Uterine responses to early pre-attachment embryos in the domestic dog and comparisons with other domestic animal species
In the dog, there is no luteolysis in the absence of pregnancy. Thus, this species lacks any anti-luteolytic endocrine signal as found in other species that modulate uterine function during the critical period of pregnancy establishment. Nevertheless, in the dog an embryo-maternal communication must...
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ftethz:oai:www.research-collection.ethz.ch:20.500.11850/220083 2023-05-15T15:50:58+02:00 Uterine responses to early pre-attachment embryos in the domestic dog and comparisons with other domestic animal species Graubner, Felix R. Gram, Aykut Kautz, Ewa Bauersachs, Stefan Aslan, Selim Agaoglu, Ali R. Boos, Alois Kowalewski, Mariusz P. 2017-08 application/application/pdf https://hdl.handle.net/20.500.11850/220083 https://doi.org/10.3929/ethz-b-000220083 en eng Oxford University Press info:eu-repo/semantics/altIdentifier/doi/10.1093/biolre/iox063 info:eu-repo/semantics/altIdentifier/wos/000413005500005 http://hdl.handle.net/20.500.11850/220083 doi:10.3929/ethz-b-000220083 info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International CC-BY Biology of Reproduction, 97 (2) dog (Canis lupus familiaris) embryo-maternal communication early pregnancy info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2017 ftethz https://doi.org/20.500.11850/220083 https://doi.org/10.3929/ethz-b-000220083 https://doi.org/10.1093/biolre/iox063 2022-04-25T13:20:15Z In the dog, there is no luteolysis in the absence of pregnancy. Thus, this species lacks any anti-luteolytic endocrine signal as found in other species that modulate uterine function during the critical period of pregnancy establishment. Nevertheless, in the dog an embryo-maternal communication must occur in order to prevent rejection of embryos. Based on this hypothesis, we performed microarray analysis of canine uterine samples collected during pre-attachment phase (days 10-12) and in corresponding non-pregnant controls, in order to elucidate the embryo attachment signal. An additional goal was to identify differences in uterine responses to pre-attachment embryos between dogs and other mammalian species exhibiting different reproductive patterns with regard to luteolysis, implantation, and preparation for placentation. Therefore, the canine microarray data were compared with gene sets from pigs, cattle, horses, and humans. We found 412 genes differentially regulated between the two experimental groups. The functional terms most strongly enriched in response to pre-attachment embryos related to extracellular matrix function and remodeling, and to immune and inflammatory responses. Several candidate genes were validated by semi-quantitative PCR. When compared with other species, best matches were found with human and equine counterparts. Especially for the pig, the majority of overlapping genes showed opposite expression patterns. Interestingly, 1926 genes did not pair with any of the other gene sets. Using a microarray approach, we report the uterine changes in the dog driven by the presence of embryos and compare these results with datasets from other mammalian species, finding common-, contrary-, and exclusively canine-regulated genes. ISSN:0006-3363 ISSN:1529-7268 Article in Journal/Newspaper Canis lupus ETH Zürich Research Collection |
institution |
Open Polar |
collection |
ETH Zürich Research Collection |
op_collection_id |
ftethz |
language |
English |
topic |
dog (Canis lupus familiaris) embryo-maternal communication early pregnancy |
spellingShingle |
dog (Canis lupus familiaris) embryo-maternal communication early pregnancy Graubner, Felix R. Gram, Aykut Kautz, Ewa Bauersachs, Stefan Aslan, Selim Agaoglu, Ali R. Boos, Alois Kowalewski, Mariusz P. Uterine responses to early pre-attachment embryos in the domestic dog and comparisons with other domestic animal species |
topic_facet |
dog (Canis lupus familiaris) embryo-maternal communication early pregnancy |
description |
In the dog, there is no luteolysis in the absence of pregnancy. Thus, this species lacks any anti-luteolytic endocrine signal as found in other species that modulate uterine function during the critical period of pregnancy establishment. Nevertheless, in the dog an embryo-maternal communication must occur in order to prevent rejection of embryos. Based on this hypothesis, we performed microarray analysis of canine uterine samples collected during pre-attachment phase (days 10-12) and in corresponding non-pregnant controls, in order to elucidate the embryo attachment signal. An additional goal was to identify differences in uterine responses to pre-attachment embryos between dogs and other mammalian species exhibiting different reproductive patterns with regard to luteolysis, implantation, and preparation for placentation. Therefore, the canine microarray data were compared with gene sets from pigs, cattle, horses, and humans. We found 412 genes differentially regulated between the two experimental groups. The functional terms most strongly enriched in response to pre-attachment embryos related to extracellular matrix function and remodeling, and to immune and inflammatory responses. Several candidate genes were validated by semi-quantitative PCR. When compared with other species, best matches were found with human and equine counterparts. Especially for the pig, the majority of overlapping genes showed opposite expression patterns. Interestingly, 1926 genes did not pair with any of the other gene sets. Using a microarray approach, we report the uterine changes in the dog driven by the presence of embryos and compare these results with datasets from other mammalian species, finding common-, contrary-, and exclusively canine-regulated genes. ISSN:0006-3363 ISSN:1529-7268 |
format |
Article in Journal/Newspaper |
author |
Graubner, Felix R. Gram, Aykut Kautz, Ewa Bauersachs, Stefan Aslan, Selim Agaoglu, Ali R. Boos, Alois Kowalewski, Mariusz P. |
author_facet |
Graubner, Felix R. Gram, Aykut Kautz, Ewa Bauersachs, Stefan Aslan, Selim Agaoglu, Ali R. Boos, Alois Kowalewski, Mariusz P. |
author_sort |
Graubner, Felix R. |
title |
Uterine responses to early pre-attachment embryos in the domestic dog and comparisons with other domestic animal species |
title_short |
Uterine responses to early pre-attachment embryos in the domestic dog and comparisons with other domestic animal species |
title_full |
Uterine responses to early pre-attachment embryos in the domestic dog and comparisons with other domestic animal species |
title_fullStr |
Uterine responses to early pre-attachment embryos in the domestic dog and comparisons with other domestic animal species |
title_full_unstemmed |
Uterine responses to early pre-attachment embryos in the domestic dog and comparisons with other domestic animal species |
title_sort |
uterine responses to early pre-attachment embryos in the domestic dog and comparisons with other domestic animal species |
publisher |
Oxford University Press |
publishDate |
2017 |
url |
https://hdl.handle.net/20.500.11850/220083 https://doi.org/10.3929/ethz-b-000220083 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
Biology of Reproduction, 97 (2) |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1093/biolre/iox063 info:eu-repo/semantics/altIdentifier/wos/000413005500005 http://hdl.handle.net/20.500.11850/220083 doi:10.3929/ethz-b-000220083 |
op_rights |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/20.500.11850/220083 https://doi.org/10.3929/ethz-b-000220083 https://doi.org/10.1093/biolre/iox063 |
_version_ |
1766385999960604672 |