Identification of Enterococcus Species Isolated from Commercial Fish Feeds and Infected Fish Specimens
Enterococcus is a Gram‐positive cocci bacterium that can survive in different environmental conditions such as water, plant, and soil. They are also bacteriological signs of fecal contamination. In aquaculture facilities, Enterococcus species have appeared as one of the crucial opportunistic fish pa...
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2024
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ftektojs:oai:ojs.pkp.sfu.ca:article/35199 2024-09-15T18:40:01+00:00 Identification of Enterococcus Species Isolated from Commercial Fish Feeds and Infected Fish Specimens Kumru, S Dengiz Balta, Z Aliu, H Balta, F 2024-07-11 application/pdf https://ejournals.epublishing.ekt.gr/index.php/jhvms/article/view/35199 eng eng Hellenic Veterinary Medical Society / Ελληνική Κτηνιατρική Εταιρεία https://ejournals.epublishing.ekt.gr/index.php/jhvms/article/view/35199/28644 https://ejournals.epublishing.ekt.gr/index.php/jhvms/article/view/35199 Copyright (c) 2024 S Kumru, Z Dengiz Balta, H Aliu, F Balta http://creativecommons.org/licenses/by-nc/4.0 Περιοδικό της Ελληνικής Κτηνιατρικής Εταιρείας; Τόμ. 75 Αρ. 2 (2024); 7523-7530 Journal of the Hellenic Veterinary Medical Society; Vol. 75 No. 2 (2024); 7523-7530 2585-3724 1792-2720 Enterococcus Enterococcus faecalis Enterococcus faecium trout fish feeds info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2024 ftektojs 2024-07-18T03:59:06Z Enterococcus is a Gram‐positive cocci bacterium that can survive in different environmental conditions such as water, plant, and soil. They are also bacteriological signs of fecal contamination. In aquaculture facilities, Enterococcus species have appeared as one of the crucial opportunistic fish pathogens. Enterococcus-caused fish diseases have been reported in different fish species like yellow tail, turbot, and tilapia. Even though Enterococcus species are used as probiotics and are members of the gastrointestinal flora, they can cause pathogenicity like sepsis, wound infections, urinary tract infections, and others. In this study, we isolated bacterial samples from diseased rainbow trout and trout feed. Based on the API 20 strep test kit, they were determined as Enterococcus faecium. While fish isolate samples had 74.4%-99.9% similarity to E. faecium, trout feed isolate samples had 96.4%-99.9% similarity to E. faecium. Moreover, PCR was performed from trout feed isolate samples by using the universal 16S rRNA primers. Sequence results indicate that the samples were E. faecium and E. faecalis. The phylogenetic tree was constructed with other Enterococcus species of 16S rRNA and our samples were located in the E. faecium and E. faecalis species. In conclusion, there may be contamination of Enterococcus with food or other factors. They are opportunistic microorganisms and cause pathogenicity when the host immunity weakens. This may be the first report of Enterococcus contamination and pathogenicity on feeds and fish. Even though all samples with API 20 strep test kit were identified as E. faecium, they had the lower percentage similarity, so they may be E. faecalis and other Enterococcus species. Thus, further studies are needed to understand their probiotic and pathogenicity functions in aquaculture production. Article in Journal/Newspaper Turbot EKT ePublishing (National Documentation Centre, Greece) |
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EKT ePublishing (National Documentation Centre, Greece) |
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English |
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Enterococcus Enterococcus faecalis Enterococcus faecium trout fish feeds |
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Enterococcus Enterococcus faecalis Enterococcus faecium trout fish feeds Kumru, S Dengiz Balta, Z Aliu, H Balta, F Identification of Enterococcus Species Isolated from Commercial Fish Feeds and Infected Fish Specimens |
topic_facet |
Enterococcus Enterococcus faecalis Enterococcus faecium trout fish feeds |
description |
Enterococcus is a Gram‐positive cocci bacterium that can survive in different environmental conditions such as water, plant, and soil. They are also bacteriological signs of fecal contamination. In aquaculture facilities, Enterococcus species have appeared as one of the crucial opportunistic fish pathogens. Enterococcus-caused fish diseases have been reported in different fish species like yellow tail, turbot, and tilapia. Even though Enterococcus species are used as probiotics and are members of the gastrointestinal flora, they can cause pathogenicity like sepsis, wound infections, urinary tract infections, and others. In this study, we isolated bacterial samples from diseased rainbow trout and trout feed. Based on the API 20 strep test kit, they were determined as Enterococcus faecium. While fish isolate samples had 74.4%-99.9% similarity to E. faecium, trout feed isolate samples had 96.4%-99.9% similarity to E. faecium. Moreover, PCR was performed from trout feed isolate samples by using the universal 16S rRNA primers. Sequence results indicate that the samples were E. faecium and E. faecalis. The phylogenetic tree was constructed with other Enterococcus species of 16S rRNA and our samples were located in the E. faecium and E. faecalis species. In conclusion, there may be contamination of Enterococcus with food or other factors. They are opportunistic microorganisms and cause pathogenicity when the host immunity weakens. This may be the first report of Enterococcus contamination and pathogenicity on feeds and fish. Even though all samples with API 20 strep test kit were identified as E. faecium, they had the lower percentage similarity, so they may be E. faecalis and other Enterococcus species. Thus, further studies are needed to understand their probiotic and pathogenicity functions in aquaculture production. |
format |
Article in Journal/Newspaper |
author |
Kumru, S Dengiz Balta, Z Aliu, H Balta, F |
author_facet |
Kumru, S Dengiz Balta, Z Aliu, H Balta, F |
author_sort |
Kumru, S |
title |
Identification of Enterococcus Species Isolated from Commercial Fish Feeds and Infected Fish Specimens |
title_short |
Identification of Enterococcus Species Isolated from Commercial Fish Feeds and Infected Fish Specimens |
title_full |
Identification of Enterococcus Species Isolated from Commercial Fish Feeds and Infected Fish Specimens |
title_fullStr |
Identification of Enterococcus Species Isolated from Commercial Fish Feeds and Infected Fish Specimens |
title_full_unstemmed |
Identification of Enterococcus Species Isolated from Commercial Fish Feeds and Infected Fish Specimens |
title_sort |
identification of enterococcus species isolated from commercial fish feeds and infected fish specimens |
publisher |
Hellenic Veterinary Medical Society / Ελληνική Κτηνιατρική Εταιρεία |
publishDate |
2024 |
url |
https://ejournals.epublishing.ekt.gr/index.php/jhvms/article/view/35199 |
genre |
Turbot |
genre_facet |
Turbot |
op_source |
Περιοδικό της Ελληνικής Κτηνιατρικής Εταιρείας; Τόμ. 75 Αρ. 2 (2024); 7523-7530 Journal of the Hellenic Veterinary Medical Society; Vol. 75 No. 2 (2024); 7523-7530 2585-3724 1792-2720 |
op_relation |
https://ejournals.epublishing.ekt.gr/index.php/jhvms/article/view/35199/28644 https://ejournals.epublishing.ekt.gr/index.php/jhvms/article/view/35199 |
op_rights |
Copyright (c) 2024 S Kumru, Z Dengiz Balta, H Aliu, F Balta http://creativecommons.org/licenses/by-nc/4.0 |
_version_ |
1810484339503267840 |