EFFECT OF INOCULATION ON THE BIOREMEDIATION OF WEATHERED PRUDHOE BAY CRUDE OIL

• Main Authors: J.G. Mueller, S.M. Resnick, M.E. Shelton, P.H. Pritchard
• Format: Text
• Language: unknown
• Published: 2004
• Subjects: Prudhoe Bay; Alaska
• Online Access: http://oaspub.epa.gov/eims/eimsapi.dispdetail?deid=41968

Enrichment cultures from oil-contaminated beach material from Prince William Sound, Alaska, generated both a mixed bacterial community of indigenous, oil-degrading marine microorganisms and a pure culture oil-degrader, strain E12V. he mixed and axenic cultures were used in comparative shake flask studies of inoculation on biodegradation of Prudhoe Bay crude oil. ithin 12 h following inoculation of homogenized, oiled beach material with the mixed culture, total CO2 production was increased 2-fold relative to a noninoculated control. oreover, measurements of phenanthrene degradation (as determined by the release of 14CO2 from 14C-9-phenanthrene) showed a 2- or 3-fold greater degradation when inoculated with either strain E12V or with the mixed culture, respectively.