Establishment of the first humpback whale fibroblast cell lines and their application in chemical risk assessment
This paper reports the first successful derivation and characterization of humpback whale fibroblast cell lines. Primary fibroblasts were isolated from the dermal connective tissue of skin biopsies, cultured at 37 °C and 5% CO 2 in the standard mammalian medium DMEM/F12 supplemented with 10% fetal b...
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Online Access: | https://doi.org/10.1016/j.aquatox.2015.08.005 |
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fteawag:oai:dora:eawag_8262 2023-05-15T13:42:14+02:00 Establishment of the first humpback whale fibroblast cell lines and their application in chemical risk assessment Burkard, Michael Whitworth, Deanne Schirmer, Kristin Nash, Susan Bengtson 2015 https://doi.org/10.1016/j.aquatox.2015.08.005 eng eng Elsevier Aquatic Toxicology--Aquat. Toxicol.--journals:219--0166-445X eawag:8262 journal id: journals:219 issn: 0166-445X ut: 000362859400023 local: 17735 doi:10.1016/j.aquatox.2015.08.005 scopus: 2-s2.0-84941241759 Megaptera novaeangliae p,p′,-DDE Antarctica persistent organic pollutants (POPs) karyotype cell line characterization Text Journal Article 2015 fteawag https://doi.org/10.1016/j.aquatox.2015.08.005 2023-04-09T04:46:37Z This paper reports the first successful derivation and characterization of humpback whale fibroblast cell lines. Primary fibroblasts were isolated from the dermal connective tissue of skin biopsies, cultured at 37 °C and 5% CO 2 in the standard mammalian medium DMEM/F12 supplemented with 10% fetal bovine serum (FBS). Of nine initial biopsies, two cell lines were established from two different animals and designated HuWa1 and HuWa2. The cells have a stable karyotype with 2 n = 44, which has commonly been observed in other baleen whale species. Cells were verified as being fibroblasts based on their spindle-shaped morphology, adherence to plastic and positive immunoreaction to vimentin. Population doubling time was determined to be ∼41 h and cells were successfully cryopreserved and thawed. To date, HuWa1 cells have been propagated 30 times. Cells proliferate at the tested temperatures, 30, 33.5 and 37 °C, but show the highest rate of proliferation at 37 °C. Short-term exposure to para , para′ -dichlorodiphenyldichloroethylene ( p,p′- DDE), a priority compound accumulating in southern hemisphere humpback whales, resulted in a concentration-dependent loss of cell viability. The effective concentration which caused a 50% reduction in HuWa1 cell viability (EC 50 value) was approximately six times greater than the EC 50 value for the same chemical measured with human dermal fibroblasts. HuWa1 exposed to a natural, p,p′ -DDE-containing, chemical mixture extracted from whale blubber showed distinctively higher sensitivity than to p,p′- DDE alone. Thus, we provide the first cytotoxicological data for humpback whales and with establishment of the HuWa cell lines, a unique in vitro model for the study of the whales' sensitivity and cellular response to chemicals and other environmental stressors. Article in Journal/Newspaper Antarc* Antarctica baleen whale Humpback Whale Megaptera novaeangliae DORA Eawag Aquatic Toxicology 167 240 247 |
institution |
Open Polar |
collection |
DORA Eawag |
op_collection_id |
fteawag |
language |
English |
topic |
Megaptera novaeangliae p,p′,-DDE Antarctica persistent organic pollutants (POPs) karyotype cell line characterization |
spellingShingle |
Megaptera novaeangliae p,p′,-DDE Antarctica persistent organic pollutants (POPs) karyotype cell line characterization Burkard, Michael Whitworth, Deanne Schirmer, Kristin Nash, Susan Bengtson Establishment of the first humpback whale fibroblast cell lines and their application in chemical risk assessment |
topic_facet |
Megaptera novaeangliae p,p′,-DDE Antarctica persistent organic pollutants (POPs) karyotype cell line characterization |
description |
This paper reports the first successful derivation and characterization of humpback whale fibroblast cell lines. Primary fibroblasts were isolated from the dermal connective tissue of skin biopsies, cultured at 37 °C and 5% CO 2 in the standard mammalian medium DMEM/F12 supplemented with 10% fetal bovine serum (FBS). Of nine initial biopsies, two cell lines were established from two different animals and designated HuWa1 and HuWa2. The cells have a stable karyotype with 2 n = 44, which has commonly been observed in other baleen whale species. Cells were verified as being fibroblasts based on their spindle-shaped morphology, adherence to plastic and positive immunoreaction to vimentin. Population doubling time was determined to be ∼41 h and cells were successfully cryopreserved and thawed. To date, HuWa1 cells have been propagated 30 times. Cells proliferate at the tested temperatures, 30, 33.5 and 37 °C, but show the highest rate of proliferation at 37 °C. Short-term exposure to para , para′ -dichlorodiphenyldichloroethylene ( p,p′- DDE), a priority compound accumulating in southern hemisphere humpback whales, resulted in a concentration-dependent loss of cell viability. The effective concentration which caused a 50% reduction in HuWa1 cell viability (EC 50 value) was approximately six times greater than the EC 50 value for the same chemical measured with human dermal fibroblasts. HuWa1 exposed to a natural, p,p′ -DDE-containing, chemical mixture extracted from whale blubber showed distinctively higher sensitivity than to p,p′- DDE alone. Thus, we provide the first cytotoxicological data for humpback whales and with establishment of the HuWa cell lines, a unique in vitro model for the study of the whales' sensitivity and cellular response to chemicals and other environmental stressors. |
format |
Article in Journal/Newspaper |
author |
Burkard, Michael Whitworth, Deanne Schirmer, Kristin Nash, Susan Bengtson |
author_facet |
Burkard, Michael Whitworth, Deanne Schirmer, Kristin Nash, Susan Bengtson |
author_sort |
Burkard, Michael |
title |
Establishment of the first humpback whale fibroblast cell lines and their application in chemical risk assessment |
title_short |
Establishment of the first humpback whale fibroblast cell lines and their application in chemical risk assessment |
title_full |
Establishment of the first humpback whale fibroblast cell lines and their application in chemical risk assessment |
title_fullStr |
Establishment of the first humpback whale fibroblast cell lines and their application in chemical risk assessment |
title_full_unstemmed |
Establishment of the first humpback whale fibroblast cell lines and their application in chemical risk assessment |
title_sort |
establishment of the first humpback whale fibroblast cell lines and their application in chemical risk assessment |
publisher |
Elsevier |
publishDate |
2015 |
url |
https://doi.org/10.1016/j.aquatox.2015.08.005 |
genre |
Antarc* Antarctica baleen whale Humpback Whale Megaptera novaeangliae |
genre_facet |
Antarc* Antarctica baleen whale Humpback Whale Megaptera novaeangliae |
op_relation |
Aquatic Toxicology--Aquat. Toxicol.--journals:219--0166-445X eawag:8262 journal id: journals:219 issn: 0166-445X ut: 000362859400023 local: 17735 doi:10.1016/j.aquatox.2015.08.005 scopus: 2-s2.0-84941241759 |
op_doi |
https://doi.org/10.1016/j.aquatox.2015.08.005 |
container_title |
Aquatic Toxicology |
container_volume |
167 |
container_start_page |
240 |
op_container_end_page |
247 |
_version_ |
1766165767799177216 |