Manifestation of Heat-Induced Valuable Dietary Nucleotide Salvage in Food Prepared from Aged Fish in Fast Protein and Metabolites Liquid Chromatography, ATP-Bioluminescence Assay, and NMR Spectra
Dietary nucleotides and nucleosides, primarily inosine monophosphate (IMP) and the adenine nucleotide pool (ANP), are widely considered as essential nutrients responsible for multiple biological functions. Food prepared from meat and fish is the main source of these substances in the human diet, and...
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Online Access: | http://hdl.handle.net/10492/8669 https://doi.org/10.3390/appliedchem3020021 |
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ftestonianunivls:oai:dspace.emu.ee:10492/8669 2023-12-10T09:39:09+01:00 Manifestation of Heat-Induced Valuable Dietary Nucleotide Salvage in Food Prepared from Aged Fish in Fast Protein and Metabolites Liquid Chromatography, ATP-Bioluminescence Assay, and NMR Spectra Sünter, Alar Kuznetsov, Artur Rausepp, Piret Püssa, Tõnu Toom, Lauri Konoplev, Georgii Stepanova, Oksana S. Stepanova, Oksana V. Lyalin, Daniil Frorip, Aleksandr Roasto, Mati 2023 application/pdf http://hdl.handle.net/10492/8669 https://doi.org/10.3390/appliedchem3020021 unknown MDPI This work was supported by the Estonian Research Council grant (PRG1441). AppliedChem 2023, 3, 334–34 2673-9623 http://hdl.handle.net/10492/8669 https://doi.org/10.3390/appliedchem3020021 Sünter, A.; Kuznetsov, A.; Raudsepp, P.; Püssa, T.; Toom, L.; Konoplev, G.; Stepanova, O.S.; Stepanova, O.V.; Lyalin, D.; Frorip, A.; et al. Manifestation of Heat-Induced Valuable Dietary Nucleotide Salvage in Food Prepared from Aged Fish in Fast Protein and Metabolites Liquid Chromatography, ATP-Bioluminescence Assay, and NMR Spectra. AppliedChem 2023, 3, 334–349. https://doi.org/10.3390/ appliedchem3020021 Creative Commons Attribution 4.0 International (CC BY 4.0) © 2023 by the authors. info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ nuclear magnetic resonance fast protein liquid chromatography fish freshness dietary nucleotides nucleotide salvage articles info:eu-repo/semantics/article 2023 ftestonianunivls https://doi.org/10.3390/appliedchem3020021 2023-11-16T00:18:17Z Dietary nucleotides and nucleosides, primarily inosine monophosphate (IMP) and the adenine nucleotide pool (ANP), are widely considered as essential nutrients responsible for multiple biological functions. Food prepared from meat and fish is the main source of these substances in the human diet, and it is extremely important to implement storage and processing techniques ensuring their maximum preservation and even accumulation during maturation or conditioning. In experiments with freshly refrigerated grass carp and defrosted Alaska pollock fillets it was discovered, initially using Fast Protein and Metabolites Liquid Chromatography and the ATP- bioluminescence test, and afterwards validated by NMR spectroscopy, that heat treatment identical to conventional culinary processing in aqueous or wet media at temperatures above 62 ◦C leads to nucleotide salvage (recovery) in aged fish. A significant increase in the concentration of IMP, and even an emergence of ANP substances, were reliably demonstrated in fish samples which had already partially or fully lost these components during prolonged storage due to the ATP breakdown metabolic reactions. Owing to this recovery, the nutritive value of ready-to-eat food can be higher than was initially evaluated in raw products before heat treatment: an effect that should certainly be considered in practical nutrition. Moreover, it is necessary to reconsider the widely acknowledged system of indices of freshness based on nucleotides and nucleosides elaborated a long time ago for raw meat and fish products. This research was partially financially supported by the Estonian Research Council grant (PRG 1441) of the Estonian University of Life Sciences (A.S., P.R., T.P., M.R.). This research was partially financially supported by the Estonian Research Council grant (PRG 1441) of the Estonian University of Life Sciences (A.S., P.R., T.P., M.R.). Article in Journal/Newspaper alaska pollock Alaska Estonian University of Life Sciences: DSpace AppliedChem 3 2 334 349 |
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Estonian University of Life Sciences: DSpace |
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nuclear magnetic resonance fast protein liquid chromatography fish freshness dietary nucleotides nucleotide salvage articles |
spellingShingle |
nuclear magnetic resonance fast protein liquid chromatography fish freshness dietary nucleotides nucleotide salvage articles Sünter, Alar Kuznetsov, Artur Rausepp, Piret Püssa, Tõnu Toom, Lauri Konoplev, Georgii Stepanova, Oksana S. Stepanova, Oksana V. Lyalin, Daniil Frorip, Aleksandr Roasto, Mati Manifestation of Heat-Induced Valuable Dietary Nucleotide Salvage in Food Prepared from Aged Fish in Fast Protein and Metabolites Liquid Chromatography, ATP-Bioluminescence Assay, and NMR Spectra |
topic_facet |
nuclear magnetic resonance fast protein liquid chromatography fish freshness dietary nucleotides nucleotide salvage articles |
description |
Dietary nucleotides and nucleosides, primarily inosine monophosphate (IMP) and the adenine nucleotide pool (ANP), are widely considered as essential nutrients responsible for multiple biological functions. Food prepared from meat and fish is the main source of these substances in the human diet, and it is extremely important to implement storage and processing techniques ensuring their maximum preservation and even accumulation during maturation or conditioning. In experiments with freshly refrigerated grass carp and defrosted Alaska pollock fillets it was discovered, initially using Fast Protein and Metabolites Liquid Chromatography and the ATP- bioluminescence test, and afterwards validated by NMR spectroscopy, that heat treatment identical to conventional culinary processing in aqueous or wet media at temperatures above 62 ◦C leads to nucleotide salvage (recovery) in aged fish. A significant increase in the concentration of IMP, and even an emergence of ANP substances, were reliably demonstrated in fish samples which had already partially or fully lost these components during prolonged storage due to the ATP breakdown metabolic reactions. Owing to this recovery, the nutritive value of ready-to-eat food can be higher than was initially evaluated in raw products before heat treatment: an effect that should certainly be considered in practical nutrition. Moreover, it is necessary to reconsider the widely acknowledged system of indices of freshness based on nucleotides and nucleosides elaborated a long time ago for raw meat and fish products. This research was partially financially supported by the Estonian Research Council grant (PRG 1441) of the Estonian University of Life Sciences (A.S., P.R., T.P., M.R.). This research was partially financially supported by the Estonian Research Council grant (PRG 1441) of the Estonian University of Life Sciences (A.S., P.R., T.P., M.R.). |
format |
Article in Journal/Newspaper |
author |
Sünter, Alar Kuznetsov, Artur Rausepp, Piret Püssa, Tõnu Toom, Lauri Konoplev, Georgii Stepanova, Oksana S. Stepanova, Oksana V. Lyalin, Daniil Frorip, Aleksandr Roasto, Mati |
author_facet |
Sünter, Alar Kuznetsov, Artur Rausepp, Piret Püssa, Tõnu Toom, Lauri Konoplev, Georgii Stepanova, Oksana S. Stepanova, Oksana V. Lyalin, Daniil Frorip, Aleksandr Roasto, Mati |
author_sort |
Sünter, Alar |
title |
Manifestation of Heat-Induced Valuable Dietary Nucleotide Salvage in Food Prepared from Aged Fish in Fast Protein and Metabolites Liquid Chromatography, ATP-Bioluminescence Assay, and NMR Spectra |
title_short |
Manifestation of Heat-Induced Valuable Dietary Nucleotide Salvage in Food Prepared from Aged Fish in Fast Protein and Metabolites Liquid Chromatography, ATP-Bioluminescence Assay, and NMR Spectra |
title_full |
Manifestation of Heat-Induced Valuable Dietary Nucleotide Salvage in Food Prepared from Aged Fish in Fast Protein and Metabolites Liquid Chromatography, ATP-Bioluminescence Assay, and NMR Spectra |
title_fullStr |
Manifestation of Heat-Induced Valuable Dietary Nucleotide Salvage in Food Prepared from Aged Fish in Fast Protein and Metabolites Liquid Chromatography, ATP-Bioluminescence Assay, and NMR Spectra |
title_full_unstemmed |
Manifestation of Heat-Induced Valuable Dietary Nucleotide Salvage in Food Prepared from Aged Fish in Fast Protein and Metabolites Liquid Chromatography, ATP-Bioluminescence Assay, and NMR Spectra |
title_sort |
manifestation of heat-induced valuable dietary nucleotide salvage in food prepared from aged fish in fast protein and metabolites liquid chromatography, atp-bioluminescence assay, and nmr spectra |
publisher |
MDPI |
publishDate |
2023 |
url |
http://hdl.handle.net/10492/8669 https://doi.org/10.3390/appliedchem3020021 |
genre |
alaska pollock Alaska |
genre_facet |
alaska pollock Alaska |
op_relation |
This work was supported by the Estonian Research Council grant (PRG1441). AppliedChem 2023, 3, 334–34 2673-9623 http://hdl.handle.net/10492/8669 https://doi.org/10.3390/appliedchem3020021 Sünter, A.; Kuznetsov, A.; Raudsepp, P.; Püssa, T.; Toom, L.; Konoplev, G.; Stepanova, O.S.; Stepanova, O.V.; Lyalin, D.; Frorip, A.; et al. Manifestation of Heat-Induced Valuable Dietary Nucleotide Salvage in Food Prepared from Aged Fish in Fast Protein and Metabolites Liquid Chromatography, ATP-Bioluminescence Assay, and NMR Spectra. AppliedChem 2023, 3, 334–349. https://doi.org/10.3390/ appliedchem3020021 |
op_rights |
Creative Commons Attribution 4.0 International (CC BY 4.0) © 2023 by the authors. info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/appliedchem3020021 |
container_title |
AppliedChem |
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3 |
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2 |
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334 |
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349 |
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