and active layers on Muot da Barba Peider (Swiss Alps) |
title_short |
Shotgun metagenomics reveals distinct functional diversity and metabolic capabilities between 12 000-year-old permafrost and active layers on Muot da Barba Peider (Swiss Alps) |
title_full |
Shotgun metagenomics reveals distinct functional diversity and metabolic capabilities between 12 000-year-old permafrost and active layers on Muot da Barba Peider (Swiss Alps) |
title_fullStr |
Shotgun metagenomics reveals distinct functional diversity and metabolic capabilities between 12 000-year-old permafrost and active layers on Muot da Barba Peider (Swiss Alps) |
title_full_unstemmed |
Shotgun metagenomics reveals distinct functional diversity and metabolic capabilities between 12 000-year-old permafrost and active layers on Muot da Barba Peider (Swiss Alps) |
title_sort |
shotgun metagenomics reveals distinct functional diversity and metabolic capabilities between 12 000-year-old permafrost and active layers on muot da barba peider (swiss alps) |
publisher |
Microbiology Society |
publishDate |
2021 |
url |
https://hdl.handle.net/20.500.11850/480369 https://doi.org/10.3929/ethz-b-000480369 |
genre |
permafrost |
genre_facet |
permafrost |
op_source |
Microbial Genomics, 7 (4) |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1099/mgen.0.000558 http://hdl.handle.net/20.500.11850/480369 doi:10.3929/ethz-b-000480369 |
op_rights |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/20.500.11850/480369 https://doi.org/10.3929/ethz-b-000480369 https://doi.org/10.1099/mgen.0.000558 |
_version_ |
1766163281796399104 |