Daily transcriptomes of the copepod Calanus finmarchicus during the summer solstice at high Arctic latitudes

• Published in: Scientific Data
• Main Authors: Payton, Laura, Noirot, Celine, Hoede, Claire, Hüppe, Lukas, Last, Kim, Wilcockson, David, C, Ershova, Elizaveta A., Valiere, Sophie, Meyer, Bettina
• Other Authors: Institute for Chemistry and Biology of the Marine Environment (ICBM), University of Oldenburg, Section Polar Biological Oceanography, Alfred Wegener institute Helmholtz centre for Polar and Marine Research, Unité de Mathématiques et Informatique Appliquées de Toulouse (MIAT INRAE), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Helmholtz Institute for Functional Marine Biodiversity at the University of Oldenburg (HIFMB), Carl Von Ossietzky Universität Oldenburg = Carl von Ossietzky University of Oldenburg (OFFIS), Scottish Association for Marine Science (SAMS), Aberystwyth University, The Arctic University of Norway Tromsø, Norway (UiT), P.P. Shirshov Institute of Oceanology (SIO), Russian Academy of Sciences Moscow (RAS), Génome et Transcriptome - Plateforme Génomique ( GeT-PlaGe), Plateforme Génome & Transcriptome (GET), Génopole Toulouse Midi-Pyrénées Auzeville (GENOTOUL), Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Génopole Toulouse Midi-Pyrénées Auzeville (GENOTOUL), Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), This work was supported by the CHASE project, part of the Changing Arctic Ocean programme, jointly funded by the UKRI Natural Environment Research Council (NERC, project number: NE/R012733/1) and the German Federal Ministry of Education and Research (BMBF, project number: 03F0803A). We thank the NERC PRIZE cruise leader Professor Finlo Cottier (Scottish Association for Marine Science, UK) as well as the Captain and crew of the RRS James Clark Ross for their support during the cruise JR17006. Cruise time was supported by the CAO Arctic PRIZE project (NERC: NE/P006302/1). EE was supported by Arctic SIZE, a project co-funded by UiT The Arctic University of Norway and the Tromso Research Foundation (project number 01vm/h15), and within framework of the state assignment of IO RAS (theme No. 0149- 2019- 0008). We would like to thank Vittoria Roncalli (Stazione Zoologica Anton Dohrn, Italy) for useful discussions. Open Access funding enabled and organized by Projekt DEAL.
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2020
• Subjects: CIRCADIAN CLOCK; SHIFTS; OCEAN; PCR; [SDE]Environmental Sciences; Arctic; Calanus finmarchicus; Climate change; Sea ice; Copepods; midnight sun
• Online Access: https://hal.inrae.fr/hal-03183048; https://hal.inrae.fr/hal-03183048/document; https://hal.inrae.fr/hal-03183048/file/2020_Payton_Scientific%20data.pdf; https://doi.org/10.1038/s41597-020-00751-4

International audience The zooplankter Calanus finmarchicus is a member of the so-called "Calanus Complex", a group of copepods that constitutes a key element of the Arctic polar marine ecosystem, providing a crucial link between primary production and higher trophic levels. Climate change induces the shift of C. finmarchicus to higher latitudes with currently unknown impacts on its endogenous timing. Here we generated a daily transcriptome of C. finmarchicus at two high Arctic stations, during the more extreme time of Midnight Sun, the summer solstice. While the southern station (74.5 °N) was sea ice-free, the northern one (82.5 °N) was sea ice-covered. The mRNAs of the 42 samples have been sequenced with an average of 126 ± 5 million reads (mean ± SE) per sample, and aligned to the reference transcriptome. We detail the quality assessment of the datasets and the complete annotation procedure, providing the possibility to investigate daily gene expression of this ecologically important species at high Arctic latitudes, and to compare gene expression according to latitude and sea ice-coverage.