Study of the marine pollution by hydrocarbons and characterization of their biochemical and molecular effects on clam Ruditapes sp.
Firstly, biomonitoring of Tunis lagoon was conducted during one year using an approach combining chemicals and biological analyses and the bioindicator species Ruditapesdecussatus. This approach high lighted the high contamination of the Tunis lagoon by tracemetals and polycyclic aromatic hydrocarbo...
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Other Authors: | , , , , , , , |
Format: | Doctoral or Postdoctoral Thesis |
Language: | French |
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HAL CCSD
2015
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Online Access: | https://theses.hal.science/tel-01583022 https://theses.hal.science/tel-01583022/document https://theses.hal.science/tel-01583022/file/CHALGHMI_HOUSSEM_2015.pdf |
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ftecolephe:oai:HAL:tel-01583022v1 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
EPHE (Ecole pratique des hautes études, Paris): HAL |
op_collection_id |
ftecolephe |
language |
French |
topic |
Tunis lagoon Bivalves PAHs Metals Benzo(a)pyrene Biochemical biomarkers Histology Transcriptomic response Genotoxicity Lagune de Tunis HAPs Métaux Benzo(a)pyrène Marqueurs biochimiques Histologie Réponse transcriptomique Génotoxicité [SDU.STU.GC]Sciences of the Universe [physics]/Earth Sciences/Geochemistry [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology |
spellingShingle |
Tunis lagoon Bivalves PAHs Metals Benzo(a)pyrene Biochemical biomarkers Histology Transcriptomic response Genotoxicity Lagune de Tunis HAPs Métaux Benzo(a)pyrène Marqueurs biochimiques Histologie Réponse transcriptomique Génotoxicité [SDU.STU.GC]Sciences of the Universe [physics]/Earth Sciences/Geochemistry [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology Chalghmi, Houssem Study of the marine pollution by hydrocarbons and characterization of their biochemical and molecular effects on clam Ruditapes sp. |
topic_facet |
Tunis lagoon Bivalves PAHs Metals Benzo(a)pyrene Biochemical biomarkers Histology Transcriptomic response Genotoxicity Lagune de Tunis HAPs Métaux Benzo(a)pyrène Marqueurs biochimiques Histologie Réponse transcriptomique Génotoxicité [SDU.STU.GC]Sciences of the Universe [physics]/Earth Sciences/Geochemistry [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology |
description |
Firstly, biomonitoring of Tunis lagoon was conducted during one year using an approach combining chemicals and biological analyses and the bioindicator species Ruditapesdecussatus. This approach high lighted the high contamination of the Tunis lagoon by tracemetals and polycyclic aromatic hydrocarbons (PAHs). Seasonal variation of contaminant bioaccumulation levels in clam was found to be strongly associated with physico chemical changes in surrounding environment and physiological processes in organism. Biological responses investigated through a battery of exposure and effect biomarkers located at differentlevels of biological organisation: molecular (expression of five genes), biochemical(benzo(a)pyrene hydroxylase, glutathione S-transferase, acetylcholinesterase and catalase activities and malondialdehyde concentration) and tissular (histopathological damages),demonstrated a defense process modulation by pollution and showed histopathological alterations in gills and digestive gland involving severe impact of contaminants on health stateof clam. The study of spatio temporal interactions between abiotic and biotic factors identified temperature and reproduction as main parameters affecting defense and effect biochemical response. The principal component analysis (PCA), using all analyzed parameters during thespring, allowed to identify site Z2 as the most affected by pollution. Secondly,benzo(a)pyrene (BaP), already identified in Tunis lagoon, was used in controlled conditions at the laboratory in order to characterize the molecular and biochemical responses in bivalves(Ruditapes philippinarum and Crassostrea gigas) facing a subacute and acute exposures tothis contaminant. This study showed a modulation of biochemical biomarkers of metabolism,oxidative stress and neurotoxicity and expression of genes involved in process of metabolisation, mitochondrial respiration, antioxidant defense, reproduction and immune defense. The analysis of DNA damages revealed a high and early genotoxicity effect of BaPin both ... |
author2 |
Environnements et Paléoenvironnements OCéaniques (EPOC) Observatoire aquitain des sciences de l'univers (OASU) Université Sciences et Technologies - Bordeaux 1 (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1 (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-École Pratique des Hautes Études (EPHE) Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Centre National de la Recherche Scientifique (CNRS) Université de Bordeaux Université de Monastir (Tunisie) Jean-Paul Bourdineaud Dalila Saidane-Mosbahi |
format |
Doctoral or Postdoctoral Thesis |
author |
Chalghmi, Houssem |
author_facet |
Chalghmi, Houssem |
author_sort |
Chalghmi, Houssem |
title |
Study of the marine pollution by hydrocarbons and characterization of their biochemical and molecular effects on clam Ruditapes sp. |
title_short |
Study of the marine pollution by hydrocarbons and characterization of their biochemical and molecular effects on clam Ruditapes sp. |
title_full |
Study of the marine pollution by hydrocarbons and characterization of their biochemical and molecular effects on clam Ruditapes sp. |
title_fullStr |
Study of the marine pollution by hydrocarbons and characterization of their biochemical and molecular effects on clam Ruditapes sp. |
title_full_unstemmed |
Study of the marine pollution by hydrocarbons and characterization of their biochemical and molecular effects on clam Ruditapes sp. |
title_sort |
study of the marine pollution by hydrocarbons and characterization of their biochemical and molecular effects on clam ruditapes sp. |
publisher |
HAL CCSD |
publishDate |
2015 |
url |
https://theses.hal.science/tel-01583022 https://theses.hal.science/tel-01583022/document https://theses.hal.science/tel-01583022/file/CHALGHMI_HOUSSEM_2015.pdf |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
https://theses.hal.science/tel-01583022 Géochimie. Université de Bordeaux; Université de Monastir (Tunisie), 2015. Français. ⟨NNT : 2015BORD0175⟩ |
op_relation |
NNT: 2015BORD0175 tel-01583022 https://theses.hal.science/tel-01583022 https://theses.hal.science/tel-01583022/document https://theses.hal.science/tel-01583022/file/CHALGHMI_HOUSSEM_2015.pdf |
op_rights |
info:eu-repo/semantics/OpenAccess |
_version_ |
1799478910193238016 |
spelling |
ftecolephe:oai:HAL:tel-01583022v1 2024-05-19T07:39:21+00:00 Study of the marine pollution by hydrocarbons and characterization of their biochemical and molecular effects on clam Ruditapes sp. Etude de la pollution marine par les hydrocarbures et caractérisation de leurs effets biochimiques et moléculaires sur la palourde de Ruditapes sp. Chalghmi, Houssem Environnements et Paléoenvironnements OCéaniques (EPOC) Observatoire aquitain des sciences de l'univers (OASU) Université Sciences et Technologies - Bordeaux 1 (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1 (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-École Pratique des Hautes Études (EPHE) Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Centre National de la Recherche Scientifique (CNRS) Université de Bordeaux Université de Monastir (Tunisie) Jean-Paul Bourdineaud Dalila Saidane-Mosbahi 2015-10-06 https://theses.hal.science/tel-01583022 https://theses.hal.science/tel-01583022/document https://theses.hal.science/tel-01583022/file/CHALGHMI_HOUSSEM_2015.pdf fr fre HAL CCSD NNT: 2015BORD0175 tel-01583022 https://theses.hal.science/tel-01583022 https://theses.hal.science/tel-01583022/document https://theses.hal.science/tel-01583022/file/CHALGHMI_HOUSSEM_2015.pdf info:eu-repo/semantics/OpenAccess https://theses.hal.science/tel-01583022 Géochimie. Université de Bordeaux; Université de Monastir (Tunisie), 2015. Français. ⟨NNT : 2015BORD0175⟩ Tunis lagoon Bivalves PAHs Metals Benzo(a)pyrene Biochemical biomarkers Histology Transcriptomic response Genotoxicity Lagune de Tunis HAPs Métaux Benzo(a)pyrène Marqueurs biochimiques Histologie Réponse transcriptomique Génotoxicité [SDU.STU.GC]Sciences of the Universe [physics]/Earth Sciences/Geochemistry [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology info:eu-repo/semantics/doctoralThesis Theses 2015 ftecolephe 2024-04-25T01:55:24Z Firstly, biomonitoring of Tunis lagoon was conducted during one year using an approach combining chemicals and biological analyses and the bioindicator species Ruditapesdecussatus. This approach high lighted the high contamination of the Tunis lagoon by tracemetals and polycyclic aromatic hydrocarbons (PAHs). Seasonal variation of contaminant bioaccumulation levels in clam was found to be strongly associated with physico chemical changes in surrounding environment and physiological processes in organism. Biological responses investigated through a battery of exposure and effect biomarkers located at differentlevels of biological organisation: molecular (expression of five genes), biochemical(benzo(a)pyrene hydroxylase, glutathione S-transferase, acetylcholinesterase and catalase activities and malondialdehyde concentration) and tissular (histopathological damages),demonstrated a defense process modulation by pollution and showed histopathological alterations in gills and digestive gland involving severe impact of contaminants on health stateof clam. The study of spatio temporal interactions between abiotic and biotic factors identified temperature and reproduction as main parameters affecting defense and effect biochemical response. The principal component analysis (PCA), using all analyzed parameters during thespring, allowed to identify site Z2 as the most affected by pollution. Secondly,benzo(a)pyrene (BaP), already identified in Tunis lagoon, was used in controlled conditions at the laboratory in order to characterize the molecular and biochemical responses in bivalves(Ruditapes philippinarum and Crassostrea gigas) facing a subacute and acute exposures tothis contaminant. This study showed a modulation of biochemical biomarkers of metabolism,oxidative stress and neurotoxicity and expression of genes involved in process of metabolisation, mitochondrial respiration, antioxidant defense, reproduction and immune defense. The analysis of DNA damages revealed a high and early genotoxicity effect of BaPin both ... Doctoral or Postdoctoral Thesis Crassostrea gigas EPHE (Ecole pratique des hautes études, Paris): HAL |