An estrogen-responsive plasma protein expression signature in Atlantic cod (Gadus morhua) revealed by SELDI-TOF MS
Compound-specific protein expression signatures( PESs) can be revealed by proteomic techniques. The SELDI-TOF MS approach is advantageous due to its simplicity and high-throughput capacity,however, there are concerns regarding the reproducibility of this method. The aim of this study was to define a...
Published in: | Ecotoxicology and Environmental Safety |
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ftdtupubl:oai:pure.atira.dk:publications/db8e164e-3e78-4f9c-ad4f-5237d0fef1af 2023-05-15T15:27:22+02:00 An estrogen-responsive plasma protein expression signature in Atlantic cod (Gadus morhua) revealed by SELDI-TOF MS Nielsen, Mari Mæland Meyer, Sonnich Larsen, Bodil Katrine Andersen, Odd Ketil Hjelle, Anne 2011 https://orbit.dtu.dk/en/publications/db8e164e-3e78-4f9c-ad4f-5237d0fef1af https://doi.org/10.1016/j.ecoenv.2011.07.036 eng eng info:eu-repo/semantics/closedAccess Nielsen , M M , Meyer , S , Larsen , B K , Andersen , O K & Hjelle , A 2011 , ' An estrogen-responsive plasma protein expression signature in Atlantic cod (Gadus morhua) revealed by SELDI-TOF MS ' , Ecotoxicology and Environmental Safety , vol. 74 , pp. 2175-2181 . https://doi.org/10.1016/j.ecoenv.2011.07.036 article 2011 ftdtupubl https://doi.org/10.1016/j.ecoenv.2011.07.036 2023-01-04T23:56:26Z Compound-specific protein expression signatures( PESs) can be revealed by proteomic techniques. The SELDI-TOF MS approach is advantageous due to its simplicity and high-throughput capacity,however, there are concerns regarding the reproducibility of this method. The aim of this study was to define an estrogen-responsive PES in plasma of Atlantic cod (Gadus morhua) using the SELDI-TOF MS technique. Protein expression analysis of male cod exposed to 17 b-estradiol (E2) showed that 27 plasma peaks were differentially expressed following exposure.There producibility of this result was evaluated by reanalyzing the samples six months later,and a significant change in expression was confirmed for 13 of the 27 peaks detected in the first analysis. The performance of the reproducible E2-responsive PES, constituting these 13 peaks, was then tested on samples from juvenile cod exposed to 4-nonylphenol, North Sea oil, or North Sea oil spiked with alkylphenols. Principal component analysis revealed that nonylphenol-exposed cod could be separated from unexposed cod based on the E2-responsive PES, indicating that the PES can be used to assess estrogenic exposure of both juvenile and adult specimens of cod. A targeted antibody-assisted SELDI-TOF MS approach was carried out in an attempt to identify the E2-responsive peaks. Results indicated that 2 peaks were fragments of the well-known biomarkers VTG and/or ZRP. In this study, the SELDI-TOF MS technology has shown its potential for defining compound-specific PESs in fish. Nevertheless, thorough validtion of reproducibility, specificity and sensitivity of a PESis required before it can be applied in environmental monitoring. Article in Journal/Newspaper atlantic cod Gadus morhua Technical University of Denmark: DTU Orbit Ecotoxicology and Environmental Safety 74 8 2175 2181 |
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Open Polar |
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Technical University of Denmark: DTU Orbit |
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ftdtupubl |
language |
English |
description |
Compound-specific protein expression signatures( PESs) can be revealed by proteomic techniques. The SELDI-TOF MS approach is advantageous due to its simplicity and high-throughput capacity,however, there are concerns regarding the reproducibility of this method. The aim of this study was to define an estrogen-responsive PES in plasma of Atlantic cod (Gadus morhua) using the SELDI-TOF MS technique. Protein expression analysis of male cod exposed to 17 b-estradiol (E2) showed that 27 plasma peaks were differentially expressed following exposure.There producibility of this result was evaluated by reanalyzing the samples six months later,and a significant change in expression was confirmed for 13 of the 27 peaks detected in the first analysis. The performance of the reproducible E2-responsive PES, constituting these 13 peaks, was then tested on samples from juvenile cod exposed to 4-nonylphenol, North Sea oil, or North Sea oil spiked with alkylphenols. Principal component analysis revealed that nonylphenol-exposed cod could be separated from unexposed cod based on the E2-responsive PES, indicating that the PES can be used to assess estrogenic exposure of both juvenile and adult specimens of cod. A targeted antibody-assisted SELDI-TOF MS approach was carried out in an attempt to identify the E2-responsive peaks. Results indicated that 2 peaks were fragments of the well-known biomarkers VTG and/or ZRP. In this study, the SELDI-TOF MS technology has shown its potential for defining compound-specific PESs in fish. Nevertheless, thorough validtion of reproducibility, specificity and sensitivity of a PESis required before it can be applied in environmental monitoring. |
format |
Article in Journal/Newspaper |
author |
Nielsen, Mari Mæland Meyer, Sonnich Larsen, Bodil Katrine Andersen, Odd Ketil Hjelle, Anne |
spellingShingle |
Nielsen, Mari Mæland Meyer, Sonnich Larsen, Bodil Katrine Andersen, Odd Ketil Hjelle, Anne An estrogen-responsive plasma protein expression signature in Atlantic cod (Gadus morhua) revealed by SELDI-TOF MS |
author_facet |
Nielsen, Mari Mæland Meyer, Sonnich Larsen, Bodil Katrine Andersen, Odd Ketil Hjelle, Anne |
author_sort |
Nielsen, Mari Mæland |
title |
An estrogen-responsive plasma protein expression signature in Atlantic cod (Gadus morhua) revealed by SELDI-TOF MS |
title_short |
An estrogen-responsive plasma protein expression signature in Atlantic cod (Gadus morhua) revealed by SELDI-TOF MS |
title_full |
An estrogen-responsive plasma protein expression signature in Atlantic cod (Gadus morhua) revealed by SELDI-TOF MS |
title_fullStr |
An estrogen-responsive plasma protein expression signature in Atlantic cod (Gadus morhua) revealed by SELDI-TOF MS |
title_full_unstemmed |
An estrogen-responsive plasma protein expression signature in Atlantic cod (Gadus morhua) revealed by SELDI-TOF MS |
title_sort |
estrogen-responsive plasma protein expression signature in atlantic cod (gadus morhua) revealed by seldi-tof ms |
publishDate |
2011 |
url |
https://orbit.dtu.dk/en/publications/db8e164e-3e78-4f9c-ad4f-5237d0fef1af https://doi.org/10.1016/j.ecoenv.2011.07.036 |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_source |
Nielsen , M M , Meyer , S , Larsen , B K , Andersen , O K & Hjelle , A 2011 , ' An estrogen-responsive plasma protein expression signature in Atlantic cod (Gadus morhua) revealed by SELDI-TOF MS ' , Ecotoxicology and Environmental Safety , vol. 74 , pp. 2175-2181 . https://doi.org/10.1016/j.ecoenv.2011.07.036 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1016/j.ecoenv.2011.07.036 |
container_title |
Ecotoxicology and Environmental Safety |
container_volume |
74 |
container_issue |
8 |
container_start_page |
2175 |
op_container_end_page |
2181 |
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1766357809234968576 |