The Total and Active Bacterial Community of the Chlorolichen Cetraria islandica and Its Response to Long-Term Warming in Sub-Arctic Tundra
International audience Lichens are traditionally defined as a symbiosis between a fungus and a green alga and or a cyanobacterium. This idea has been challenged by the discovery of bacterial communities inhabiting the lichen thalli. These bacteria are thought to contribute to the survival of lichens...
Published in: | Frontiers in Microbiology |
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Format: | Article in Journal/Newspaper |
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Online Access: | https://hal.science/hal-03830673 https://doi.org/10.3389/fmicb.2020.540404 |
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ftecolecentrlyon:oai:HAL:hal-03830673v1 2023-08-15T12:40:02+02:00 The Total and Active Bacterial Community of the Chlorolichen Cetraria islandica and Its Response to Long-Term Warming in Sub-Arctic Tundra Klarenberg, Ingeborg Keuschnig, Christoph Warshan, Denis Jónsdóttir, Ingibjörg Svala Vilhelmsson, Oddur University of Akureyri University of Iceland Reykjavik Ampère, Département Bioingénierie (BioIng) Ampère (AMPERE) École Centrale de Lyon (ECL) Université de Lyon-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL) Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon) Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-École Centrale de Lyon (ECL) Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) University of Reading (UOR) 2020-12-18 https://hal.science/hal-03830673 https://doi.org/10.3389/fmicb.2020.540404 en eng HAL CCSD Frontiers Media info:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2020.540404 hal-03830673 https://hal.science/hal-03830673 doi:10.3389/fmicb.2020.540404 ISSN: 1664-302X EISSN: 1664-302X Frontiers in Microbiology https://hal.science/hal-03830673 Frontiers in Microbiology, 2020, 11, ⟨10.3389/fmicb.2020.540404⟩ [SDE]Environmental Sciences info:eu-repo/semantics/article Journal articles 2020 ftecolecentrlyon https://doi.org/10.3389/fmicb.2020.540404 2023-07-25T20:22:53Z International audience Lichens are traditionally defined as a symbiosis between a fungus and a green alga and or a cyanobacterium. This idea has been challenged by the discovery of bacterial communities inhabiting the lichen thalli. These bacteria are thought to contribute to the survival of lichens under extreme and changing environmental conditions. How these changing environmental conditions affect the lichen-associated bacterial community composition remains unclear. We describe the total (rDNA-based) and potentially metabolically active (rRNA-based) bacterial community of the lichen Cetaria islandica and its response to long-term warming using a 20-year warming experiment in an Icelandic sub-Arctic tundra. 16S rRNA and rDNA amplicon sequencing showed that the orders Acetobacterales (of the class Alphaproteobacteria) and Acidobacteriales (of the phylum Acidobacteria) dominated the bacterial community. Numerous amplicon sequence variants (ASVs) could only be detected in the potentially active community but not in the total community. Long-term warming led to increases in relative abundance of bacterial taxa on class, order and ASV level. Warming altered the relative abundance of ASVs of the most common bacterial genera, such as Granulicella and Endobacter . The potentially metabolically active bacterial community was also more responsive to warming than the total community. Our results suggest that the bacterial community of the lichen C. islandica is dominated by acidophilic taxa and harbors disproportionally active rare taxa. We also show for the first time that climate warming can lead to shifts in lichen-associated bacterial community composition. Article in Journal/Newspaper Arctic Tundra Portail HAL - Ecole Centrale de Lyon Arctic Frontiers in Microbiology 11 |
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Portail HAL - Ecole Centrale de Lyon |
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ftecolecentrlyon |
language |
English |
topic |
[SDE]Environmental Sciences |
spellingShingle |
[SDE]Environmental Sciences Klarenberg, Ingeborg Keuschnig, Christoph Warshan, Denis Jónsdóttir, Ingibjörg Svala Vilhelmsson, Oddur The Total and Active Bacterial Community of the Chlorolichen Cetraria islandica and Its Response to Long-Term Warming in Sub-Arctic Tundra |
topic_facet |
[SDE]Environmental Sciences |
description |
International audience Lichens are traditionally defined as a symbiosis between a fungus and a green alga and or a cyanobacterium. This idea has been challenged by the discovery of bacterial communities inhabiting the lichen thalli. These bacteria are thought to contribute to the survival of lichens under extreme and changing environmental conditions. How these changing environmental conditions affect the lichen-associated bacterial community composition remains unclear. We describe the total (rDNA-based) and potentially metabolically active (rRNA-based) bacterial community of the lichen Cetaria islandica and its response to long-term warming using a 20-year warming experiment in an Icelandic sub-Arctic tundra. 16S rRNA and rDNA amplicon sequencing showed that the orders Acetobacterales (of the class Alphaproteobacteria) and Acidobacteriales (of the phylum Acidobacteria) dominated the bacterial community. Numerous amplicon sequence variants (ASVs) could only be detected in the potentially active community but not in the total community. Long-term warming led to increases in relative abundance of bacterial taxa on class, order and ASV level. Warming altered the relative abundance of ASVs of the most common bacterial genera, such as Granulicella and Endobacter . The potentially metabolically active bacterial community was also more responsive to warming than the total community. Our results suggest that the bacterial community of the lichen C. islandica is dominated by acidophilic taxa and harbors disproportionally active rare taxa. We also show for the first time that climate warming can lead to shifts in lichen-associated bacterial community composition. |
author2 |
University of Akureyri University of Iceland Reykjavik Ampère, Département Bioingénierie (BioIng) Ampère (AMPERE) École Centrale de Lyon (ECL) Université de Lyon-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL) Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon) Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-École Centrale de Lyon (ECL) Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) University of Reading (UOR) |
format |
Article in Journal/Newspaper |
author |
Klarenberg, Ingeborg Keuschnig, Christoph Warshan, Denis Jónsdóttir, Ingibjörg Svala Vilhelmsson, Oddur |
author_facet |
Klarenberg, Ingeborg Keuschnig, Christoph Warshan, Denis Jónsdóttir, Ingibjörg Svala Vilhelmsson, Oddur |
author_sort |
Klarenberg, Ingeborg |
title |
The Total and Active Bacterial Community of the Chlorolichen Cetraria islandica and Its Response to Long-Term Warming in Sub-Arctic Tundra |
title_short |
The Total and Active Bacterial Community of the Chlorolichen Cetraria islandica and Its Response to Long-Term Warming in Sub-Arctic Tundra |
title_full |
The Total and Active Bacterial Community of the Chlorolichen Cetraria islandica and Its Response to Long-Term Warming in Sub-Arctic Tundra |
title_fullStr |
The Total and Active Bacterial Community of the Chlorolichen Cetraria islandica and Its Response to Long-Term Warming in Sub-Arctic Tundra |
title_full_unstemmed |
The Total and Active Bacterial Community of the Chlorolichen Cetraria islandica and Its Response to Long-Term Warming in Sub-Arctic Tundra |
title_sort |
total and active bacterial community of the chlorolichen cetraria islandica and its response to long-term warming in sub-arctic tundra |
publisher |
HAL CCSD |
publishDate |
2020 |
url |
https://hal.science/hal-03830673 https://doi.org/10.3389/fmicb.2020.540404 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Tundra |
genre_facet |
Arctic Tundra |
op_source |
ISSN: 1664-302X EISSN: 1664-302X Frontiers in Microbiology https://hal.science/hal-03830673 Frontiers in Microbiology, 2020, 11, ⟨10.3389/fmicb.2020.540404⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2020.540404 hal-03830673 https://hal.science/hal-03830673 doi:10.3389/fmicb.2020.540404 |
op_doi |
https://doi.org/10.3389/fmicb.2020.540404 |
container_title |
Frontiers in Microbiology |
container_volume |
11 |
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1774292722436276224 |