Microbial diversity in European alpine permafrost and active layers
Permafrost represents a largely understudied genetic resource. Thawing of permafrost with global warming will not only promote microbial carbon turnover with direct feedback on greenhouse gases, but also unlock an unknown microbial diversity. Pioneering metagenomic efforts have shed light on the per...
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ftethz:oai:www.research-collection.ethz.ch:20.500.11850/114910 2023-06-18T03:42:37+02:00 Microbial diversity in European alpine permafrost and active layers Frey, Beat Rime, Thomas Phillips, Marcia Stierli, Beat Hajdas, Irka Widmer, Franco Hartmann, Martin 2016-03 application/application/pdf https://hdl.handle.net/20.500.11850/114910 https://doi.org/10.3929/ethz-b-000114910 en eng Oxford University Press info:eu-repo/semantics/altIdentifier/doi/10.1093/femsec/fiw018 info:eu-repo/semantics/altIdentifier/wos/000373295800012 http://hdl.handle.net/20.500.11850/114910 doi:10.3929/ethz-b-000114910 info:eu-repo/semantics/openAccess http://rightsstatements.org/page/InC-NC/1.0/ In Copyright - Non-Commercial Use Permitted FEMS Microbiology Ecology, 92 (3) Alpine permafrost Archaea Bacteria Eukarya Illumina Miseq sequencing Novel microbial diversity Ribosomal markers info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2016 ftethz https://doi.org/20.500.11850/11491010.3929/ethz-b-00011491010.1093/femsec/fiw018 2023-06-04T23:49:28Z Permafrost represents a largely understudied genetic resource. Thawing of permafrost with global warming will not only promote microbial carbon turnover with direct feedback on greenhouse gases, but also unlock an unknown microbial diversity. Pioneering metagenomic efforts have shed light on the permafrost microbiome in polar regions, but temperate mountain permafrost is largely understudied. We applied a unique experimental design coupled to high-throughput sequencing of ribosomal markers to characterize the microbiota at the long-term alpine permafrost study site ‘Muot-da-Barba-Peider’ in eastern Switzerland with an approximate radiocarbon age of 12 000 years. Compared to the active layers, the permafrost community was more diverse and enriched with members of the superphylum Patescibacteria (OD1, TM7, GN02 and OP11). These understudied phyla with no cultured representatives proposedly feature small streamlined genomes with reduced metabolic capabilities, adaptations to anaerobic fermentative metabolisms and potential ectosymbiotic lifestyles. The permafrost microbiota was also enriched with yeasts and lichenized fungi known to harbour various structural and functional adaptation mechanisms to survive under extreme sub-zero conditions. These data yield an unprecedented view on microbial life in temperate mountain permafrost, which is increasingly important for understanding the biological dynamics of permafrost in order to anticipate potential ecological trajectories in a warming world. ISSN:0168-6496 ISSN:1574-6941 Article in Journal/Newspaper permafrost ETH Zürich Research Collection Geology 49 9 1117 1121 |
institution |
Open Polar |
collection |
ETH Zürich Research Collection |
op_collection_id |
ftethz |
language |
English |
topic |
Alpine permafrost Archaea Bacteria Eukarya Illumina Miseq sequencing Novel microbial diversity Ribosomal markers |
spellingShingle |
Alpine permafrost Archaea Bacteria Eukarya Illumina Miseq sequencing Novel microbial diversity Ribosomal markers Frey, Beat Rime, Thomas Phillips, Marcia Stierli, Beat Hajdas, Irka Widmer, Franco Hartmann, Martin Microbial diversity in European alpine permafrost and active layers |
topic_facet |
Alpine permafrost Archaea Bacteria Eukarya Illumina Miseq sequencing Novel microbial diversity Ribosomal markers |
description |
Permafrost represents a largely understudied genetic resource. Thawing of permafrost with global warming will not only promote microbial carbon turnover with direct feedback on greenhouse gases, but also unlock an unknown microbial diversity. Pioneering metagenomic efforts have shed light on the permafrost microbiome in polar regions, but temperate mountain permafrost is largely understudied. We applied a unique experimental design coupled to high-throughput sequencing of ribosomal markers to characterize the microbiota at the long-term alpine permafrost study site ‘Muot-da-Barba-Peider’ in eastern Switzerland with an approximate radiocarbon age of 12 000 years. Compared to the active layers, the permafrost community was more diverse and enriched with members of the superphylum Patescibacteria (OD1, TM7, GN02 and OP11). These understudied phyla with no cultured representatives proposedly feature small streamlined genomes with reduced metabolic capabilities, adaptations to anaerobic fermentative metabolisms and potential ectosymbiotic lifestyles. The permafrost microbiota was also enriched with yeasts and lichenized fungi known to harbour various structural and functional adaptation mechanisms to survive under extreme sub-zero conditions. These data yield an unprecedented view on microbial life in temperate mountain permafrost, which is increasingly important for understanding the biological dynamics of permafrost in order to anticipate potential ecological trajectories in a warming world. ISSN:0168-6496 ISSN:1574-6941 |
format |
Article in Journal/Newspaper |
author |
Frey, Beat Rime, Thomas Phillips, Marcia Stierli, Beat Hajdas, Irka Widmer, Franco Hartmann, Martin |
author_facet |
Frey, Beat Rime, Thomas Phillips, Marcia Stierli, Beat Hajdas, Irka Widmer, Franco Hartmann, Martin |
author_sort |
Frey, Beat |
title |
Microbial diversity in European alpine permafrost and active layers |
title_short |
Microbial diversity in European alpine permafrost and active layers |
title_full |
Microbial diversity in European alpine permafrost and active layers |
title_fullStr |
Microbial diversity in European alpine permafrost and active layers |
title_full_unstemmed |
Microbial diversity in European alpine permafrost and active layers |
title_sort |
microbial diversity in european alpine permafrost and active layers |
publisher |
Oxford University Press |
publishDate |
2016 |
url |
https://hdl.handle.net/20.500.11850/114910 https://doi.org/10.3929/ethz-b-000114910 |
genre |
permafrost |
genre_facet |
permafrost |
op_source |
FEMS Microbiology Ecology, 92 (3) |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1093/femsec/fiw018 info:eu-repo/semantics/altIdentifier/wos/000373295800012 http://hdl.handle.net/20.500.11850/114910 doi:10.3929/ethz-b-000114910 |
op_rights |
info:eu-repo/semantics/openAccess http://rightsstatements.org/page/InC-NC/1.0/ In Copyright - Non-Commercial Use Permitted |
op_doi |
https://doi.org/20.500.11850/11491010.3929/ethz-b-00011491010.1093/femsec/fiw018 |
container_title |
Geology |
container_volume |
49 |
container_issue |
9 |
container_start_page |
1117 |
op_container_end_page |
1121 |
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1769008597567537152 |