An Automated Assay for the Evaluation of Mortality in Fish Embryo
International audience Waterways are often first and severely affected by pollution. In this context, fish embryos – which constitute a good model for sensitivity to chemicals – are widely used in environmental toxicology studies. Such studies are devoted to the analysis of a wide spectrum of physio...
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ftecoleponts:oai:HAL:hal-01154543v1 2024-09-15T18:13:56+00:00 An Automated Assay for the Evaluation of Mortality in Fish Embryo Puybareau, Élodie Léonard, Marc Talbot, Hugues Laboratoire d'Informatique Gaspard-Monge (LIGM) Université Paris-Est Marne-la-Vallée (UPEM)-École des Ponts ParisTech (ENPC)-ESIEE Paris-Fédération de Recherche Bézout (BEZOUT) Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) L’Oréal Recherche & Innovation L'OREAL Reykjavik, Iceland 2015-05-26 https://hal.science/hal-01154543 https://hal.science/hal-01154543/document https://hal.science/hal-01154543/file/paper40.pdf https://doi.org/10.1007/978-3-319-18720-4_10 en eng HAL CCSD info:eu-repo/semantics/altIdentifier/doi/10.1007/978-3-319-18720-4_10 hal-01154543 https://hal.science/hal-01154543 https://hal.science/hal-01154543/document https://hal.science/hal-01154543/file/paper40.pdf doi:10.1007/978-3-319-18720-4_10 info:eu-repo/semantics/OpenAccess ISMM 2015 https://hal.science/hal-01154543 ISMM 2015, May 2015, Reykjavik, Iceland. ⟨10.1007/978-3-319-18720-4_10⟩ Toxicology medaka image stabilisation change detection connected filtering [INFO]Computer Science [cs] info:eu-repo/semantics/conferenceObject Conference papers 2015 ftecoleponts https://doi.org/10.1007/978-3-319-18720-4_10 2024-07-24T07:39:31Z International audience Waterways are often first and severely affected by pollution. In this context, fish embryos – which constitute a good model for sensitivity to chemicals – are widely used in environmental toxicology studies. Such studies are devoted to the analysis of a wide spectrum of physiological parameters, for instance mortality ratio. In this article, we develop an assay to determine the mortality rate of Medaka embryo. Based on video sequences, our purpose is to obtain reliable, repeatable results in a fully automated fashion. To reach that challenging goal, we develop an efficient morphological pipeline that analyses image sequences in a multiscale paradigm, from the global scene to the embryo, and then to its heart, finally analysing its putative motion, characterized by intensity variations. Our pipeline, based on robust morphological operators, has a low computational cost, and was experimentally assessed on a dataset consisting of 660 images, providing a success ratio higher than 99%. Conference Object Iceland École des Ponts ParisTech: HAL 110 121 |
institution |
Open Polar |
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École des Ponts ParisTech: HAL |
op_collection_id |
ftecoleponts |
language |
English |
topic |
Toxicology medaka image stabilisation change detection connected filtering [INFO]Computer Science [cs] |
spellingShingle |
Toxicology medaka image stabilisation change detection connected filtering [INFO]Computer Science [cs] Puybareau, Élodie Léonard, Marc Talbot, Hugues An Automated Assay for the Evaluation of Mortality in Fish Embryo |
topic_facet |
Toxicology medaka image stabilisation change detection connected filtering [INFO]Computer Science [cs] |
description |
International audience Waterways are often first and severely affected by pollution. In this context, fish embryos – which constitute a good model for sensitivity to chemicals – are widely used in environmental toxicology studies. Such studies are devoted to the analysis of a wide spectrum of physiological parameters, for instance mortality ratio. In this article, we develop an assay to determine the mortality rate of Medaka embryo. Based on video sequences, our purpose is to obtain reliable, repeatable results in a fully automated fashion. To reach that challenging goal, we develop an efficient morphological pipeline that analyses image sequences in a multiscale paradigm, from the global scene to the embryo, and then to its heart, finally analysing its putative motion, characterized by intensity variations. Our pipeline, based on robust morphological operators, has a low computational cost, and was experimentally assessed on a dataset consisting of 660 images, providing a success ratio higher than 99%. |
author2 |
Laboratoire d'Informatique Gaspard-Monge (LIGM) Université Paris-Est Marne-la-Vallée (UPEM)-École des Ponts ParisTech (ENPC)-ESIEE Paris-Fédération de Recherche Bézout (BEZOUT) Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) L’Oréal Recherche & Innovation L'OREAL |
format |
Conference Object |
author |
Puybareau, Élodie Léonard, Marc Talbot, Hugues |
author_facet |
Puybareau, Élodie Léonard, Marc Talbot, Hugues |
author_sort |
Puybareau, Élodie |
title |
An Automated Assay for the Evaluation of Mortality in Fish Embryo |
title_short |
An Automated Assay for the Evaluation of Mortality in Fish Embryo |
title_full |
An Automated Assay for the Evaluation of Mortality in Fish Embryo |
title_fullStr |
An Automated Assay for the Evaluation of Mortality in Fish Embryo |
title_full_unstemmed |
An Automated Assay for the Evaluation of Mortality in Fish Embryo |
title_sort |
automated assay for the evaluation of mortality in fish embryo |
publisher |
HAL CCSD |
publishDate |
2015 |
url |
https://hal.science/hal-01154543 https://hal.science/hal-01154543/document https://hal.science/hal-01154543/file/paper40.pdf https://doi.org/10.1007/978-3-319-18720-4_10 |
op_coverage |
Reykjavik, Iceland |
genre |
Iceland |
genre_facet |
Iceland |
op_source |
ISMM 2015 https://hal.science/hal-01154543 ISMM 2015, May 2015, Reykjavik, Iceland. ⟨10.1007/978-3-319-18720-4_10⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1007/978-3-319-18720-4_10 hal-01154543 https://hal.science/hal-01154543 https://hal.science/hal-01154543/document https://hal.science/hal-01154543/file/paper40.pdf doi:10.1007/978-3-319-18720-4_10 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1007/978-3-319-18720-4_10 |
container_start_page |
110 |
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121 |
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1810451724048007168 |