T-RFLP fingerprinting analysis of bacterial communities in debris cones, Northern Victoria Land, Antarctica
The debris cones known as Amorphous Glacier and Boulder Clay are located in an ice-free region in Northern Victoria Land, Antarctica, and differ in their isotopic composition, mechanisms of ice distribution, geological formation and age. However, to date it is not known if bacterial community profil...
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Online Access: | https://doi.org/10.1002/ppp.1749 |
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fteawag:oai:dora:eawag_7451 2024-09-15T17:44:02+00:00 T-RFLP fingerprinting analysis of bacterial communities in debris cones, Northern Victoria Land, Antarctica Abramovich, Reut Sorek Pomati, Francesco Jungblut, Anne D. Guglielmin, Mauro Neilan, Brett A. 2012 https://doi.org/10.1002/ppp.1749 eng eng Wiley Permafrost and Periglacial Processes--Permafr. Periglac. Process.--journals:2311--1045-6740--1099-1530 eawag:7451 journal id: journals:2311 issn: 1045-6740 e-issn: 1099-1530 ut: 000308396400009 local: 16410 doi:10.1002/ppp.1749 scopus: 2-s2.0-84865859146 T-RFLP fingerprinting bacteria debris cone ice permafrost Antarctica Text Journal Article 2012 fteawag https://doi.org/10.1002/ppp.1749 2024-08-05T03:04:28Z The debris cones known as Amorphous Glacier and Boulder Clay are located in an ice-free region in Northern Victoria Land, Antarctica, and differ in their isotopic composition, mechanisms of ice distribution, geological formation and age. However, to date it is not known if bacterial community profiles within ice and permafrost can be established for these environments, and then whether glaciological differences between the two areas would be reflected in the bacterial community composition. In order to gather first evidence for the bacterial communities in these glacial zones, we carried out terminal-restriction fragment length polymorphism (T-RFLP) analysis on the 16S rRNA gene using a universal bacterial amplification protocol on two permafrost cores. The DNA yields from ice-core samples ranged from 0.29 ng μL -1 in Amorphous Glacier to 88 ng μL -1 in Boulder Clay. Bray-Curtis cluster analysis suggested Boulder Clay bacterial profiles were similar to each other, but cluster separately from the Amorphous Glacier bacterial profile. Article in Journal/Newspaper Antarc* Antarctica Ice ice core permafrost Permafrost and Periglacial Processes Victoria Land DORA Eawag Permafrost and Periglacial Processes 23 3 244 248 |
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Open Polar |
collection |
DORA Eawag |
op_collection_id |
fteawag |
language |
English |
topic |
T-RFLP fingerprinting bacteria debris cone ice permafrost Antarctica |
spellingShingle |
T-RFLP fingerprinting bacteria debris cone ice permafrost Antarctica Abramovich, Reut Sorek Pomati, Francesco Jungblut, Anne D. Guglielmin, Mauro Neilan, Brett A. T-RFLP fingerprinting analysis of bacterial communities in debris cones, Northern Victoria Land, Antarctica |
topic_facet |
T-RFLP fingerprinting bacteria debris cone ice permafrost Antarctica |
description |
The debris cones known as Amorphous Glacier and Boulder Clay are located in an ice-free region in Northern Victoria Land, Antarctica, and differ in their isotopic composition, mechanisms of ice distribution, geological formation and age. However, to date it is not known if bacterial community profiles within ice and permafrost can be established for these environments, and then whether glaciological differences between the two areas would be reflected in the bacterial community composition. In order to gather first evidence for the bacterial communities in these glacial zones, we carried out terminal-restriction fragment length polymorphism (T-RFLP) analysis on the 16S rRNA gene using a universal bacterial amplification protocol on two permafrost cores. The DNA yields from ice-core samples ranged from 0.29 ng μL -1 in Amorphous Glacier to 88 ng μL -1 in Boulder Clay. Bray-Curtis cluster analysis suggested Boulder Clay bacterial profiles were similar to each other, but cluster separately from the Amorphous Glacier bacterial profile. |
format |
Article in Journal/Newspaper |
author |
Abramovich, Reut Sorek Pomati, Francesco Jungblut, Anne D. Guglielmin, Mauro Neilan, Brett A. |
author_facet |
Abramovich, Reut Sorek Pomati, Francesco Jungblut, Anne D. Guglielmin, Mauro Neilan, Brett A. |
author_sort |
Abramovich, Reut Sorek |
title |
T-RFLP fingerprinting analysis of bacterial communities in debris cones, Northern Victoria Land, Antarctica |
title_short |
T-RFLP fingerprinting analysis of bacterial communities in debris cones, Northern Victoria Land, Antarctica |
title_full |
T-RFLP fingerprinting analysis of bacterial communities in debris cones, Northern Victoria Land, Antarctica |
title_fullStr |
T-RFLP fingerprinting analysis of bacterial communities in debris cones, Northern Victoria Land, Antarctica |
title_full_unstemmed |
T-RFLP fingerprinting analysis of bacterial communities in debris cones, Northern Victoria Land, Antarctica |
title_sort |
t-rflp fingerprinting analysis of bacterial communities in debris cones, northern victoria land, antarctica |
publisher |
Wiley |
publishDate |
2012 |
url |
https://doi.org/10.1002/ppp.1749 |
genre |
Antarc* Antarctica Ice ice core permafrost Permafrost and Periglacial Processes Victoria Land |
genre_facet |
Antarc* Antarctica Ice ice core permafrost Permafrost and Periglacial Processes Victoria Land |
op_relation |
Permafrost and Periglacial Processes--Permafr. Periglac. Process.--journals:2311--1045-6740--1099-1530 eawag:7451 journal id: journals:2311 issn: 1045-6740 e-issn: 1099-1530 ut: 000308396400009 local: 16410 doi:10.1002/ppp.1749 scopus: 2-s2.0-84865859146 |
op_doi |
https://doi.org/10.1002/ppp.1749 |
container_title |
Permafrost and Periglacial Processes |
container_volume |
23 |
container_issue |
3 |
container_start_page |
244 |
op_container_end_page |
248 |
_version_ |
1810491328411205632 |