From DNA to biomass: opportunities and challenges in species quantification of bulk fisheries products
Fisheries enforcement relies on visual catch identification and quantification at sea or when landed. Silage (fish dissolved in acid) and fish blocks (block frozen fish) are promising methods for on-board processing and storage of low-value catches. We examined the use of nondestructive sampling and...
Published in: | ICES Journal of Marine Science |
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2020
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Online Access: | https://orbit.dtu.dk/en/publications/d4553d0c-67b1-477c-93ed-0d2039e74ff7 https://doi.org/10.1093/icesjms/fsaa115 https://backend.orbit.dtu.dk/ws/files/277295024/Discardless_final_MS.pdf |
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ftdtupubl:oai:pure.atira.dk:publications/d4553d0c-67b1-477c-93ed-0d2039e74ff7 2024-09-15T17:55:33+00:00 From DNA to biomass: opportunities and challenges in species quantification of bulk fisheries products Hansen, Brian Klitgaard Farrant, Gregory Kevin Ogden, Rob Humble, Emily Ólafsdóttir, Guðbjörg Bekkevold, Dorte Knudsen, Steen Wilhelm Møller, Peter Rask Eg Nielsen, Einar 2020 application/pdf https://orbit.dtu.dk/en/publications/d4553d0c-67b1-477c-93ed-0d2039e74ff7 https://doi.org/10.1093/icesjms/fsaa115 https://backend.orbit.dtu.dk/ws/files/277295024/Discardless_final_MS.pdf eng eng https://orbit.dtu.dk/en/publications/d4553d0c-67b1-477c-93ed-0d2039e74ff7 info:eu-repo/semantics/openAccess Hansen , B K , Farrant , G K , Ogden , R , Humble , E , Ólafsdóttir , G , Bekkevold , D , Knudsen , S W , Møller , P R & Eg Nielsen , E 2020 , ' From DNA to biomass: opportunities and challenges in species quantification of bulk fisheries products ' , ICES Journal of Marine Science , vol. 77 , no. 7-8 , fsaa115 , pp. 2557-2566 . https://doi.org/10.1093/icesjms/fsaa115 DNA quantification Metabarcoding MinION Multi-species fisheries products Quantitative PCR /dk/atira/pure/sustainabledevelopmentgoals/life_below_water name=SDG 14 - Life Below Water article 2020 ftdtupubl https://doi.org/10.1093/icesjms/fsaa115 2024-07-08T23:51:45Z Fisheries enforcement relies on visual catch identification and quantification at sea or when landed. Silage (fish dissolved in acid) and fish blocks (block frozen fish) are promising methods for on-board processing and storage of low-value catches. We examined the use of nondestructive sampling and two DNA-based methods, quantitative PCR (qPCR) and metabarcoding, to assess species composition and relative abundance in industrial grade experimental silage and fish blocks. We demonstrate the ability to identify and quantify DNA from fish species in both products. qPCR analysis of small silage samples collected over 21 days detected all target control species. DNA from one species (Atlantic wolffish) was consistently overrepresented while, for three species of gadoids (Atlantic cod, haddock and whiting), the DNA content matched input tissue proportions with high accuracy. qPCR and metabarcoding of fish blocks, sampled as run-off water and exterior swabs, provided consistent species detection, with the highest variance observed in quantification from swab samples. Our analysis shows that DNA-based methods have significant potential as a tool for species identification and quantification of complex on-board-processed seafood products and are readily applicable to taxonomically and morphologically similar fish. There is, however, a need for establishing DNA/weight calibration factors for primary fisheries species. Article in Journal/Newspaper atlantic cod Technical University of Denmark: DTU Orbit ICES Journal of Marine Science 77 7-8 2557 2566 |
institution |
Open Polar |
collection |
Technical University of Denmark: DTU Orbit |
op_collection_id |
ftdtupubl |
language |
English |
topic |
DNA quantification Metabarcoding MinION Multi-species fisheries products Quantitative PCR /dk/atira/pure/sustainabledevelopmentgoals/life_below_water name=SDG 14 - Life Below Water |
spellingShingle |
DNA quantification Metabarcoding MinION Multi-species fisheries products Quantitative PCR /dk/atira/pure/sustainabledevelopmentgoals/life_below_water name=SDG 14 - Life Below Water Hansen, Brian Klitgaard Farrant, Gregory Kevin Ogden, Rob Humble, Emily Ólafsdóttir, Guðbjörg Bekkevold, Dorte Knudsen, Steen Wilhelm Møller, Peter Rask Eg Nielsen, Einar From DNA to biomass: opportunities and challenges in species quantification of bulk fisheries products |
topic_facet |
DNA quantification Metabarcoding MinION Multi-species fisheries products Quantitative PCR /dk/atira/pure/sustainabledevelopmentgoals/life_below_water name=SDG 14 - Life Below Water |
description |
Fisheries enforcement relies on visual catch identification and quantification at sea or when landed. Silage (fish dissolved in acid) and fish blocks (block frozen fish) are promising methods for on-board processing and storage of low-value catches. We examined the use of nondestructive sampling and two DNA-based methods, quantitative PCR (qPCR) and metabarcoding, to assess species composition and relative abundance in industrial grade experimental silage and fish blocks. We demonstrate the ability to identify and quantify DNA from fish species in both products. qPCR analysis of small silage samples collected over 21 days detected all target control species. DNA from one species (Atlantic wolffish) was consistently overrepresented while, for three species of gadoids (Atlantic cod, haddock and whiting), the DNA content matched input tissue proportions with high accuracy. qPCR and metabarcoding of fish blocks, sampled as run-off water and exterior swabs, provided consistent species detection, with the highest variance observed in quantification from swab samples. Our analysis shows that DNA-based methods have significant potential as a tool for species identification and quantification of complex on-board-processed seafood products and are readily applicable to taxonomically and morphologically similar fish. There is, however, a need for establishing DNA/weight calibration factors for primary fisheries species. |
format |
Article in Journal/Newspaper |
author |
Hansen, Brian Klitgaard Farrant, Gregory Kevin Ogden, Rob Humble, Emily Ólafsdóttir, Guðbjörg Bekkevold, Dorte Knudsen, Steen Wilhelm Møller, Peter Rask Eg Nielsen, Einar |
author_facet |
Hansen, Brian Klitgaard Farrant, Gregory Kevin Ogden, Rob Humble, Emily Ólafsdóttir, Guðbjörg Bekkevold, Dorte Knudsen, Steen Wilhelm Møller, Peter Rask Eg Nielsen, Einar |
author_sort |
Hansen, Brian Klitgaard |
title |
From DNA to biomass: opportunities and challenges in species quantification of bulk fisheries products |
title_short |
From DNA to biomass: opportunities and challenges in species quantification of bulk fisheries products |
title_full |
From DNA to biomass: opportunities and challenges in species quantification of bulk fisheries products |
title_fullStr |
From DNA to biomass: opportunities and challenges in species quantification of bulk fisheries products |
title_full_unstemmed |
From DNA to biomass: opportunities and challenges in species quantification of bulk fisheries products |
title_sort |
from dna to biomass: opportunities and challenges in species quantification of bulk fisheries products |
publishDate |
2020 |
url |
https://orbit.dtu.dk/en/publications/d4553d0c-67b1-477c-93ed-0d2039e74ff7 https://doi.org/10.1093/icesjms/fsaa115 https://backend.orbit.dtu.dk/ws/files/277295024/Discardless_final_MS.pdf |
genre |
atlantic cod |
genre_facet |
atlantic cod |
op_source |
Hansen , B K , Farrant , G K , Ogden , R , Humble , E , Ólafsdóttir , G , Bekkevold , D , Knudsen , S W , Møller , P R & Eg Nielsen , E 2020 , ' From DNA to biomass: opportunities and challenges in species quantification of bulk fisheries products ' , ICES Journal of Marine Science , vol. 77 , no. 7-8 , fsaa115 , pp. 2557-2566 . https://doi.org/10.1093/icesjms/fsaa115 |
op_relation |
https://orbit.dtu.dk/en/publications/d4553d0c-67b1-477c-93ed-0d2039e74ff7 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1093/icesjms/fsaa115 |
container_title |
ICES Journal of Marine Science |
container_volume |
77 |
container_issue |
7-8 |
container_start_page |
2557 |
op_container_end_page |
2566 |
_version_ |
1810431816378613760 |