Transcriptome Profiling of the Pacific Oyster Crassostrea gigas Visceral Ganglia over a Reproduction Cycle Identifies Novel Regulatory Peptides

• Published in: Marine Drugs
• Main Authors: Réalis-Doyelle, Emilie, Schwartz, Julie, Cabau, Cédric, Le Franc, Lorane, Bernay, Benoit, Rivière, Guillaume, Klopp, Christophe, C., Favrel, Pascal
• Other Authors: Biologie des Organismes et Ecosystèmes Aquatiques (BOREA), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA), Système d'Information des GENomes des Animaux d'Elevage (SIGENAE), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-École nationale supérieure agronomique de Toulouse (ENSAT), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Interactions Cellules Organismes Environnement (ICORE), Normandie Université (NU)-Normandie Université (NU)-CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Tumorothèque de Caen Basse-Normandie (TCBN), Unité de Mathématiques et Informatique Appliquées de Toulouse (MIAT INRAE), Council of the Normandy Region (RIN ECUME: 18E01643-18P02383), ANR-14-CE02-0020,NEMO,Neuropeptides d'organismes marins(2014)
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2021
• Subjects: transcriptome; neuropeptides; reproduction; nervous system; [SDV.BDLR.RS]Life Sciences [q-bio]/Reproductive Biology/Sexual reproduction; [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology; [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry; Molecular Biology/Genomics [q-bio.GN]; Crassostrea gigas; Pacific oyster
• Online Access: https://hal.science/hal-03330324; https://hal.science/hal-03330324/document; https://hal.science/hal-03330324/file/marinedrugs-published.pdf; https://doi.org/10.3390/md19080452

International audience The neuropeptides involved in the regulation of reproduction in the Pacific oyster (Crassostrea gigas) are quite diverse. To investigate this diversity, a transcriptomic survey of the visceralganglia (VG) was carried out over an annual reproductive cycle. RNA-seq data from 26 samplescorresponding to VG at different stages of reproduction were de novo assembled to generate a specificreference transcriptome of the oyster nervous system and used to identify differentially expressedtranscripts. Transcriptome mining led to the identification of novel neuropeptide precursors (NPPs)related to the bilaterian Eclosion Hormone (EH), crustacean female sex hormone/Interleukin 17,Nesfatin, neuroparsin/IGFBP, prokineticins, and urotensin I; to the protostome GNQQN, pleurin, prohormones 3 and 4, prothoracotropic hormones (PTTH), and QSamide/PXXXamide; to the lophotrochozoan CCWamide, CLCCY, HFAamide, and LXRX; and to the mollusk-specific NPPs CCCGS,clionin, FYFY, GNamide, GRWRN, GSWN, GWE, IWMPxxGYxx, LXRYamide, RTLFamide, SLRFamide, and WGAGamide. Among the complete repertoire of NPPs, no sex-biased expression wasobserved. However, 25 NPPs displayed reproduction stage-specific expression, supporting theirinvolvement in the control of gametogenesis or associated metabolisms