PBN11-8, a Cytotoxic Polypeptide Purified from Marine Bacillus, Suppresses Invasion and Migration of Human Hepatocellular Carcinoma Cells by Targeting Focal Adhesion Kinase Pathways
The development of antitumor drugs has attracted cancer researchers and the identification of novel antitumor lead compounds is certainly of great interest. The fermentation broth of Bacillus sp. N11-8, which was isolated from the Antarctic waters, showed cytotoxicity towards different cells. A cyto...
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MDPI AG
2018
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Online Access: | https://scholia.toolforge.org/work/Q92943512 http://www.wikidata.org/entity/Q92943512 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6403900 https://doi.org/10.3390/POLYM10091043 |
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ftenkore:wikidata-Q92943512 2023-10-09T21:45:01+02:00 PBN11-8, a Cytotoxic Polypeptide Purified from Marine Bacillus, Suppresses Invasion and Migration of Human Hepatocellular Carcinoma Cells by Targeting Focal Adhesion Kinase Pathways Zheng, Lanhong Zhu, Xiangjie Yang, Kangli Zhu, Meihong Farooqi, Ammad Ahmad Kang, Daole Sun, Mi Xu, Yixin Lin, Xiukun Feng, Yingang Liang, Fangfang Zhang, Fuming Linhardt, Robert J. 2018-09-19 https://scholia.toolforge.org/work/Q92943512 http://www.wikidata.org/entity/Q92943512 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6403900 https://doi.org/10.3390/POLYM10091043 en eng MDPI AG https://scholia.toolforge.org/work/Q92943512 http://www.wikidata.org/entity/Q92943512 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6403900 doi:10.3390/POLYM10091043 https://creativecommons.org/licenses/by/4.0/ theme:invasion pathway theme:wikidata.org/entity/Q113019190 aquatic invasion invasion pathway journal article 2018 ftenkore https://doi.org/10.3390/POLYM10091043 2023-09-22T09:37:05Z The development of antitumor drugs has attracted cancer researchers and the identification of novel antitumor lead compounds is certainly of great interest. The fermentation broth of Bacillus sp. N11-8, which was isolated from the Antarctic waters, showed cytotoxicity towards different cells. A cytotoxic polypeptide, PBN11-8, was purified from the fermentation broth of Bacillus sp. N11-8 using ultrafiltration, ammonium sulfate precipitation, anion exchange liquid chromatography and high performance liquid chromatography (HPLC). Cloning and sequence analysis showed that PBN11-8 polypeptide (MW: ~19 kDa by the electrospray-ionization (ESI)) displayed high similarity with peptidase M84 from Bacillus pumilus. PBN11-8 possessed moderate cytotoxicity towards several cancer cell lines with IC50 values of 1.56, 1.80, 1.57, and 1.73 _g/mL against human hepatocellular carcinoma cell line BEL-7402, human renal clear cell adenocarcinoma cell line 786-0, human hepatocellular carcinoma cell line HepG2, and human pancreatic cancer cell line Panc-28, respectively. Moreover, the polypeptide displayed weak cytotoxicity towards normal cell line renal tubular epithelial cell line HK2 and human normal liver cell line L02 cells. Wound healing migration and Transwell experiments demonstrate that PBN11-8 could inhibit the migration and invasion of BEL-7402. Further investigation revealed that PBN11-8 suppresses focal adhesion kinase (FAK)-mediated adhesion, migration, and invasion by disturbing FAK/extracellular regulated protein kinases (ERK) signaling and matrix metalloproteinase-2(MMP-2) and matrix metalloproteinase-9 (MMP-9) in BEL-7402 cells. Thus, PBN11-8 represents a potential novel anti-cancer lead compound. Article in Journal/Newspaper Antarc* Antarctic enKORE project Antarctic The Antarctic Polymers 10 9 1043 |
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enKORE project |
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language |
English |
topic |
theme:invasion pathway theme:wikidata.org/entity/Q113019190 aquatic invasion invasion pathway |
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theme:invasion pathway theme:wikidata.org/entity/Q113019190 aquatic invasion invasion pathway Zheng, Lanhong Zhu, Xiangjie Yang, Kangli Zhu, Meihong Farooqi, Ammad Ahmad Kang, Daole Sun, Mi Xu, Yixin Lin, Xiukun Feng, Yingang Liang, Fangfang Zhang, Fuming Linhardt, Robert J. PBN11-8, a Cytotoxic Polypeptide Purified from Marine Bacillus, Suppresses Invasion and Migration of Human Hepatocellular Carcinoma Cells by Targeting Focal Adhesion Kinase Pathways |
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theme:invasion pathway theme:wikidata.org/entity/Q113019190 aquatic invasion invasion pathway |
description |
The development of antitumor drugs has attracted cancer researchers and the identification of novel antitumor lead compounds is certainly of great interest. The fermentation broth of Bacillus sp. N11-8, which was isolated from the Antarctic waters, showed cytotoxicity towards different cells. A cytotoxic polypeptide, PBN11-8, was purified from the fermentation broth of Bacillus sp. N11-8 using ultrafiltration, ammonium sulfate precipitation, anion exchange liquid chromatography and high performance liquid chromatography (HPLC). Cloning and sequence analysis showed that PBN11-8 polypeptide (MW: ~19 kDa by the electrospray-ionization (ESI)) displayed high similarity with peptidase M84 from Bacillus pumilus. PBN11-8 possessed moderate cytotoxicity towards several cancer cell lines with IC50 values of 1.56, 1.80, 1.57, and 1.73 _g/mL against human hepatocellular carcinoma cell line BEL-7402, human renal clear cell adenocarcinoma cell line 786-0, human hepatocellular carcinoma cell line HepG2, and human pancreatic cancer cell line Panc-28, respectively. Moreover, the polypeptide displayed weak cytotoxicity towards normal cell line renal tubular epithelial cell line HK2 and human normal liver cell line L02 cells. Wound healing migration and Transwell experiments demonstrate that PBN11-8 could inhibit the migration and invasion of BEL-7402. Further investigation revealed that PBN11-8 suppresses focal adhesion kinase (FAK)-mediated adhesion, migration, and invasion by disturbing FAK/extracellular regulated protein kinases (ERK) signaling and matrix metalloproteinase-2(MMP-2) and matrix metalloproteinase-9 (MMP-9) in BEL-7402 cells. Thus, PBN11-8 represents a potential novel anti-cancer lead compound. |
format |
Article in Journal/Newspaper |
author |
Zheng, Lanhong Zhu, Xiangjie Yang, Kangli Zhu, Meihong Farooqi, Ammad Ahmad Kang, Daole Sun, Mi Xu, Yixin Lin, Xiukun Feng, Yingang Liang, Fangfang Zhang, Fuming Linhardt, Robert J. |
author_facet |
Zheng, Lanhong Zhu, Xiangjie Yang, Kangli Zhu, Meihong Farooqi, Ammad Ahmad Kang, Daole Sun, Mi Xu, Yixin Lin, Xiukun Feng, Yingang Liang, Fangfang Zhang, Fuming Linhardt, Robert J. |
author_sort |
Zheng, Lanhong |
title |
PBN11-8, a Cytotoxic Polypeptide Purified from Marine Bacillus, Suppresses Invasion and Migration of Human Hepatocellular Carcinoma Cells by Targeting Focal Adhesion Kinase Pathways |
title_short |
PBN11-8, a Cytotoxic Polypeptide Purified from Marine Bacillus, Suppresses Invasion and Migration of Human Hepatocellular Carcinoma Cells by Targeting Focal Adhesion Kinase Pathways |
title_full |
PBN11-8, a Cytotoxic Polypeptide Purified from Marine Bacillus, Suppresses Invasion and Migration of Human Hepatocellular Carcinoma Cells by Targeting Focal Adhesion Kinase Pathways |
title_fullStr |
PBN11-8, a Cytotoxic Polypeptide Purified from Marine Bacillus, Suppresses Invasion and Migration of Human Hepatocellular Carcinoma Cells by Targeting Focal Adhesion Kinase Pathways |
title_full_unstemmed |
PBN11-8, a Cytotoxic Polypeptide Purified from Marine Bacillus, Suppresses Invasion and Migration of Human Hepatocellular Carcinoma Cells by Targeting Focal Adhesion Kinase Pathways |
title_sort |
pbn11-8, a cytotoxic polypeptide purified from marine bacillus, suppresses invasion and migration of human hepatocellular carcinoma cells by targeting focal adhesion kinase pathways |
publisher |
MDPI AG |
publishDate |
2018 |
url |
https://scholia.toolforge.org/work/Q92943512 http://www.wikidata.org/entity/Q92943512 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6403900 https://doi.org/10.3390/POLYM10091043 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
https://scholia.toolforge.org/work/Q92943512 http://www.wikidata.org/entity/Q92943512 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6403900 doi:10.3390/POLYM10091043 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/POLYM10091043 |
container_title |
Polymers |
container_volume |
10 |
container_issue |
9 |
container_start_page |
1043 |
_version_ |
1779315373399605248 |