Selective upregulation of lipid metabolism in skeletal muscle of foraging juvenile king penguins: an integrative study
International audience The passage from shore to marine life of juvenile penguins represents a major energetic challenge to fuel intense and prolonged demands for thermoregulation and locomotion. Some functional changes developed at this crucial step were investigated by comparing pre-fledging king...
Published in: | Proceedings of the Royal Society B: Biological Sciences |
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Main Authors: | , , , , , , , , , |
Other Authors: | , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2012
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Subjects: | |
Online Access: | https://hal.science/halsde-00707322 https://doi.org/10.1098/Rspb.2011.2664 |
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ftecolephe:oai:HAL:halsde-00707322v1 |
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openpolar |
institution |
Open Polar |
collection |
EPHE (Ecole pratique des hautes études, Paris): HAL |
op_collection_id |
ftecolephe |
language |
English |
topic |
in vivo mitochondria energy substrates transcriptomic analysis [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
spellingShingle |
in vivo mitochondria energy substrates transcriptomic analysis [SDE.BE]Environmental Sciences/Biodiversity and Ecology Teulier, Loïc Dégletagne, Cyril Rey, Benjamin Tornos, Jérémy Keime, Céline de Dinechin, Marc Raccurt, Mireille Rouanet, Damien Roussel, Damien Duchamp, Claude Selective upregulation of lipid metabolism in skeletal muscle of foraging juvenile king penguins: an integrative study |
topic_facet |
in vivo mitochondria energy substrates transcriptomic analysis [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
description |
International audience The passage from shore to marine life of juvenile penguins represents a major energetic challenge to fuel intense and prolonged demands for thermoregulation and locomotion. Some functional changes developed at this crucial step were investigated by comparing pre-fledging king penguins with sea-acclimatized (SA) juveniles (Aptenodytes patagonicus). Transcriptomic analysis of pectoralis muscle biopsies revealed that most genes encoding proteins involved in lipid transport or catabolism were up-regulated, while genes involved in carbohydrate metabolism were mostly downregulated in SA birds. Determination of muscle enzymatic activities showed no changes in enzymes involved in the glycolytic pathway, but increased 3-hydroxyacyl-CoA dehydrogenase, an enzyme of the beta-oxidation pathway. The respiratory rates of isolated muscle mitochondria were much higher with a substrate arising from lipid metabolism (palmitoyl-L-carnitine) in SA juveniles than in terrestrial controls, while no difference emerged with a substrate arising from carbohydrate metabolism (pyruvate). In vivo, perfusion of a lipid emulsion induced a fourfold larger thermogenic effect in SA than in control juveniles. The present integrative study shows that fuel selection towards lipid oxidation characterizes penguin acclimatization to marine life. Such acclimatization may involve thyroid hormones through their nuclear beta receptor and nuclear coactivators. |
author2 |
Équipe 4 - Écophysiologie, Comportement, Conservation (E2C) Laboratoire d'Ecologie des Hydrosystèmes Naturels et Anthropisés (LEHNA) Université Claude Bernard Lyon 1 (UCBL) Université de Lyon-Université de Lyon-École Nationale des Travaux Publics de l'État (ENTPE)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL) Université de Lyon-Université de Lyon-École Nationale des Travaux Publics de l'État (ENTPE)-Centre National de la Recherche Scientifique (CNRS) Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE) Université de Lyon-Université de Lyon-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS) Institute of Genetics Illkirch Molecular and Cellular Biology Centre d’Ecologie Fonctionnelle et Evolutive (CEFE) Université Paul-Valéry - Montpellier 3 (UPVM)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-École Pratique des Hautes Études (EPHE) Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD France-Sud )-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) |
format |
Article in Journal/Newspaper |
author |
Teulier, Loïc Dégletagne, Cyril Rey, Benjamin Tornos, Jérémy Keime, Céline de Dinechin, Marc Raccurt, Mireille Rouanet, Damien Roussel, Damien Duchamp, Claude |
author_facet |
Teulier, Loïc Dégletagne, Cyril Rey, Benjamin Tornos, Jérémy Keime, Céline de Dinechin, Marc Raccurt, Mireille Rouanet, Damien Roussel, Damien Duchamp, Claude |
author_sort |
Teulier, Loïc |
title |
Selective upregulation of lipid metabolism in skeletal muscle of foraging juvenile king penguins: an integrative study |
title_short |
Selective upregulation of lipid metabolism in skeletal muscle of foraging juvenile king penguins: an integrative study |
title_full |
Selective upregulation of lipid metabolism in skeletal muscle of foraging juvenile king penguins: an integrative study |
title_fullStr |
Selective upregulation of lipid metabolism in skeletal muscle of foraging juvenile king penguins: an integrative study |
title_full_unstemmed |
Selective upregulation of lipid metabolism in skeletal muscle of foraging juvenile king penguins: an integrative study |
title_sort |
selective upregulation of lipid metabolism in skeletal muscle of foraging juvenile king penguins: an integrative study |
publisher |
HAL CCSD |
publishDate |
2012 |
url |
https://hal.science/halsde-00707322 https://doi.org/10.1098/Rspb.2011.2664 |
genre |
King Penguins |
genre_facet |
King Penguins |
op_source |
ISSN: 0962-8452 EISSN: 1471-2954 Proceedings of the Royal Society B: Biological Sciences https://hal.science/halsde-00707322 Proceedings of the Royal Society B: Biological Sciences, 2012, 279 (1737), pp.2464-2472. ⟨10.1098/Rspb.2011.2664⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1098/Rspb.2011.2664 halsde-00707322 https://hal.science/halsde-00707322 doi:10.1098/Rspb.2011.2664 PUBMEDCENTRAL: PMC3350679 |
op_doi |
https://doi.org/10.1098/Rspb.2011.2664 |
container_title |
Proceedings of the Royal Society B: Biological Sciences |
container_volume |
279 |
container_issue |
1737 |
container_start_page |
2464 |
op_container_end_page |
2472 |
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1802646528858259456 |
spelling |
ftecolephe:oai:HAL:halsde-00707322v1 2024-06-23T07:54:23+00:00 Selective upregulation of lipid metabolism in skeletal muscle of foraging juvenile king penguins: an integrative study Teulier, Loïc Dégletagne, Cyril Rey, Benjamin Tornos, Jérémy Keime, Céline de Dinechin, Marc Raccurt, Mireille Rouanet, Damien Roussel, Damien Duchamp, Claude Équipe 4 - Écophysiologie, Comportement, Conservation (E2C) Laboratoire d'Ecologie des Hydrosystèmes Naturels et Anthropisés (LEHNA) Université Claude Bernard Lyon 1 (UCBL) Université de Lyon-Université de Lyon-École Nationale des Travaux Publics de l'État (ENTPE)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL) Université de Lyon-Université de Lyon-École Nationale des Travaux Publics de l'État (ENTPE)-Centre National de la Recherche Scientifique (CNRS) Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE) Université de Lyon-Université de Lyon-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS) Institute of Genetics Illkirch Molecular and Cellular Biology Centre d’Ecologie Fonctionnelle et Evolutive (CEFE) Université Paul-Valéry - Montpellier 3 (UPVM)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-École Pratique des Hautes Études (EPHE) Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD France-Sud )-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) 2012 https://hal.science/halsde-00707322 https://doi.org/10.1098/Rspb.2011.2664 en eng HAL CCSD Royal Society, The info:eu-repo/semantics/altIdentifier/doi/10.1098/Rspb.2011.2664 halsde-00707322 https://hal.science/halsde-00707322 doi:10.1098/Rspb.2011.2664 PUBMEDCENTRAL: PMC3350679 ISSN: 0962-8452 EISSN: 1471-2954 Proceedings of the Royal Society B: Biological Sciences https://hal.science/halsde-00707322 Proceedings of the Royal Society B: Biological Sciences, 2012, 279 (1737), pp.2464-2472. ⟨10.1098/Rspb.2011.2664⟩ in vivo mitochondria energy substrates transcriptomic analysis [SDE.BE]Environmental Sciences/Biodiversity and Ecology info:eu-repo/semantics/article Journal articles 2012 ftecolephe https://doi.org/10.1098/Rspb.2011.2664 2024-05-27T23:58:04Z International audience The passage from shore to marine life of juvenile penguins represents a major energetic challenge to fuel intense and prolonged demands for thermoregulation and locomotion. Some functional changes developed at this crucial step were investigated by comparing pre-fledging king penguins with sea-acclimatized (SA) juveniles (Aptenodytes patagonicus). Transcriptomic analysis of pectoralis muscle biopsies revealed that most genes encoding proteins involved in lipid transport or catabolism were up-regulated, while genes involved in carbohydrate metabolism were mostly downregulated in SA birds. Determination of muscle enzymatic activities showed no changes in enzymes involved in the glycolytic pathway, but increased 3-hydroxyacyl-CoA dehydrogenase, an enzyme of the beta-oxidation pathway. The respiratory rates of isolated muscle mitochondria were much higher with a substrate arising from lipid metabolism (palmitoyl-L-carnitine) in SA juveniles than in terrestrial controls, while no difference emerged with a substrate arising from carbohydrate metabolism (pyruvate). In vivo, perfusion of a lipid emulsion induced a fourfold larger thermogenic effect in SA than in control juveniles. The present integrative study shows that fuel selection towards lipid oxidation characterizes penguin acclimatization to marine life. Such acclimatization may involve thyroid hormones through their nuclear beta receptor and nuclear coactivators. Article in Journal/Newspaper King Penguins EPHE (Ecole pratique des hautes études, Paris): HAL Proceedings of the Royal Society B: Biological Sciences 279 1737 2464 2472 |