Inhibition of methane hydrate nucleation and growth by an antifreeze protein
Antifreeze proteins (AFPs) are characterized by their ability to protect organisms from subfreezing temperatures. They constitute a class of promising candidates as environmentally kinetic hydrate inhibitors (KHIs). In this study, the effectiveness of an insect cell expressed novel monomeric strepta...
Published in: | Journal of Petroleum Science and Engineering |
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Online Access: | https://orbit.dtu.dk/en/publications/bacf7cf0-96f5-4323-91cd-46888086c305 https://doi.org/10.1016/j.petrol.2019.106388 https://backend.orbit.dtu.dk/ws/files/189837033/1_s2.0_S0920410519308095_main.pdf |
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ftdtupubl:oai:pure.atira.dk:publications/bacf7cf0-96f5-4323-91cd-46888086c305 2024-09-15T18:18:41+00:00 Inhibition of methane hydrate nucleation and growth by an antifreeze protein Mu, Liang Ramløv, Hans Søgaard, T. Max M. Jørgensen, Thomas de Jongh, Willem A. von Solms, Nicolas 2019 application/pdf https://orbit.dtu.dk/en/publications/bacf7cf0-96f5-4323-91cd-46888086c305 https://doi.org/10.1016/j.petrol.2019.106388 https://backend.orbit.dtu.dk/ws/files/189837033/1_s2.0_S0920410519308095_main.pdf eng eng https://orbit.dtu.dk/en/publications/bacf7cf0-96f5-4323-91cd-46888086c305 info:eu-repo/semantics/openAccess Mu , L , Ramløv , H , Søgaard , T M M , Jørgensen , T , de Jongh , W A & von Solms , N 2019 , ' Inhibition of methane hydrate nucleation and growth by an antifreeze protein ' , Journal of Petroleum Science and Engineering , vol. 183 , 106388 . https://doi.org/10.1016/j.petrol.2019.106388 Hydrate nucleation Inhibition Antifreeze protein Environment friendly article 2019 ftdtupubl https://doi.org/10.1016/j.petrol.2019.106388 2024-06-25T14:53:04Z Antifreeze proteins (AFPs) are characterized by their ability to protect organisms from subfreezing temperatures. They constitute a class of promising candidates as environmentally kinetic hydrate inhibitors (KHIs). In this study, the effectiveness of an insect cell expressed novel monomeric streptavidin fusion protein version of Rhagium mordax RmAFP1 antifreeze protein (mSA-RmAFP1), and four amino acids (histidine, lysine, tyrosine and proline), on CH 4 hydrate nucleation, growth and decomposition was investigated using a rocking cell apparatus, then compared with the commercial inhibitors Polyvinylpyrrolidone (PVP) and Luvicap Bio. It was found that CH 4 hydrate nucleation and growth exhibited good repeatable results under experimental conditions. The results showed that 2250 ppm mSA-RmAFP1 can inhibit CH 4 hydrate nucleation as effectively as PVP at the same concentration. The histidine, lysine, tyrosine and proline exhibited weak inhibition effect on CH 4 hydrate nucleation. The mSA-RmAFP1 decreased CH 4 hydrate growth rate and production in the fresh and memory solutions. The CH 4 hydrate formed in the solutions containing various tested KHIs present slightly lower onset decomposition temperatures than the non-inhibited system under experimental conditions. The promising performance of the insect cell expressed mSA-RmAFP1 could promote the further development of green hydrate inhibitors. The production of this protein through insect cell line fermentation provides a platform for the future production and optimization of AFPs for hydrate inhibition. Article in Journal/Newspaper Methane hydrate Technical University of Denmark: DTU Orbit Journal of Petroleum Science and Engineering 183 106388 |
institution |
Open Polar |
collection |
Technical University of Denmark: DTU Orbit |
op_collection_id |
ftdtupubl |
language |
English |
topic |
Hydrate nucleation Inhibition Antifreeze protein Environment friendly |
spellingShingle |
Hydrate nucleation Inhibition Antifreeze protein Environment friendly Mu, Liang Ramløv, Hans Søgaard, T. Max M. Jørgensen, Thomas de Jongh, Willem A. von Solms, Nicolas Inhibition of methane hydrate nucleation and growth by an antifreeze protein |
topic_facet |
Hydrate nucleation Inhibition Antifreeze protein Environment friendly |
description |
Antifreeze proteins (AFPs) are characterized by their ability to protect organisms from subfreezing temperatures. They constitute a class of promising candidates as environmentally kinetic hydrate inhibitors (KHIs). In this study, the effectiveness of an insect cell expressed novel monomeric streptavidin fusion protein version of Rhagium mordax RmAFP1 antifreeze protein (mSA-RmAFP1), and four amino acids (histidine, lysine, tyrosine and proline), on CH 4 hydrate nucleation, growth and decomposition was investigated using a rocking cell apparatus, then compared with the commercial inhibitors Polyvinylpyrrolidone (PVP) and Luvicap Bio. It was found that CH 4 hydrate nucleation and growth exhibited good repeatable results under experimental conditions. The results showed that 2250 ppm mSA-RmAFP1 can inhibit CH 4 hydrate nucleation as effectively as PVP at the same concentration. The histidine, lysine, tyrosine and proline exhibited weak inhibition effect on CH 4 hydrate nucleation. The mSA-RmAFP1 decreased CH 4 hydrate growth rate and production in the fresh and memory solutions. The CH 4 hydrate formed in the solutions containing various tested KHIs present slightly lower onset decomposition temperatures than the non-inhibited system under experimental conditions. The promising performance of the insect cell expressed mSA-RmAFP1 could promote the further development of green hydrate inhibitors. The production of this protein through insect cell line fermentation provides a platform for the future production and optimization of AFPs for hydrate inhibition. |
format |
Article in Journal/Newspaper |
author |
Mu, Liang Ramløv, Hans Søgaard, T. Max M. Jørgensen, Thomas de Jongh, Willem A. von Solms, Nicolas |
author_facet |
Mu, Liang Ramløv, Hans Søgaard, T. Max M. Jørgensen, Thomas de Jongh, Willem A. von Solms, Nicolas |
author_sort |
Mu, Liang |
title |
Inhibition of methane hydrate nucleation and growth by an antifreeze protein |
title_short |
Inhibition of methane hydrate nucleation and growth by an antifreeze protein |
title_full |
Inhibition of methane hydrate nucleation and growth by an antifreeze protein |
title_fullStr |
Inhibition of methane hydrate nucleation and growth by an antifreeze protein |
title_full_unstemmed |
Inhibition of methane hydrate nucleation and growth by an antifreeze protein |
title_sort |
inhibition of methane hydrate nucleation and growth by an antifreeze protein |
publishDate |
2019 |
url |
https://orbit.dtu.dk/en/publications/bacf7cf0-96f5-4323-91cd-46888086c305 https://doi.org/10.1016/j.petrol.2019.106388 https://backend.orbit.dtu.dk/ws/files/189837033/1_s2.0_S0920410519308095_main.pdf |
genre |
Methane hydrate |
genre_facet |
Methane hydrate |
op_source |
Mu , L , Ramløv , H , Søgaard , T M M , Jørgensen , T , de Jongh , W A & von Solms , N 2019 , ' Inhibition of methane hydrate nucleation and growth by an antifreeze protein ' , Journal of Petroleum Science and Engineering , vol. 183 , 106388 . https://doi.org/10.1016/j.petrol.2019.106388 |
op_relation |
https://orbit.dtu.dk/en/publications/bacf7cf0-96f5-4323-91cd-46888086c305 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1016/j.petrol.2019.106388 |
container_title |
Journal of Petroleum Science and Engineering |
container_volume |
183 |
container_start_page |
106388 |
_version_ |
1810456770345172992 |