Comparative responses of sperm cells and embryos of Pacific oyster (Crassostrea gigas) to exposure to metolachlor and its degradation products
International audience Metolachlor is one of the most intensively used chloroacetanilide herbicides in agriculture. Consequently, it has been frequently detected in coastal waters as well as its major degradation products, metolachlor ethane sulfonic acid (MESA) and metolachlor oxanilic acid (MOA) w...
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ftecolephe:oai:HAL:hal-04548088v1 2024-05-19T07:39:16+00:00 Comparative responses of sperm cells and embryos of Pacific oyster (Crassostrea gigas) to exposure to metolachlor and its degradation products Mai, Huong Gonzalez, Patrice Pardon, Patrick Tapie, Nathalie Budzinski, Hélène Cachot, Jérôme Morin, Benedicte Environnements et Paléoenvironnements OCéaniques (EPOC) Observatoire aquitain des sciences de l'univers (OASU) Université Sciences et Technologies - Bordeaux 1 (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1 (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-École Pratique des Hautes Études (EPHE) Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Centre National de la Recherche Scientifique (CNRS) 2013-12-07 https://hal.science/hal-04548088 https://doi.org/10.1016/j.aquatox.2013.11.024 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquatox.2013.11.024 hal-04548088 https://hal.science/hal-04548088 doi:10.1016/j.aquatox.2013.11.024 ISSN: 0166-445X Aquatic Toxicology https://hal.science/hal-04548088 Aquatic Toxicology, 2013, 147, pp.48-56. ⟨10.1016/j.aquatox.2013.11.024⟩ [SDE]Environmental Sciences info:eu-repo/semantics/article Journal articles 2013 ftecolephe https://doi.org/10.1016/j.aquatox.2013.11.024 2024-04-25T00:54:42Z International audience Metolachlor is one of the most intensively used chloroacetanilide herbicides in agriculture. Consequently, it has been frequently detected in coastal waters as well as its major degradation products, metolachlor ethane sulfonic acid (MESA) and metolachlor oxanilic acid (MOA) which are encountered at higher concentrations than metolachlor. Although a few studies of metolachlor toxicity have been conducted on marine organisms, little is known about the environmental toxicity of metolachlor degradation products. In this study, the deleterious effects of metolachlor and its degradation products on spermatozoa and embryos of Crassostrea gigas have been compared using biomarkers of developmental defects, DNA damage and gene transcription levels. After 24h exposure, significant increases in the percentage of abnormal D-larvae and DNA damage were observed from 0.01μgL(-1) for S-metolachlor and 0.1μgL(-1) for MESA and MOA. Results showed that S-metolachlor was more embryotoxic and genotoxic than its degradation products. Oyster sperm was also very sensitive to metolachlor exposure and followed the pattern: metolachlor (0.01μgL(-1))>MOA (0.1μgL(-1))>MESA (1μgL(-1)). Metolachlor and MESA mainly triggered variations in the transcription level of genes encoding proteins involved in oxidative stress responses (mitochondrial superoxide dismutase and catalase). Overall, no significant variation in transcription levels could be detected in C. gigas embryos exposed to MOA. This study demonstrates that metolachlor and its main degradation products have the potential to impact several steps of oyster development and therefore recruitment in coastal areas exposed to chronic inputs of pesticides. Article in Journal/Newspaper Crassostrea gigas Pacific oyster EPHE (Ecole pratique des hautes études, Paris): HAL Aquatic Toxicology 147 48 56 |
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EPHE (Ecole pratique des hautes études, Paris): HAL |
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ftecolephe |
language |
English |
topic |
[SDE]Environmental Sciences |
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[SDE]Environmental Sciences Mai, Huong Gonzalez, Patrice Pardon, Patrick Tapie, Nathalie Budzinski, Hélène Cachot, Jérôme Morin, Benedicte Comparative responses of sperm cells and embryos of Pacific oyster (Crassostrea gigas) to exposure to metolachlor and its degradation products |
topic_facet |
[SDE]Environmental Sciences |
description |
International audience Metolachlor is one of the most intensively used chloroacetanilide herbicides in agriculture. Consequently, it has been frequently detected in coastal waters as well as its major degradation products, metolachlor ethane sulfonic acid (MESA) and metolachlor oxanilic acid (MOA) which are encountered at higher concentrations than metolachlor. Although a few studies of metolachlor toxicity have been conducted on marine organisms, little is known about the environmental toxicity of metolachlor degradation products. In this study, the deleterious effects of metolachlor and its degradation products on spermatozoa and embryos of Crassostrea gigas have been compared using biomarkers of developmental defects, DNA damage and gene transcription levels. After 24h exposure, significant increases in the percentage of abnormal D-larvae and DNA damage were observed from 0.01μgL(-1) for S-metolachlor and 0.1μgL(-1) for MESA and MOA. Results showed that S-metolachlor was more embryotoxic and genotoxic than its degradation products. Oyster sperm was also very sensitive to metolachlor exposure and followed the pattern: metolachlor (0.01μgL(-1))>MOA (0.1μgL(-1))>MESA (1μgL(-1)). Metolachlor and MESA mainly triggered variations in the transcription level of genes encoding proteins involved in oxidative stress responses (mitochondrial superoxide dismutase and catalase). Overall, no significant variation in transcription levels could be detected in C. gigas embryos exposed to MOA. This study demonstrates that metolachlor and its main degradation products have the potential to impact several steps of oyster development and therefore recruitment in coastal areas exposed to chronic inputs of pesticides. |
author2 |
Environnements et Paléoenvironnements OCéaniques (EPOC) Observatoire aquitain des sciences de l'univers (OASU) Université Sciences et Technologies - Bordeaux 1 (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1 (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-École Pratique des Hautes Études (EPHE) Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Mai, Huong Gonzalez, Patrice Pardon, Patrick Tapie, Nathalie Budzinski, Hélène Cachot, Jérôme Morin, Benedicte |
author_facet |
Mai, Huong Gonzalez, Patrice Pardon, Patrick Tapie, Nathalie Budzinski, Hélène Cachot, Jérôme Morin, Benedicte |
author_sort |
Mai, Huong |
title |
Comparative responses of sperm cells and embryos of Pacific oyster (Crassostrea gigas) to exposure to metolachlor and its degradation products |
title_short |
Comparative responses of sperm cells and embryos of Pacific oyster (Crassostrea gigas) to exposure to metolachlor and its degradation products |
title_full |
Comparative responses of sperm cells and embryos of Pacific oyster (Crassostrea gigas) to exposure to metolachlor and its degradation products |
title_fullStr |
Comparative responses of sperm cells and embryos of Pacific oyster (Crassostrea gigas) to exposure to metolachlor and its degradation products |
title_full_unstemmed |
Comparative responses of sperm cells and embryos of Pacific oyster (Crassostrea gigas) to exposure to metolachlor and its degradation products |
title_sort |
comparative responses of sperm cells and embryos of pacific oyster (crassostrea gigas) to exposure to metolachlor and its degradation products |
publisher |
HAL CCSD |
publishDate |
2013 |
url |
https://hal.science/hal-04548088 https://doi.org/10.1016/j.aquatox.2013.11.024 |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 0166-445X Aquatic Toxicology https://hal.science/hal-04548088 Aquatic Toxicology, 2013, 147, pp.48-56. ⟨10.1016/j.aquatox.2013.11.024⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquatox.2013.11.024 hal-04548088 https://hal.science/hal-04548088 doi:10.1016/j.aquatox.2013.11.024 |
op_doi |
https://doi.org/10.1016/j.aquatox.2013.11.024 |
container_title |
Aquatic Toxicology |
container_volume |
147 |
container_start_page |
48 |
op_container_end_page |
56 |
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1799478824254046208 |