First genetic quantification of sex- and stage-specific feeding in the ubiquitous copepod Acartia tonsa
Marine copepods provide the major food-web link between primary producers and higher trophic levels, and their feeding ecology is of acute interest in light of global change impacts on food-web functioning. Recently, quantitative polymerase chain reaction (qPCR) protocols have been developed, which...
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2018
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Online Access: | https://orbit.dtu.dk/en/publications/c29e7e1f-596f-4dfc-bead-723b9c5064d5 https://doi.org/10.1007/s00227-017-3281-z https://rdcu.be/b1Nlw |
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ftdtupubl:oai:pure.atira.dk:publications/c29e7e1f-596f-4dfc-bead-723b9c5064d5 2024-02-11T10:07:37+01:00 First genetic quantification of sex- and stage-specific feeding in the ubiquitous copepod Acartia tonsa Ismar, Stefanie M.H. Kottmann, Johanna Sarah Sommer, Ulrich 2018 https://orbit.dtu.dk/en/publications/c29e7e1f-596f-4dfc-bead-723b9c5064d5 https://doi.org/10.1007/s00227-017-3281-z https://rdcu.be/b1Nlw eng eng https://orbit.dtu.dk/en/publications/c29e7e1f-596f-4dfc-bead-723b9c5064d5 info:eu-repo/semantics/openAccess Ismar , S M H , Kottmann , J S & Sommer , U 2018 , ' First genetic quantification of sex- and stage-specific feeding in the ubiquitous copepod Acartia tonsa ' , Marine Biology , vol. 165 , no. 2 , 25 . https://doi.org/10.1007/s00227-017-3281-z Ecology Evolution Behavior and Systematics Aquatic Science Acartia Acartia tonsa algae Bacillariophyta Calanoida Copepoda Cryptophyta Isochrysis galbana Rhodomonas (Cryptophyta) Thalassiosira weissflogii Environment Marine & Freshwater Sciences Freshwater & Marine Ecology Oceanography Microbiology Zoology SC7 /dk/atira/pure/sustainabledevelopmentgoals/life_below_water name=SDG 14 - Life Below Water article 2018 ftdtupubl https://doi.org/10.1007/s00227-017-3281-z 2024-01-17T23:58:30Z Marine copepods provide the major food-web link between primary producers and higher trophic levels, and their feeding ecology is of acute interest in light of global change impacts on food-web functioning. Recently, quantitative polymerase chain reaction (qPCR) protocols have been developed, which can complement classic diet quantification methods, such as stable isotope or fatty acid analyses tools. Here, we present first results of feeding experiments assessing sex- and stage-specific food intake by the ubiquitous calanoid copepod Acartia tonsa by 18S targeted qPCR and microscopic grazing assessment. In triplicated mixed-diet feeding treatments, three suitable A. tonsa diets, the cryptophyte Rhodomonas balthica, the haptophyte Isochrysis galbana, and the diatom Thalassiosira weissflogii, were offered in equal biomass proportions under constant conditions. Prey uptake substantially varied between different algal species, as did the extent of sex- and stage-specificity of prey uptake. Male adult copepods had higher R. balthica gut contents than females, and nauplii contained more of this prey source than copepodites or adult copepods in mixed treatments. A trend towards higher amounts of ingested T. weissflogii in adult females than in males and in nauplii than in other stages was detected. Genetic gut content quantifications indicated low feeding on I. galbana, and no consistent sex- or stage-specific differences of I. galbana content in A. tonsa. Our results highlight diet-specific feeding differences between Acartia life stages and sexes, which can have implications on food-web dynamics and specific nutrient transfer to higher trophic levels in copepod populations of varying age composition under changing environmental parameters, such as rising temperatures and increasing ocean acidification. Article in Journal/Newspaper Ocean acidification Copepods Technical University of Denmark: DTU Orbit Marine Biology 165 2 |
institution |
Open Polar |
collection |
Technical University of Denmark: DTU Orbit |
op_collection_id |
ftdtupubl |
language |
English |
topic |
Ecology Evolution Behavior and Systematics Aquatic Science Acartia Acartia tonsa algae Bacillariophyta Calanoida Copepoda Cryptophyta Isochrysis galbana Rhodomonas (Cryptophyta) Thalassiosira weissflogii Environment Marine & Freshwater Sciences Freshwater & Marine Ecology Oceanography Microbiology Zoology SC7 /dk/atira/pure/sustainabledevelopmentgoals/life_below_water name=SDG 14 - Life Below Water |
spellingShingle |
Ecology Evolution Behavior and Systematics Aquatic Science Acartia Acartia tonsa algae Bacillariophyta Calanoida Copepoda Cryptophyta Isochrysis galbana Rhodomonas (Cryptophyta) Thalassiosira weissflogii Environment Marine & Freshwater Sciences Freshwater & Marine Ecology Oceanography Microbiology Zoology SC7 /dk/atira/pure/sustainabledevelopmentgoals/life_below_water name=SDG 14 - Life Below Water Ismar, Stefanie M.H. Kottmann, Johanna Sarah Sommer, Ulrich First genetic quantification of sex- and stage-specific feeding in the ubiquitous copepod Acartia tonsa |
topic_facet |
Ecology Evolution Behavior and Systematics Aquatic Science Acartia Acartia tonsa algae Bacillariophyta Calanoida Copepoda Cryptophyta Isochrysis galbana Rhodomonas (Cryptophyta) Thalassiosira weissflogii Environment Marine & Freshwater Sciences Freshwater & Marine Ecology Oceanography Microbiology Zoology SC7 /dk/atira/pure/sustainabledevelopmentgoals/life_below_water name=SDG 14 - Life Below Water |
description |
Marine copepods provide the major food-web link between primary producers and higher trophic levels, and their feeding ecology is of acute interest in light of global change impacts on food-web functioning. Recently, quantitative polymerase chain reaction (qPCR) protocols have been developed, which can complement classic diet quantification methods, such as stable isotope or fatty acid analyses tools. Here, we present first results of feeding experiments assessing sex- and stage-specific food intake by the ubiquitous calanoid copepod Acartia tonsa by 18S targeted qPCR and microscopic grazing assessment. In triplicated mixed-diet feeding treatments, three suitable A. tonsa diets, the cryptophyte Rhodomonas balthica, the haptophyte Isochrysis galbana, and the diatom Thalassiosira weissflogii, were offered in equal biomass proportions under constant conditions. Prey uptake substantially varied between different algal species, as did the extent of sex- and stage-specificity of prey uptake. Male adult copepods had higher R. balthica gut contents than females, and nauplii contained more of this prey source than copepodites or adult copepods in mixed treatments. A trend towards higher amounts of ingested T. weissflogii in adult females than in males and in nauplii than in other stages was detected. Genetic gut content quantifications indicated low feeding on I. galbana, and no consistent sex- or stage-specific differences of I. galbana content in A. tonsa. Our results highlight diet-specific feeding differences between Acartia life stages and sexes, which can have implications on food-web dynamics and specific nutrient transfer to higher trophic levels in copepod populations of varying age composition under changing environmental parameters, such as rising temperatures and increasing ocean acidification. |
format |
Article in Journal/Newspaper |
author |
Ismar, Stefanie M.H. Kottmann, Johanna Sarah Sommer, Ulrich |
author_facet |
Ismar, Stefanie M.H. Kottmann, Johanna Sarah Sommer, Ulrich |
author_sort |
Ismar, Stefanie M.H. |
title |
First genetic quantification of sex- and stage-specific feeding in the ubiquitous copepod Acartia tonsa |
title_short |
First genetic quantification of sex- and stage-specific feeding in the ubiquitous copepod Acartia tonsa |
title_full |
First genetic quantification of sex- and stage-specific feeding in the ubiquitous copepod Acartia tonsa |
title_fullStr |
First genetic quantification of sex- and stage-specific feeding in the ubiquitous copepod Acartia tonsa |
title_full_unstemmed |
First genetic quantification of sex- and stage-specific feeding in the ubiquitous copepod Acartia tonsa |
title_sort |
first genetic quantification of sex- and stage-specific feeding in the ubiquitous copepod acartia tonsa |
publishDate |
2018 |
url |
https://orbit.dtu.dk/en/publications/c29e7e1f-596f-4dfc-bead-723b9c5064d5 https://doi.org/10.1007/s00227-017-3281-z https://rdcu.be/b1Nlw |
genre |
Ocean acidification Copepods |
genre_facet |
Ocean acidification Copepods |
op_source |
Ismar , S M H , Kottmann , J S & Sommer , U 2018 , ' First genetic quantification of sex- and stage-specific feeding in the ubiquitous copepod Acartia tonsa ' , Marine Biology , vol. 165 , no. 2 , 25 . https://doi.org/10.1007/s00227-017-3281-z |
op_relation |
https://orbit.dtu.dk/en/publications/c29e7e1f-596f-4dfc-bead-723b9c5064d5 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1007/s00227-017-3281-z |
container_title |
Marine Biology |
container_volume |
165 |
container_issue |
2 |
_version_ |
1790606268062236672 |