Pseudoalteromonas spp. Serve as Initial Bacterial Attractants in Mesocosms of Coastal Waters but Have Subsequent Antifouling Capacity in Mesocosms and when Embedded in Paint
The purpose of the present study was to determine if the monoculture antifouling effect of several pigmented pseudoalteromonads was retained in in vitro mesocosm systems using natural coastal seawater and when the bacteria were embedded in paint used on surfaces submerged in coastal waters. Pseudoal...
Published in: | Applied and Environmental Microbiology |
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Format: | Article in Journal/Newspaper |
Language: | English |
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2013
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Online Access: | https://orbit.dtu.dk/en/publications/c66958fb-8707-497e-afec-49ce6c36b765 https://doi.org/10.1128/AEM.01987-13 |
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ftdtupubl:oai:pure.atira.dk:publications/c66958fb-8707-497e-afec-49ce6c36b765 2024-02-11T09:56:11+01:00 Pseudoalteromonas spp. Serve as Initial Bacterial Attractants in Mesocosms of Coastal Waters but Have Subsequent Antifouling Capacity in Mesocosms and when Embedded in Paint Bernbom, Nete Ng, Yin Møller, Stefan Gram, Lone 2013 https://orbit.dtu.dk/en/publications/c66958fb-8707-497e-afec-49ce6c36b765 https://doi.org/10.1128/AEM.01987-13 eng eng https://orbit.dtu.dk/en/publications/c66958fb-8707-497e-afec-49ce6c36b765 info:eu-repo/semantics/closedAccess Bernbom , N , Ng , Y , Møller , S & Gram , L 2013 , ' Pseudoalteromonas spp. Serve as Initial Bacterial Attractants in Mesocosms of Coastal Waters but Have Subsequent Antifouling Capacity in Mesocosms and when Embedded in Paint ' , Applied and Environmental Microbiology , vol. 79 , no. 22 , pp. 6885-6893 . https://doi.org/10.1128/AEM.01987-13 article 2013 ftdtupubl https://doi.org/10.1128/AEM.01987-13 2024-01-17T23:57:27Z The purpose of the present study was to determine if the monoculture antifouling effect of several pigmented pseudoalteromonads was retained in in vitro mesocosm systems using natural coastal seawater and when the bacteria were embedded in paint used on surfaces submerged in coastal waters. Pseudoalteromonas piscicida survived on a steel surface and retained antifouling activity for at least 53 days in sterile seawater, whereas P. tunicata survived and had antifouling activity for only 1 week. However, during the first week, all Pseudoalteromonas strains facilitated rather than prevented bacterial attachment when used to coat stainless steel surfaces and submerged in mesocosms with natural seawater. The bacterial density on surfaces coated with sterile growth medium was 105 cells/cm2 after 7 days, whereas counts on surfaces precoated with Pseudoalteromonas were significantly higher, at 106 to 108 cells/cm2. However, after 53 days, seven of eight Pseudoalteromonas strains had reduced total bacterial adhesion compared to the control. P. piscicida, P. antarctica, and P. ulvae remained on the surface, at levels similar to those in the initial coating, whereas P. tunicata could not be detected. Larger fouling organisms were observed on all plates precoated with Pseudoalteromonas; however, plates coated only with sterile growth medium were dominated by a bacterial biofilm. Suspensions of a P. piscicida strain and a P. tunicata strain were incorporated into ship paints (Hempasil x3 87500 and Hempasil 77500) used on plates that were placed at the Hempel A/S test site in Jyllinge Harbor. For the first 4 months, no differences were observed between control plates and treated plates, but after 5 to 6 months, the control plates were more fouled than the plates with pseudoalteromonad-based paint. Our study demonstrates that no single laboratory assay can predict antifouling effects and that a combination of laboratory and real-life methods must be used to determine the potential antifouling capability of new agents or ... Article in Journal/Newspaper Antarc* Antarctica Technical University of Denmark: DTU Orbit Applied and Environmental Microbiology 79 22 6885 6893 |
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Open Polar |
collection |
Technical University of Denmark: DTU Orbit |
op_collection_id |
ftdtupubl |
language |
English |
description |
The purpose of the present study was to determine if the monoculture antifouling effect of several pigmented pseudoalteromonads was retained in in vitro mesocosm systems using natural coastal seawater and when the bacteria were embedded in paint used on surfaces submerged in coastal waters. Pseudoalteromonas piscicida survived on a steel surface and retained antifouling activity for at least 53 days in sterile seawater, whereas P. tunicata survived and had antifouling activity for only 1 week. However, during the first week, all Pseudoalteromonas strains facilitated rather than prevented bacterial attachment when used to coat stainless steel surfaces and submerged in mesocosms with natural seawater. The bacterial density on surfaces coated with sterile growth medium was 105 cells/cm2 after 7 days, whereas counts on surfaces precoated with Pseudoalteromonas were significantly higher, at 106 to 108 cells/cm2. However, after 53 days, seven of eight Pseudoalteromonas strains had reduced total bacterial adhesion compared to the control. P. piscicida, P. antarctica, and P. ulvae remained on the surface, at levels similar to those in the initial coating, whereas P. tunicata could not be detected. Larger fouling organisms were observed on all plates precoated with Pseudoalteromonas; however, plates coated only with sterile growth medium were dominated by a bacterial biofilm. Suspensions of a P. piscicida strain and a P. tunicata strain were incorporated into ship paints (Hempasil x3 87500 and Hempasil 77500) used on plates that were placed at the Hempel A/S test site in Jyllinge Harbor. For the first 4 months, no differences were observed between control plates and treated plates, but after 5 to 6 months, the control plates were more fouled than the plates with pseudoalteromonad-based paint. Our study demonstrates that no single laboratory assay can predict antifouling effects and that a combination of laboratory and real-life methods must be used to determine the potential antifouling capability of new agents or ... |
format |
Article in Journal/Newspaper |
author |
Bernbom, Nete Ng, Yin Møller, Stefan Gram, Lone |
spellingShingle |
Bernbom, Nete Ng, Yin Møller, Stefan Gram, Lone Pseudoalteromonas spp. Serve as Initial Bacterial Attractants in Mesocosms of Coastal Waters but Have Subsequent Antifouling Capacity in Mesocosms and when Embedded in Paint |
author_facet |
Bernbom, Nete Ng, Yin Møller, Stefan Gram, Lone |
author_sort |
Bernbom, Nete |
title |
Pseudoalteromonas spp. Serve as Initial Bacterial Attractants in Mesocosms of Coastal Waters but Have Subsequent Antifouling Capacity in Mesocosms and when Embedded in Paint |
title_short |
Pseudoalteromonas spp. Serve as Initial Bacterial Attractants in Mesocosms of Coastal Waters but Have Subsequent Antifouling Capacity in Mesocosms and when Embedded in Paint |
title_full |
Pseudoalteromonas spp. Serve as Initial Bacterial Attractants in Mesocosms of Coastal Waters but Have Subsequent Antifouling Capacity in Mesocosms and when Embedded in Paint |
title_fullStr |
Pseudoalteromonas spp. Serve as Initial Bacterial Attractants in Mesocosms of Coastal Waters but Have Subsequent Antifouling Capacity in Mesocosms and when Embedded in Paint |
title_full_unstemmed |
Pseudoalteromonas spp. Serve as Initial Bacterial Attractants in Mesocosms of Coastal Waters but Have Subsequent Antifouling Capacity in Mesocosms and when Embedded in Paint |
title_sort |
pseudoalteromonas spp. serve as initial bacterial attractants in mesocosms of coastal waters but have subsequent antifouling capacity in mesocosms and when embedded in paint |
publishDate |
2013 |
url |
https://orbit.dtu.dk/en/publications/c66958fb-8707-497e-afec-49ce6c36b765 https://doi.org/10.1128/AEM.01987-13 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Bernbom , N , Ng , Y , Møller , S & Gram , L 2013 , ' Pseudoalteromonas spp. Serve as Initial Bacterial Attractants in Mesocosms of Coastal Waters but Have Subsequent Antifouling Capacity in Mesocosms and when Embedded in Paint ' , Applied and Environmental Microbiology , vol. 79 , no. 22 , pp. 6885-6893 . https://doi.org/10.1128/AEM.01987-13 |
op_relation |
https://orbit.dtu.dk/en/publications/c66958fb-8707-497e-afec-49ce6c36b765 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1128/AEM.01987-13 |
container_title |
Applied and Environmental Microbiology |
container_volume |
79 |
container_issue |
22 |
container_start_page |
6885 |
op_container_end_page |
6893 |
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1790601233395875840 |