Hexachlorobenzene exerts genotoxic effects in a humpback whale cell line under stable exposure conditions
Humpback whales, like other polar wildlife, accumulate persistent organic pollutants. In Southern hemisphere populations, hexachlorobenzene (HCB) dominates the contaminant profiles. HCB is linked to a variety of health effects and is classified as a group 2B carcinogen, but the mechanism of action i...
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Royal Society of Chemistry
2019
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Online Access: | https://doi.org/10.1039/C9RA05352B |
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ftempa:oai:dora:empa_20774 2023-05-15T16:35:51+02:00 Hexachlorobenzene exerts genotoxic effects in a humpback whale cell line under stable exposure conditions Maner, Jenny Burkard, Michael Cassano, Juan Carlos Bengtson Nash, Susan M. Schirmer, Kristin Suter, Marc J.-F. 2019 https://doi.org/10.1039/C9RA05352B eng eng Royal Society of Chemistry RSC Advances--RSC Adv.--journals:2534--2046-2069 empa:20774 journal id: journals:2534 issn: 2046-2069 doi:10.1039/C9RA05352B ut: 000501820700055 scopus: 2-s2.0-85076155463 pmid: 35540658 Journal Article Text 2019 ftempa https://doi.org/10.1039/C9RA05352B 2023-03-04T17:14:51Z Humpback whales, like other polar wildlife, accumulate persistent organic pollutants. In Southern hemisphere populations, hexachlorobenzene (HCB) dominates the contaminant profiles. HCB is linked to a variety of health effects and is classified as a group 2B carcinogen, but the mechanism of action is a matter of contention. Potential toxicological effects to humpback whales remain entirely unknown. The recently established humpback whale fibroblast cell line (HuWa) offers an in vitro model for toxicological investigations. We here combine this novel cell line with a passive dosing strategy to investigate whale-specific toxicity of HCB. The relevant partitioning coefficients were determined to produce stable and predictable exposure concentrations in small-scale bioassays. The system was used to assess acute toxicity as well as genotoxicity of HCB to the HuWa cell line. While we found some transient reductions in metabolic activity, measured with the indicator dye alamarBlue, no clear acute toxic effects were discernible. Yet, a significant increase in DNA damage, detected in the alkaline comet assay, was found in HuWa cells exposed to 10 μg L -1 HCB during the sensitive phase of cell attachment. Collectively, this work provides a ready-to-use passive dosing system and delivers evidence that HCB elicits genotoxicity in humpback whale cells. Article in Journal/Newspaper Humpback Whale DORA Empa RSC Advances 9 67 39447 39457 |
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English |
description |
Humpback whales, like other polar wildlife, accumulate persistent organic pollutants. In Southern hemisphere populations, hexachlorobenzene (HCB) dominates the contaminant profiles. HCB is linked to a variety of health effects and is classified as a group 2B carcinogen, but the mechanism of action is a matter of contention. Potential toxicological effects to humpback whales remain entirely unknown. The recently established humpback whale fibroblast cell line (HuWa) offers an in vitro model for toxicological investigations. We here combine this novel cell line with a passive dosing strategy to investigate whale-specific toxicity of HCB. The relevant partitioning coefficients were determined to produce stable and predictable exposure concentrations in small-scale bioassays. The system was used to assess acute toxicity as well as genotoxicity of HCB to the HuWa cell line. While we found some transient reductions in metabolic activity, measured with the indicator dye alamarBlue, no clear acute toxic effects were discernible. Yet, a significant increase in DNA damage, detected in the alkaline comet assay, was found in HuWa cells exposed to 10 μg L -1 HCB during the sensitive phase of cell attachment. Collectively, this work provides a ready-to-use passive dosing system and delivers evidence that HCB elicits genotoxicity in humpback whale cells. |
format |
Article in Journal/Newspaper |
author |
Maner, Jenny Burkard, Michael Cassano, Juan Carlos Bengtson Nash, Susan M. Schirmer, Kristin Suter, Marc J.-F. |
spellingShingle |
Maner, Jenny Burkard, Michael Cassano, Juan Carlos Bengtson Nash, Susan M. Schirmer, Kristin Suter, Marc J.-F. Hexachlorobenzene exerts genotoxic effects in a humpback whale cell line under stable exposure conditions |
author_facet |
Maner, Jenny Burkard, Michael Cassano, Juan Carlos Bengtson Nash, Susan M. Schirmer, Kristin Suter, Marc J.-F. |
author_sort |
Maner, Jenny |
title |
Hexachlorobenzene exerts genotoxic effects in a humpback whale cell line under stable exposure conditions |
title_short |
Hexachlorobenzene exerts genotoxic effects in a humpback whale cell line under stable exposure conditions |
title_full |
Hexachlorobenzene exerts genotoxic effects in a humpback whale cell line under stable exposure conditions |
title_fullStr |
Hexachlorobenzene exerts genotoxic effects in a humpback whale cell line under stable exposure conditions |
title_full_unstemmed |
Hexachlorobenzene exerts genotoxic effects in a humpback whale cell line under stable exposure conditions |
title_sort |
hexachlorobenzene exerts genotoxic effects in a humpback whale cell line under stable exposure conditions |
publisher |
Royal Society of Chemistry |
publishDate |
2019 |
url |
https://doi.org/10.1039/C9RA05352B |
genre |
Humpback Whale |
genre_facet |
Humpback Whale |
op_relation |
RSC Advances--RSC Adv.--journals:2534--2046-2069 empa:20774 journal id: journals:2534 issn: 2046-2069 doi:10.1039/C9RA05352B ut: 000501820700055 scopus: 2-s2.0-85076155463 pmid: 35540658 |
op_doi |
https://doi.org/10.1039/C9RA05352B |
container_title |
RSC Advances |
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9 |
container_issue |
67 |
container_start_page |
39447 |
op_container_end_page |
39457 |
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1766026157693599744 |