Effects of European eel egg and larval stocking density on rearing water, offspring bacteriome and derived immune response
If human exploitation of the “critically endangered” European eel ( Anguilla anguilla ) is to continue, the eel's life cycle in captivity must be closed and techniques established to produce viable offspring in hatcheries. Despite stable offspring production, high mortality rates during early d...
Published in: | Aquaculture |
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Main Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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2024
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Online Access: | https://orbit.dtu.dk/en/publications/b6a9d1df-0093-4e5f-953a-a0289163ad70 https://doi.org/10.1016/j.aquaculture.2024.740716 https://backend.orbit.dtu.dk/ws/files/354650666/1-s2.0-S0966842X23002597-main.pdf |
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ftdtupubl:oai:pure.atira.dk:publications/b6a9d1df-0093-4e5f-953a-a0289163ad70 2024-06-23T07:45:33+00:00 Effects of European eel egg and larval stocking density on rearing water, offspring bacteriome and derived immune response Bandara, Kasun A. Politis, Sebastian N. Sganga, Daniela Eliana Sørensen, Sune Riis Koumpiadis, Paraskevas Tomkiewicz, Jonna Vadstein, Olav 2024 application/pdf https://orbit.dtu.dk/en/publications/b6a9d1df-0093-4e5f-953a-a0289163ad70 https://doi.org/10.1016/j.aquaculture.2024.740716 https://backend.orbit.dtu.dk/ws/files/354650666/1-s2.0-S0966842X23002597-main.pdf eng eng https://orbit.dtu.dk/en/publications/b6a9d1df-0093-4e5f-953a-a0289163ad70 info:eu-repo/semantics/openAccess Bandara , K A , Politis , S N , Sganga , D E , Sørensen , S R , Koumpiadis , P , Tomkiewicz , J & Vadstein , O 2024 , ' Effects of European eel egg and larval stocking density on rearing water, offspring bacteriome and derived immune response ' , Aquaculture , vol. 585 , 740716 . https://doi.org/10.1016/j.aquaculture.2024.740716 Anguilla anguilla Hatchery technology Early life history Micobial interference Immune gene expression /dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being name=SDG 3 - Good Health and Well-being article 2024 ftdtupubl https://doi.org/10.1016/j.aquaculture.2024.740716 2024-06-11T15:20:47Z If human exploitation of the “critically endangered” European eel ( Anguilla anguilla ) is to continue, the eel's life cycle in captivity must be closed and techniques established to produce viable offspring in hatcheries. Despite stable offspring production, high mortality rates during early developmental stages have hindered progress in closing the lifecycle in captivity. We hypothesised that mortalities affecting embryos and newly hatched larvae, when the immune system is expected to play a vital role in protecting the offspring, could be linked to adverse microbial conditions arising in high-density rearing. We therefore set out to explore the bacteriome changes in rearing water and offspring as well as the molecular immunologic development of offspring at different initial stocking densities (500, 1000, 2000, and 4000 eggs/L) from fertilisation to 3 days post-hatch (dph). The lowest density resulted in ~5% higher survival. Independent of density, most mortality (~75%) occurred during embryonic rather than larval development. The bacteriomes of both offspring and rearing water differed according to stocking density, with a potentially healthier bacteriome at the lowest density, marked by greater ASV richness and abundance from various phyla (Proteobacteria, Actinobacteria, Fusobacteria, and Firmicutes). In contrast, the bacteriome of the highest density was dominated by Proteobacteria ASVs, including the potentially harmful Vibrionales order. We observed stage-specific bacteriome changes in offspring, transitioning from an inherent and balanced bacteriome in unadulterated eggs to a dominance of rapid-growing opportunistic bacteria in embryos (48 hpf), and finally to a more diverse bacterial community in larvae (3 dph). Thus, host-microbe interactions appear to significantly impact the overall high mortality observed during embryonic development. As the expression of stress and immune related genes was not affected by density, it seems that the molecular immune system was incapable of handling microbial ... Article in Journal/Newspaper Anguilla anguilla Technical University of Denmark: DTU Orbit Aquaculture 585 740716 |
institution |
Open Polar |
collection |
Technical University of Denmark: DTU Orbit |
op_collection_id |
ftdtupubl |
language |
English |
topic |
Anguilla anguilla Hatchery technology Early life history Micobial interference Immune gene expression /dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being name=SDG 3 - Good Health and Well-being |
spellingShingle |
Anguilla anguilla Hatchery technology Early life history Micobial interference Immune gene expression /dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being name=SDG 3 - Good Health and Well-being Bandara, Kasun A. Politis, Sebastian N. Sganga, Daniela Eliana Sørensen, Sune Riis Koumpiadis, Paraskevas Tomkiewicz, Jonna Vadstein, Olav Effects of European eel egg and larval stocking density on rearing water, offspring bacteriome and derived immune response |
topic_facet |
Anguilla anguilla Hatchery technology Early life history Micobial interference Immune gene expression /dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being name=SDG 3 - Good Health and Well-being |
description |
If human exploitation of the “critically endangered” European eel ( Anguilla anguilla ) is to continue, the eel's life cycle in captivity must be closed and techniques established to produce viable offspring in hatcheries. Despite stable offspring production, high mortality rates during early developmental stages have hindered progress in closing the lifecycle in captivity. We hypothesised that mortalities affecting embryos and newly hatched larvae, when the immune system is expected to play a vital role in protecting the offspring, could be linked to adverse microbial conditions arising in high-density rearing. We therefore set out to explore the bacteriome changes in rearing water and offspring as well as the molecular immunologic development of offspring at different initial stocking densities (500, 1000, 2000, and 4000 eggs/L) from fertilisation to 3 days post-hatch (dph). The lowest density resulted in ~5% higher survival. Independent of density, most mortality (~75%) occurred during embryonic rather than larval development. The bacteriomes of both offspring and rearing water differed according to stocking density, with a potentially healthier bacteriome at the lowest density, marked by greater ASV richness and abundance from various phyla (Proteobacteria, Actinobacteria, Fusobacteria, and Firmicutes). In contrast, the bacteriome of the highest density was dominated by Proteobacteria ASVs, including the potentially harmful Vibrionales order. We observed stage-specific bacteriome changes in offspring, transitioning from an inherent and balanced bacteriome in unadulterated eggs to a dominance of rapid-growing opportunistic bacteria in embryos (48 hpf), and finally to a more diverse bacterial community in larvae (3 dph). Thus, host-microbe interactions appear to significantly impact the overall high mortality observed during embryonic development. As the expression of stress and immune related genes was not affected by density, it seems that the molecular immune system was incapable of handling microbial ... |
format |
Article in Journal/Newspaper |
author |
Bandara, Kasun A. Politis, Sebastian N. Sganga, Daniela Eliana Sørensen, Sune Riis Koumpiadis, Paraskevas Tomkiewicz, Jonna Vadstein, Olav |
author_facet |
Bandara, Kasun A. Politis, Sebastian N. Sganga, Daniela Eliana Sørensen, Sune Riis Koumpiadis, Paraskevas Tomkiewicz, Jonna Vadstein, Olav |
author_sort |
Bandara, Kasun A. |
title |
Effects of European eel egg and larval stocking density on rearing water, offspring bacteriome and derived immune response |
title_short |
Effects of European eel egg and larval stocking density on rearing water, offspring bacteriome and derived immune response |
title_full |
Effects of European eel egg and larval stocking density on rearing water, offspring bacteriome and derived immune response |
title_fullStr |
Effects of European eel egg and larval stocking density on rearing water, offspring bacteriome and derived immune response |
title_full_unstemmed |
Effects of European eel egg and larval stocking density on rearing water, offspring bacteriome and derived immune response |
title_sort |
effects of european eel egg and larval stocking density on rearing water, offspring bacteriome and derived immune response |
publishDate |
2024 |
url |
https://orbit.dtu.dk/en/publications/b6a9d1df-0093-4e5f-953a-a0289163ad70 https://doi.org/10.1016/j.aquaculture.2024.740716 https://backend.orbit.dtu.dk/ws/files/354650666/1-s2.0-S0966842X23002597-main.pdf |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_source |
Bandara , K A , Politis , S N , Sganga , D E , Sørensen , S R , Koumpiadis , P , Tomkiewicz , J & Vadstein , O 2024 , ' Effects of European eel egg and larval stocking density on rearing water, offspring bacteriome and derived immune response ' , Aquaculture , vol. 585 , 740716 . https://doi.org/10.1016/j.aquaculture.2024.740716 |
op_relation |
https://orbit.dtu.dk/en/publications/b6a9d1df-0093-4e5f-953a-a0289163ad70 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1016/j.aquaculture.2024.740716 |
container_title |
Aquaculture |
container_volume |
585 |
container_start_page |
740716 |
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1802640731365441536 |