Transcriptome Profiling of the Pacific Oyster Crassostrea gigas Visceral Ganglia over a Reproduction Cycle Identifies Novel Regulatory Peptides
International audience The neuropeptides involved in the regulation of reproduction in the Pacific oyster (Crassostrea gigas) are quite diverse. To investigate this diversity, a transcriptomic survey of the visceralganglia (VG) was carried out over an annual reproductive cycle. RNA-seq data from 26...
Published in: | Marine Drugs |
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Main Authors: | , , , , , , , |
Other Authors: | , , , , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2021
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Subjects: | |
Online Access: | https://hal.science/hal-03330324 https://hal.science/hal-03330324/document https://hal.science/hal-03330324/file/marinedrugs-published.pdf https://doi.org/10.3390/md19080452 |
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openpolar |
institution |
Open Polar |
collection |
Portail HAL ENVT (Ecole Nationale Vétérinaire de Toulouse) |
op_collection_id |
ftecolenvt |
language |
English |
topic |
transcriptome neuropeptides reproduction nervous system [SDV.BDLR.RS]Life Sciences [q-bio]/Reproductive Biology/Sexual reproduction [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] |
spellingShingle |
transcriptome neuropeptides reproduction nervous system [SDV.BDLR.RS]Life Sciences [q-bio]/Reproductive Biology/Sexual reproduction [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] Réalis-Doyelle, Emilie Schwartz, Julie Cabau, Cédric Le Franc, Lorane Bernay, Benoit Rivière, Guillaume Klopp, Christophe, C. Favrel, Pascal Transcriptome Profiling of the Pacific Oyster Crassostrea gigas Visceral Ganglia over a Reproduction Cycle Identifies Novel Regulatory Peptides |
topic_facet |
transcriptome neuropeptides reproduction nervous system [SDV.BDLR.RS]Life Sciences [q-bio]/Reproductive Biology/Sexual reproduction [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] |
description |
International audience The neuropeptides involved in the regulation of reproduction in the Pacific oyster (Crassostrea gigas) are quite diverse. To investigate this diversity, a transcriptomic survey of the visceralganglia (VG) was carried out over an annual reproductive cycle. RNA-seq data from 26 samplescorresponding to VG at different stages of reproduction were de novo assembled to generate a specificreference transcriptome of the oyster nervous system and used to identify differentially expressedtranscripts. Transcriptome mining led to the identification of novel neuropeptide precursors (NPPs)related to the bilaterian Eclosion Hormone (EH), crustacean female sex hormone/Interleukin 17,Nesfatin, neuroparsin/IGFBP, prokineticins, and urotensin I; to the protostome GNQQN, pleurin, prohormones 3 and 4, prothoracotropic hormones (PTTH), and QSamide/PXXXamide; to the lophotrochozoan CCWamide, CLCCY, HFAamide, and LXRX; and to the mollusk-specific NPPs CCCGS,clionin, FYFY, GNamide, GRWRN, GSWN, GWE, IWMPxxGYxx, LXRYamide, RTLFamide, SLRFamide, and WGAGamide. Among the complete repertoire of NPPs, no sex-biased expression wasobserved. However, 25 NPPs displayed reproduction stage-specific expression, supporting theirinvolvement in the control of gametogenesis or associated metabolisms |
author2 |
Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) Système d'Information des GENomes des Animaux d'Elevage (SIGENAE) Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) Génétique Physiologie et Systèmes d'Elevage (GenPhySE ) Ecole Nationale Vétérinaire de Toulouse (ENVT) Institut National Polytechnique (Toulouse) (Toulouse INP) Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP) Université de Toulouse (UT)-Université de Toulouse (UT)-École nationale supérieure agronomique de Toulouse (ENSAT) Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) Interactions Cellules Organismes Environnement (ICORE) Normandie Université (NU)-Normandie Université (NU)-CHU Caen Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Tumorothèque de Caen Basse-Normandie (TCBN) Unité de Mathématiques et Informatique Appliquées de Toulouse (MIAT INRAE) Council of the Normandy Region (RIN ECUME: 18E01643-18P02383) ANR-14-CE02-0020,NEMO,Neuropeptides d'organismes marins(2014) |
format |
Article in Journal/Newspaper |
author |
Réalis-Doyelle, Emilie Schwartz, Julie Cabau, Cédric Le Franc, Lorane Bernay, Benoit Rivière, Guillaume Klopp, Christophe, C. Favrel, Pascal |
author_facet |
Réalis-Doyelle, Emilie Schwartz, Julie Cabau, Cédric Le Franc, Lorane Bernay, Benoit Rivière, Guillaume Klopp, Christophe, C. Favrel, Pascal |
author_sort |
Réalis-Doyelle, Emilie |
title |
Transcriptome Profiling of the Pacific Oyster Crassostrea gigas Visceral Ganglia over a Reproduction Cycle Identifies Novel Regulatory Peptides |
title_short |
Transcriptome Profiling of the Pacific Oyster Crassostrea gigas Visceral Ganglia over a Reproduction Cycle Identifies Novel Regulatory Peptides |
title_full |
Transcriptome Profiling of the Pacific Oyster Crassostrea gigas Visceral Ganglia over a Reproduction Cycle Identifies Novel Regulatory Peptides |
title_fullStr |
Transcriptome Profiling of the Pacific Oyster Crassostrea gigas Visceral Ganglia over a Reproduction Cycle Identifies Novel Regulatory Peptides |
title_full_unstemmed |
Transcriptome Profiling of the Pacific Oyster Crassostrea gigas Visceral Ganglia over a Reproduction Cycle Identifies Novel Regulatory Peptides |
title_sort |
transcriptome profiling of the pacific oyster crassostrea gigas visceral ganglia over a reproduction cycle identifies novel regulatory peptides |
publisher |
HAL CCSD |
publishDate |
2021 |
url |
https://hal.science/hal-03330324 https://hal.science/hal-03330324/document https://hal.science/hal-03330324/file/marinedrugs-published.pdf https://doi.org/10.3390/md19080452 |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 1660-3397 Marine drugs https://hal.science/hal-03330324 Marine drugs, 2021, 19 (8), pp.452. ⟨10.3390/md19080452⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.3390/md19080452 info:eu-repo/semantics/altIdentifier/pmid/34436291 hal-03330324 https://hal.science/hal-03330324 https://hal.science/hal-03330324/document https://hal.science/hal-03330324/file/marinedrugs-published.pdf doi:10.3390/md19080452 PUBMED: 34436291 WOS: 000690573800001 |
op_rights |
http://creativecommons.org/licenses/by/ info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.3390/md19080452 |
container_title |
Marine Drugs |
container_volume |
19 |
container_issue |
8 |
container_start_page |
452 |
_version_ |
1796941073458659328 |
spelling |
ftecolenvt:oai:HAL:hal-03330324v1 2024-04-21T08:00:36+00:00 Transcriptome Profiling of the Pacific Oyster Crassostrea gigas Visceral Ganglia over a Reproduction Cycle Identifies Novel Regulatory Peptides Réalis-Doyelle, Emilie Schwartz, Julie Cabau, Cédric Le Franc, Lorane Bernay, Benoit Rivière, Guillaume Klopp, Christophe, C. Favrel, Pascal Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) Système d'Information des GENomes des Animaux d'Elevage (SIGENAE) Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) Génétique Physiologie et Systèmes d'Elevage (GenPhySE ) Ecole Nationale Vétérinaire de Toulouse (ENVT) Institut National Polytechnique (Toulouse) (Toulouse INP) Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP) Université de Toulouse (UT)-Université de Toulouse (UT)-École nationale supérieure agronomique de Toulouse (ENSAT) Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) Interactions Cellules Organismes Environnement (ICORE) Normandie Université (NU)-Normandie Université (NU)-CHU Caen Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Tumorothèque de Caen Basse-Normandie (TCBN) Unité de Mathématiques et Informatique Appliquées de Toulouse (MIAT INRAE) Council of the Normandy Region (RIN ECUME: 18E01643-18P02383) ANR-14-CE02-0020,NEMO,Neuropeptides d'organismes marins(2014) 2021 https://hal.science/hal-03330324 https://hal.science/hal-03330324/document https://hal.science/hal-03330324/file/marinedrugs-published.pdf https://doi.org/10.3390/md19080452 en eng HAL CCSD MDPI info:eu-repo/semantics/altIdentifier/doi/10.3390/md19080452 info:eu-repo/semantics/altIdentifier/pmid/34436291 hal-03330324 https://hal.science/hal-03330324 https://hal.science/hal-03330324/document https://hal.science/hal-03330324/file/marinedrugs-published.pdf doi:10.3390/md19080452 PUBMED: 34436291 WOS: 000690573800001 http://creativecommons.org/licenses/by/ info:eu-repo/semantics/OpenAccess ISSN: 1660-3397 Marine drugs https://hal.science/hal-03330324 Marine drugs, 2021, 19 (8), pp.452. ⟨10.3390/md19080452⟩ transcriptome neuropeptides reproduction nervous system [SDV.BDLR.RS]Life Sciences [q-bio]/Reproductive Biology/Sexual reproduction [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] info:eu-repo/semantics/article Journal articles 2021 ftecolenvt https://doi.org/10.3390/md19080452 2024-03-26T18:05:17Z International audience The neuropeptides involved in the regulation of reproduction in the Pacific oyster (Crassostrea gigas) are quite diverse. To investigate this diversity, a transcriptomic survey of the visceralganglia (VG) was carried out over an annual reproductive cycle. RNA-seq data from 26 samplescorresponding to VG at different stages of reproduction were de novo assembled to generate a specificreference transcriptome of the oyster nervous system and used to identify differentially expressedtranscripts. Transcriptome mining led to the identification of novel neuropeptide precursors (NPPs)related to the bilaterian Eclosion Hormone (EH), crustacean female sex hormone/Interleukin 17,Nesfatin, neuroparsin/IGFBP, prokineticins, and urotensin I; to the protostome GNQQN, pleurin, prohormones 3 and 4, prothoracotropic hormones (PTTH), and QSamide/PXXXamide; to the lophotrochozoan CCWamide, CLCCY, HFAamide, and LXRX; and to the mollusk-specific NPPs CCCGS,clionin, FYFY, GNamide, GRWRN, GSWN, GWE, IWMPxxGYxx, LXRYamide, RTLFamide, SLRFamide, and WGAGamide. Among the complete repertoire of NPPs, no sex-biased expression wasobserved. However, 25 NPPs displayed reproduction stage-specific expression, supporting theirinvolvement in the control of gametogenesis or associated metabolisms Article in Journal/Newspaper Crassostrea gigas Pacific oyster Portail HAL ENVT (Ecole Nationale Vétérinaire de Toulouse) Marine Drugs 19 8 452 |