Identification of enterococcus species isolated from commercial fish feeds and infected fish specimens
The genus of lactic acid bacteria Enterococci are Gram-positive cocci bacterium that can survive in different environmental conditions such as water, plants, and soil. They are also bacteriological signs of fecal contamination. In aquaculture facilities, Enterococcus species have appeared as one of...
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fterdoganuniv:oai:acikerisim.erdogan.edu.tr:11436/9274 2024-09-15T18:40:01+00:00 Identification of enterococcus species isolated from commercial fish feeds and infected fish specimens Kumru, Salih Balta, Z. Dengiz Aliu, H. Balta, F. RTEÜ, Su Ürünleri Fakültesi, Su Ürünleri Yetiştiriciliği Bölümü Kumru, Salih Balta, Z. Dengiz Balta, F. 2024 application/pdf https://hdl.handle.net/11436/9274 https://doi.org/10.12681/jhvms.35199 eng eng Hellenic Veterinary Medical Society Journal of the Hellenic Veterinary Medical Society Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı Kumru, S., Dengiz Balta, Z., Aliu, H., & Balta, F. (2024). Identification of Enterococcus Species Isolated from Commercial Fish Feeds and Infected Fish Specimens. Journal of the Hellenic Veterinary Medical Society, 75(2), 7523–7530. https://doi.org/10.12681/jhvms.35199 1792-2720 https://doi.org/10.12681/jhvms.35199 https://hdl.handle.net/11436/9274 doi:10.12681/jhvms.35199 75 2 7523 7530 info:eu-repo/semantics/openAccess Enterococcus Enterococcus faecalis Enterococcus faecium Fish feeds Trout article 2024 fterdoganuniv https://doi.org/10.12681/jhvms.35199 2024-08-22T23:30:40Z The genus of lactic acid bacteria Enterococci are Gram-positive cocci bacterium that can survive in different environmental conditions such as water, plants, and soil. They are also bacteriological signs of fecal contamination. In aquaculture facilities, Enterococcus species have appeared as one of the crucial opportunistic fish pathogens. Enterococcus-caused fish diseases have been reported in different fish species like yellow tail, turbot, and tilapia. Even though Enterococcus species are used as probiotics and are members of the gastrointestinal flora, they also have pathogenic potential to produce septicemia, wound infections, urinary tract infections, and others. In this study, we isolated bacterial strains from affected rainbow trout and trout feed specimens. Based on the API 20 strep test kit, they were determined as Enterococcus faecium. While fish-isolated samples had 74.4%-99.9% similarity to E. faecium, trout feed isolated samples had 98.4%-99.9% similarity to E. faecium. In order to identify the isolates of the trout feed, PCR was performed using universal 16S rRNA primers. Sequence results indicate that the samples were E. faecium and E. faecalis. The phylogenetic tree was constructed with other Enterococcus species of 16S rRNA, and our samples were located in the E. faecium and E. faecalis species. In conclusion, there may be contamination of Enterococcus with food or other factors. Enterococcus sp. strains are opportunistic microorganisms and cause pathogenicity when the host immunity weakens. Even though all samples with API 20 strep test kit were identified as E. faecium, they had the lower percentage similarity, so they may be E. faecalis and other Enterococcus species. Thus, further studies are needed to understand their probiotic and pathogenicity functions in aquaculture production. Article in Journal/Newspaper Turbot Recep Tayyip Erdoğan University Institutional Repository (DSpace@RTEÜ) |
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Recep Tayyip Erdoğan University Institutional Repository (DSpace@RTEÜ) |
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fterdoganuniv |
language |
English |
topic |
Enterococcus Enterococcus faecalis Enterococcus faecium Fish feeds Trout |
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Enterococcus Enterococcus faecalis Enterococcus faecium Fish feeds Trout Kumru, Salih Balta, Z. Dengiz Aliu, H. Balta, F. Identification of enterococcus species isolated from commercial fish feeds and infected fish specimens |
topic_facet |
Enterococcus Enterococcus faecalis Enterococcus faecium Fish feeds Trout |
description |
The genus of lactic acid bacteria Enterococci are Gram-positive cocci bacterium that can survive in different environmental conditions such as water, plants, and soil. They are also bacteriological signs of fecal contamination. In aquaculture facilities, Enterococcus species have appeared as one of the crucial opportunistic fish pathogens. Enterococcus-caused fish diseases have been reported in different fish species like yellow tail, turbot, and tilapia. Even though Enterococcus species are used as probiotics and are members of the gastrointestinal flora, they also have pathogenic potential to produce septicemia, wound infections, urinary tract infections, and others. In this study, we isolated bacterial strains from affected rainbow trout and trout feed specimens. Based on the API 20 strep test kit, they were determined as Enterococcus faecium. While fish-isolated samples had 74.4%-99.9% similarity to E. faecium, trout feed isolated samples had 98.4%-99.9% similarity to E. faecium. In order to identify the isolates of the trout feed, PCR was performed using universal 16S rRNA primers. Sequence results indicate that the samples were E. faecium and E. faecalis. The phylogenetic tree was constructed with other Enterococcus species of 16S rRNA, and our samples were located in the E. faecium and E. faecalis species. In conclusion, there may be contamination of Enterococcus with food or other factors. Enterococcus sp. strains are opportunistic microorganisms and cause pathogenicity when the host immunity weakens. Even though all samples with API 20 strep test kit were identified as E. faecium, they had the lower percentage similarity, so they may be E. faecalis and other Enterococcus species. Thus, further studies are needed to understand their probiotic and pathogenicity functions in aquaculture production. |
author2 |
RTEÜ, Su Ürünleri Fakültesi, Su Ürünleri Yetiştiriciliği Bölümü Kumru, Salih Balta, Z. Dengiz Balta, F. |
format |
Article in Journal/Newspaper |
author |
Kumru, Salih Balta, Z. Dengiz Aliu, H. Balta, F. |
author_facet |
Kumru, Salih Balta, Z. Dengiz Aliu, H. Balta, F. |
author_sort |
Kumru, Salih |
title |
Identification of enterococcus species isolated from commercial fish feeds and infected fish specimens |
title_short |
Identification of enterococcus species isolated from commercial fish feeds and infected fish specimens |
title_full |
Identification of enterococcus species isolated from commercial fish feeds and infected fish specimens |
title_fullStr |
Identification of enterococcus species isolated from commercial fish feeds and infected fish specimens |
title_full_unstemmed |
Identification of enterococcus species isolated from commercial fish feeds and infected fish specimens |
title_sort |
identification of enterococcus species isolated from commercial fish feeds and infected fish specimens |
publisher |
Hellenic Veterinary Medical Society |
publishDate |
2024 |
url |
https://hdl.handle.net/11436/9274 https://doi.org/10.12681/jhvms.35199 |
genre |
Turbot |
genre_facet |
Turbot |
op_relation |
Journal of the Hellenic Veterinary Medical Society Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı Kumru, S., Dengiz Balta, Z., Aliu, H., & Balta, F. (2024). Identification of Enterococcus Species Isolated from Commercial Fish Feeds and Infected Fish Specimens. Journal of the Hellenic Veterinary Medical Society, 75(2), 7523–7530. https://doi.org/10.12681/jhvms.35199 1792-2720 https://doi.org/10.12681/jhvms.35199 https://hdl.handle.net/11436/9274 doi:10.12681/jhvms.35199 75 2 7523 7530 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.12681/jhvms.35199 |
_version_ |
1810484338620366848 |