In situ experiments on the effect of low pH on the ultrastructure of the seagrasses Cymodocea nodosa and Posidonia oceanica
The present study investigates the impacts of low pH on the cell structure of the seagrasses Posidonia oceanica (L.) Delile and Cymodocea nodosa (Ucria) Ascherson. The study was applied with in situ experiments at the Castello Aragonese of Ischia (Naples, Italy), where shallow submarine vents, lower...
Published in: | Mediterranean Marine Science |
---|---|
Main Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Hellenic Centre for Marine Research
2022
|
Subjects: | |
Online Access: | https://ejournals.epublishing.ekt.gr/index.php/hcmr-med-mar-sc/article/view/26314 https://doi.org/10.12681/mms.26314 |
id |
ftektojs:oai:ejournals.epublishing.ekt.gr:article/26314 |
---|---|
record_format |
openpolar |
spelling |
ftektojs:oai:ejournals.epublishing.ekt.gr:article/26314 2023-05-15T17:51:36+02:00 In situ experiments on the effect of low pH on the ultrastructure of the seagrasses Cymodocea nodosa and Posidonia oceanica KOUTALIANOU, MARIA BUIA, MARIA CRISTINA KATSAROS, CHRISTOS Mediterranean Sea 2022-01-28 application/pdf https://ejournals.epublishing.ekt.gr/index.php/hcmr-med-mar-sc/article/view/26314 https://doi.org/10.12681/mms.26314 eng eng Hellenic Centre for Marine Research https://ejournals.epublishing.ekt.gr/index.php/hcmr-med-mar-sc/article/view/26314/22868 https://ejournals.epublishing.ekt.gr/index.php/hcmr-med-mar-sc/article/view/26314/22869 https://ejournals.epublishing.ekt.gr/index.php/hcmr-med-mar-sc/article/view/26314 doi:10.12681/mms.26314 Copyright (c) 2021 Mediterranean Marine Science Mediterranean Marine Science; Vol 23, No 1 (2022); 30-45 1791-6763 1108-393X Plant cell ultrastructure Ocean Acidification Transmission electron microscopy Programmed cell death Microtubules underwater vents info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2022 ftektojs https://doi.org/10.12681/mms.26314 2022-02-03T17:35:11Z The present study investigates the impacts of low pH on the cell structure of the seagrasses Posidonia oceanica (L.) Delile and Cymodocea nodosa (Ucria) Ascherson. The study was applied with in situ experiments at the Castello Aragonese of Ischia (Naples, Italy), where shallow submarine vents, lowering the pH, can be used as natural laboratories. Shoots of the seagrasses were transferred from the control area (pH 8.1) to the two venting areas (pH 7.8 and 6.8) for different times. Epidermal cells of young leaves were examined using transmission electron microscopy (TEM) and tubulin immunofluorescence. After one week at pH 7.8, the cell structure of Posidonia oceanica was normal, while in Cymodocea nodosa microtubule (MT) network and cell structure were affected. In addition, in C. nodosa, ultrastructural analysis revealed a gradual degradation of the nuclei, a disorganization of the chloroplasts, and an increase in the number of mitochondria and dictyosomes. The exposure of both plants for 3 weeks at pH 6.8 resulted in the aggregation and finally in the dilation of the endoplasmic reticulum (ER) membranes. Tubulin immunofluorescence revealed that after three weeks, the MT cytoskeleton of both plants was severely affected. All these alterations can be considered as indications of an apoptotic like programmed cell death (AL-PCD) which may be executed in order to regulate stress response. Article in Journal/Newspaper Ocean acidification EKT ePublishing (National Documentation Centre, Greece) Mediterranean Marine Science |
institution |
Open Polar |
collection |
EKT ePublishing (National Documentation Centre, Greece) |
op_collection_id |
ftektojs |
language |
English |
topic |
Plant cell ultrastructure Ocean Acidification Transmission electron microscopy Programmed cell death Microtubules underwater vents |
spellingShingle |
Plant cell ultrastructure Ocean Acidification Transmission electron microscopy Programmed cell death Microtubules underwater vents KOUTALIANOU, MARIA BUIA, MARIA CRISTINA KATSAROS, CHRISTOS In situ experiments on the effect of low pH on the ultrastructure of the seagrasses Cymodocea nodosa and Posidonia oceanica |
topic_facet |
Plant cell ultrastructure Ocean Acidification Transmission electron microscopy Programmed cell death Microtubules underwater vents |
description |
The present study investigates the impacts of low pH on the cell structure of the seagrasses Posidonia oceanica (L.) Delile and Cymodocea nodosa (Ucria) Ascherson. The study was applied with in situ experiments at the Castello Aragonese of Ischia (Naples, Italy), where shallow submarine vents, lowering the pH, can be used as natural laboratories. Shoots of the seagrasses were transferred from the control area (pH 8.1) to the two venting areas (pH 7.8 and 6.8) for different times. Epidermal cells of young leaves were examined using transmission electron microscopy (TEM) and tubulin immunofluorescence. After one week at pH 7.8, the cell structure of Posidonia oceanica was normal, while in Cymodocea nodosa microtubule (MT) network and cell structure were affected. In addition, in C. nodosa, ultrastructural analysis revealed a gradual degradation of the nuclei, a disorganization of the chloroplasts, and an increase in the number of mitochondria and dictyosomes. The exposure of both plants for 3 weeks at pH 6.8 resulted in the aggregation and finally in the dilation of the endoplasmic reticulum (ER) membranes. Tubulin immunofluorescence revealed that after three weeks, the MT cytoskeleton of both plants was severely affected. All these alterations can be considered as indications of an apoptotic like programmed cell death (AL-PCD) which may be executed in order to regulate stress response. |
format |
Article in Journal/Newspaper |
author |
KOUTALIANOU, MARIA BUIA, MARIA CRISTINA KATSAROS, CHRISTOS |
author_facet |
KOUTALIANOU, MARIA BUIA, MARIA CRISTINA KATSAROS, CHRISTOS |
author_sort |
KOUTALIANOU, MARIA |
title |
In situ experiments on the effect of low pH on the ultrastructure of the seagrasses Cymodocea nodosa and Posidonia oceanica |
title_short |
In situ experiments on the effect of low pH on the ultrastructure of the seagrasses Cymodocea nodosa and Posidonia oceanica |
title_full |
In situ experiments on the effect of low pH on the ultrastructure of the seagrasses Cymodocea nodosa and Posidonia oceanica |
title_fullStr |
In situ experiments on the effect of low pH on the ultrastructure of the seagrasses Cymodocea nodosa and Posidonia oceanica |
title_full_unstemmed |
In situ experiments on the effect of low pH on the ultrastructure of the seagrasses Cymodocea nodosa and Posidonia oceanica |
title_sort |
in situ experiments on the effect of low ph on the ultrastructure of the seagrasses cymodocea nodosa and posidonia oceanica |
publisher |
Hellenic Centre for Marine Research |
publishDate |
2022 |
url |
https://ejournals.epublishing.ekt.gr/index.php/hcmr-med-mar-sc/article/view/26314 https://doi.org/10.12681/mms.26314 |
op_coverage |
Mediterranean Sea |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
Mediterranean Marine Science; Vol 23, No 1 (2022); 30-45 1791-6763 1108-393X |
op_relation |
https://ejournals.epublishing.ekt.gr/index.php/hcmr-med-mar-sc/article/view/26314/22868 https://ejournals.epublishing.ekt.gr/index.php/hcmr-med-mar-sc/article/view/26314/22869 https://ejournals.epublishing.ekt.gr/index.php/hcmr-med-mar-sc/article/view/26314 doi:10.12681/mms.26314 |
op_rights |
Copyright (c) 2021 Mediterranean Marine Science |
op_doi |
https://doi.org/10.12681/mms.26314 |
container_title |
Mediterranean Marine Science |
_version_ |
1766158791985856512 |