Gonadal transcriptome analysis of wild contaminated female European eels during artificial gonad maturation
[Departement_IRSTEA]Eaux [TR1_IRSTEA]QUASARE International audience Since the early 1980s, the population of European eels (Anguilla anguilla) has dramatically declined. Nowadays, the European eel is listed on the red list of threatened species (IUCN Red List) and is considered as critically endange...
Published in: | Chemosphere |
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Main Authors: | , , , , , , , , , , , , |
Other Authors: | , , , , , , , , , , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2015
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Subjects: | |
Online Access: | https://hal.science/hal-02289130 https://doi.org/10.1016/j.chemosphere.2015.06.007 |
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English |
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[SDE]Environmental Sciences |
spellingShingle |
[SDE]Environmental Sciences Dufour, S. Bernatchez, L. Baudrimont, M. Baillon, L. Oses, J. Pierron, F. Colombier, S., Bureau Du Caron, A. Normandeau, E. Lambert, Patrick Couture, P. Labadie, P. Budzinski, H. Gonadal transcriptome analysis of wild contaminated female European eels during artificial gonad maturation |
topic_facet |
[SDE]Environmental Sciences |
description |
[Departement_IRSTEA]Eaux [TR1_IRSTEA]QUASARE International audience Since the early 1980s, the population of European eels (Anguilla anguilla) has dramatically declined. Nowadays, the European eel is listed on the red list of threatened species (IUCN Red List) and is considered as critically endangered of extinction. Pollution is one of the putative causes for the collapse of this species. Among their possible effects, contaminants gradually accumulated in eels during their somatic growth phase (yellow eel stage) would be remobilized during their reproductive migration leading to potential toxic events in gonads. The aim of this study was to investigate the effects of organic and inorganic contaminants on the gonad development of wild female silver eels. Female silver eels from two sites with differing contamination levels were artificially matured. Transcriptomic analyses by means of a 1000 candidate gene cDNA microarray were performed on gonads after 11 weeks of maturation to get insight into the mechanisms of toxicity of contaminants. The transcription levels of several genes, that were associated to the gonadosomatic index (GSI), were involved in mitotic cell division but also in gametogenesis. Genes associated to contaminants were mainly involved in the mechanisms of protection against oxidative stress, in DNA repair, in the purinergic signaling pathway and in steroidogenesis, suggesting an impairment of gonad development in eels from the polluted site. This was in agreement with the fact that eels from the reference site showed a higher gonad growth in comparison to contaminated fish. |
author2 |
Littoral, Environnement, Télédétection, Géomatique (LETG - Rennes) Littoral, Environnement, Télédétection, Géomatique UMR 6554 (LETG) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Université d'Angers (UA)-École Pratique des Hautes Études (EPHE) Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Brest (UBO)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut de Géographie et d'Aménagement Régional de l'Université de Nantes (IGARUN) Université de Nantes (UN)-Université de Nantes (UN)-Université de Caen Normandie (UNICAEN) Université de Nantes (UN)-Université de Nantes (UN) Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) Institut de Biologie Intégrative et des Systèmes Québec (IBIS) Environnements et Paléoenvironnements OCéaniques (EPOC) Observatoire aquitain des sciences de l'univers (OASU) Université Sciences et Technologies - Bordeaux 1 (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1 (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-École Pratique des Hautes Études (EPHE) Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS) Faculty of Engineering and the Environment (µVIS X-ray Imaging Centre) University of Southampton Ecosystèmes aquatiques et changements globaux (UR EABX) Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA) Institut National de la Recherche Scientifique Québec (INRS) Laboratoire de Physico -& Toxico Chimie des systèmes naturels (LPTC) Université Sciences et Technologies - Bordeaux 1 (UB)-Centre National de la Recherche Scientifique (CNRS) Institut des Sciences Moléculaires (ISM) Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1 (UB)-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Dufour, S. Bernatchez, L. Baudrimont, M. Baillon, L. Oses, J. Pierron, F. Colombier, S., Bureau Du Caron, A. Normandeau, E. Lambert, Patrick Couture, P. Labadie, P. Budzinski, H. |
author_facet |
Dufour, S. Bernatchez, L. Baudrimont, M. Baillon, L. Oses, J. Pierron, F. Colombier, S., Bureau Du Caron, A. Normandeau, E. Lambert, Patrick Couture, P. Labadie, P. Budzinski, H. |
author_sort |
Dufour, S. |
title |
Gonadal transcriptome analysis of wild contaminated female European eels during artificial gonad maturation |
title_short |
Gonadal transcriptome analysis of wild contaminated female European eels during artificial gonad maturation |
title_full |
Gonadal transcriptome analysis of wild contaminated female European eels during artificial gonad maturation |
title_fullStr |
Gonadal transcriptome analysis of wild contaminated female European eels during artificial gonad maturation |
title_full_unstemmed |
Gonadal transcriptome analysis of wild contaminated female European eels during artificial gonad maturation |
title_sort |
gonadal transcriptome analysis of wild contaminated female european eels during artificial gonad maturation |
publisher |
HAL CCSD |
publishDate |
2015 |
url |
https://hal.science/hal-02289130 https://doi.org/10.1016/j.chemosphere.2015.06.007 |
genre |
Anguilla anguilla European eel |
genre_facet |
Anguilla anguilla European eel |
op_source |
ISSN: 0045-6535 EISSN: 1879-1298 Chemosphere https://hal.science/hal-02289130 Chemosphere, 2015, 139, pp.303--309. ⟨10.1016/j.chemosphere.2015.06.007⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.chemosphere.2015.06.007 hal-02289130 https://hal.science/hal-02289130 doi:10.1016/j.chemosphere.2015.06.007 IRSTEA: PUB00045358 |
op_doi |
https://doi.org/10.1016/j.chemosphere.2015.06.007 |
container_title |
Chemosphere |
container_volume |
139 |
container_start_page |
303 |
op_container_end_page |
309 |
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1796941182226399232 |
spelling |
ftecolephe:oai:HAL:hal-02289130v1 2024-04-21T07:45:39+00:00 Gonadal transcriptome analysis of wild contaminated female European eels during artificial gonad maturation Dufour, S. Bernatchez, L. Baudrimont, M. Baillon, L. Oses, J. Pierron, F. Colombier, S., Bureau Du Caron, A. Normandeau, E. Lambert, Patrick Couture, P. Labadie, P. Budzinski, H. Littoral, Environnement, Télédétection, Géomatique (LETG - Rennes) Littoral, Environnement, Télédétection, Géomatique UMR 6554 (LETG) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Université d'Angers (UA)-École Pratique des Hautes Études (EPHE) Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Brest (UBO)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut de Géographie et d'Aménagement Régional de l'Université de Nantes (IGARUN) Université de Nantes (UN)-Université de Nantes (UN)-Université de Caen Normandie (UNICAEN) Université de Nantes (UN)-Université de Nantes (UN) Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) Institut de Biologie Intégrative et des Systèmes Québec (IBIS) Environnements et Paléoenvironnements OCéaniques (EPOC) Observatoire aquitain des sciences de l'univers (OASU) Université Sciences et Technologies - Bordeaux 1 (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1 (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-École Pratique des Hautes Études (EPHE) Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS) Faculty of Engineering and the Environment (µVIS X-ray Imaging Centre) University of Southampton Ecosystèmes aquatiques et changements globaux (UR EABX) Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA) Institut National de la Recherche Scientifique Québec (INRS) Laboratoire de Physico -& Toxico Chimie des systèmes naturels (LPTC) Université Sciences et Technologies - Bordeaux 1 (UB)-Centre National de la Recherche Scientifique (CNRS) Institut des Sciences Moléculaires (ISM) Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1 (UB)-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS) 2015 https://hal.science/hal-02289130 https://doi.org/10.1016/j.chemosphere.2015.06.007 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.chemosphere.2015.06.007 hal-02289130 https://hal.science/hal-02289130 doi:10.1016/j.chemosphere.2015.06.007 IRSTEA: PUB00045358 ISSN: 0045-6535 EISSN: 1879-1298 Chemosphere https://hal.science/hal-02289130 Chemosphere, 2015, 139, pp.303--309. ⟨10.1016/j.chemosphere.2015.06.007⟩ [SDE]Environmental Sciences info:eu-repo/semantics/article Journal articles 2015 ftecolephe https://doi.org/10.1016/j.chemosphere.2015.06.007 2024-04-11T00:04:58Z [Departement_IRSTEA]Eaux [TR1_IRSTEA]QUASARE International audience Since the early 1980s, the population of European eels (Anguilla anguilla) has dramatically declined. Nowadays, the European eel is listed on the red list of threatened species (IUCN Red List) and is considered as critically endangered of extinction. Pollution is one of the putative causes for the collapse of this species. Among their possible effects, contaminants gradually accumulated in eels during their somatic growth phase (yellow eel stage) would be remobilized during their reproductive migration leading to potential toxic events in gonads. The aim of this study was to investigate the effects of organic and inorganic contaminants on the gonad development of wild female silver eels. Female silver eels from two sites with differing contamination levels were artificially matured. Transcriptomic analyses by means of a 1000 candidate gene cDNA microarray were performed on gonads after 11 weeks of maturation to get insight into the mechanisms of toxicity of contaminants. The transcription levels of several genes, that were associated to the gonadosomatic index (GSI), were involved in mitotic cell division but also in gametogenesis. Genes associated to contaminants were mainly involved in the mechanisms of protection against oxidative stress, in DNA repair, in the purinergic signaling pathway and in steroidogenesis, suggesting an impairment of gonad development in eels from the polluted site. This was in agreement with the fact that eels from the reference site showed a higher gonad growth in comparison to contaminated fish. Article in Journal/Newspaper Anguilla anguilla European eel EPHE (Ecole pratique des hautes études, Paris): HAL Chemosphere 139 303 309 |