Improved Protection of Rainbow Trout Against Furunculosis by an Autologous Vaccine Under Experimental Conditions
Despite vaccination with oil-adjuvanted vaccines against vibriosis and furunculosis, sea reared rainbow trout in Denmark often encounter outbreaks of furunculosis during warm summer periods. To address this issue under experimental conditions, two groups of rainbow trout were vaccinated by i.p. inje...
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University of Copenhagen
2012
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ftdtupubl:oai:pure.atira.dk:publications/9d2e1eea-250b-4bac-bb30-d73316247ff1 2024-06-23T07:51:25+00:00 Improved Protection of Rainbow Trout Against Furunculosis by an Autologous Vaccine Under Experimental Conditions Lorenzen, Ellen Kjær, Torben Egil Henriksen, Niels Henrik Dalsgaard, Inger Holten-Andersen, Lars Madsen, S. B. Krossøy, B. Buchmann, Kurt Lorenzen, Niels 2012 https://orbit.dtu.dk/en/publications/9d2e1eea-250b-4bac-bb30-d73316247ff1 http://www.dafinet.dk/DAFINET/Home_files/DAFINET%20November%206-7%202012%20Abstractbook.pdf eng eng University of Copenhagen https://orbit.dtu.dk/en/publications/9d2e1eea-250b-4bac-bb30-d73316247ff1 info:eu-repo/semantics/restrictedAccess Lorenzen , E , Kjær , T E , Henriksen , N H , Dalsgaard , I , Holten-Andersen , L , Madsen , S B , Krossøy , B , Buchmann , K & Lorenzen , N 2012 , Improved Protection of Rainbow Trout Against Furunculosis by an Autologous Vaccine Under Experimental Conditions . in DAFINET Workshop : Immune Responses in Fish : Book of abstracts . University of Copenhagen , pp. 20 , DAFINET Workshop : Immune Responses in Fish , Copenhagen , Denmark , 06/11/2012 . < http://www.dafinet.dk/DAFINET/Home_files/DAFINET%20November%206-7%202012%20Abstractbook.pdf > /dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being name=SDG 3 - Good Health and Well-being conferenceObject 2012 ftdtupubl 2024-06-04T15:02:04Z Despite vaccination with oil-adjuvanted vaccines against vibriosis and furunculosis, sea reared rainbow trout in Denmark often encounter outbreaks of furunculosis during warm summer periods. To address this issue under experimental conditions, two groups of rainbow trout were vaccinated by i.p. injection with two different oil-adjuvanted vaccines: (1) a commercial vaccine comprising Vibrio anguillarum serotype O1 and O2, and Aeromonas salmonicida subspecies salmonicida bacterins, with all bacteria originating from Atlantic salmon, and (2) an experimental vaccine based on cultures of the same bacterial species originating from rainbow trout reared in Danish sea farms. The experiment also included a third group of non-vaccinated controls. All fish were individually chip-tagged to allow mixing of all groups in three replicate aquaria. After 770 dg (day degrees) or 77 days at 10⁰C, half of the fish in each group were challenged by i.p. injection of 1x105 cells of the A. salmonicida isolate used in the experimental vaccine. The other half was tagged by cutting off the adipose fin (non-injected cohabitants). While the non-vaccinated, i.p.-injected fish all died within 2 weeks, a certain level of protection was evident among the vaccinated groups although high mortality also occurred here. No mortality/clinical disease was evident among the non-injected cohabitants. However, when the water temperature was gradually risen to 15-17-20⁰C, the cohabitants started to die. Some variability was evident between replicate tanks, but the experimental vaccine tended to provide better protection than the commercial counterpart. The results indicate that tailor-making of a vaccine against furunculosis for sea reared rainbow trout in DK is an important approach for optimal protection. Conference Object Atlantic salmon Technical University of Denmark: DTU Orbit |
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Technical University of Denmark: DTU Orbit |
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ftdtupubl |
language |
English |
topic |
/dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being name=SDG 3 - Good Health and Well-being |
spellingShingle |
/dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being name=SDG 3 - Good Health and Well-being Lorenzen, Ellen Kjær, Torben Egil Henriksen, Niels Henrik Dalsgaard, Inger Holten-Andersen, Lars Madsen, S. B. Krossøy, B. Buchmann, Kurt Lorenzen, Niels Improved Protection of Rainbow Trout Against Furunculosis by an Autologous Vaccine Under Experimental Conditions |
topic_facet |
/dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being name=SDG 3 - Good Health and Well-being |
description |
Despite vaccination with oil-adjuvanted vaccines against vibriosis and furunculosis, sea reared rainbow trout in Denmark often encounter outbreaks of furunculosis during warm summer periods. To address this issue under experimental conditions, two groups of rainbow trout were vaccinated by i.p. injection with two different oil-adjuvanted vaccines: (1) a commercial vaccine comprising Vibrio anguillarum serotype O1 and O2, and Aeromonas salmonicida subspecies salmonicida bacterins, with all bacteria originating from Atlantic salmon, and (2) an experimental vaccine based on cultures of the same bacterial species originating from rainbow trout reared in Danish sea farms. The experiment also included a third group of non-vaccinated controls. All fish were individually chip-tagged to allow mixing of all groups in three replicate aquaria. After 770 dg (day degrees) or 77 days at 10⁰C, half of the fish in each group were challenged by i.p. injection of 1x105 cells of the A. salmonicida isolate used in the experimental vaccine. The other half was tagged by cutting off the adipose fin (non-injected cohabitants). While the non-vaccinated, i.p.-injected fish all died within 2 weeks, a certain level of protection was evident among the vaccinated groups although high mortality also occurred here. No mortality/clinical disease was evident among the non-injected cohabitants. However, when the water temperature was gradually risen to 15-17-20⁰C, the cohabitants started to die. Some variability was evident between replicate tanks, but the experimental vaccine tended to provide better protection than the commercial counterpart. The results indicate that tailor-making of a vaccine against furunculosis for sea reared rainbow trout in DK is an important approach for optimal protection. |
format |
Conference Object |
author |
Lorenzen, Ellen Kjær, Torben Egil Henriksen, Niels Henrik Dalsgaard, Inger Holten-Andersen, Lars Madsen, S. B. Krossøy, B. Buchmann, Kurt Lorenzen, Niels |
author_facet |
Lorenzen, Ellen Kjær, Torben Egil Henriksen, Niels Henrik Dalsgaard, Inger Holten-Andersen, Lars Madsen, S. B. Krossøy, B. Buchmann, Kurt Lorenzen, Niels |
author_sort |
Lorenzen, Ellen |
title |
Improved Protection of Rainbow Trout Against Furunculosis by an Autologous Vaccine Under Experimental Conditions |
title_short |
Improved Protection of Rainbow Trout Against Furunculosis by an Autologous Vaccine Under Experimental Conditions |
title_full |
Improved Protection of Rainbow Trout Against Furunculosis by an Autologous Vaccine Under Experimental Conditions |
title_fullStr |
Improved Protection of Rainbow Trout Against Furunculosis by an Autologous Vaccine Under Experimental Conditions |
title_full_unstemmed |
Improved Protection of Rainbow Trout Against Furunculosis by an Autologous Vaccine Under Experimental Conditions |
title_sort |
improved protection of rainbow trout against furunculosis by an autologous vaccine under experimental conditions |
publisher |
University of Copenhagen |
publishDate |
2012 |
url |
https://orbit.dtu.dk/en/publications/9d2e1eea-250b-4bac-bb30-d73316247ff1 http://www.dafinet.dk/DAFINET/Home_files/DAFINET%20November%206-7%202012%20Abstractbook.pdf |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Lorenzen , E , Kjær , T E , Henriksen , N H , Dalsgaard , I , Holten-Andersen , L , Madsen , S B , Krossøy , B , Buchmann , K & Lorenzen , N 2012 , Improved Protection of Rainbow Trout Against Furunculosis by an Autologous Vaccine Under Experimental Conditions . in DAFINET Workshop : Immune Responses in Fish : Book of abstracts . University of Copenhagen , pp. 20 , DAFINET Workshop : Immune Responses in Fish , Copenhagen , Denmark , 06/11/2012 . < http://www.dafinet.dk/DAFINET/Home_files/DAFINET%20November%206-7%202012%20Abstractbook.pdf > |
op_relation |
https://orbit.dtu.dk/en/publications/9d2e1eea-250b-4bac-bb30-d73316247ff1 |
op_rights |
info:eu-repo/semantics/restrictedAccess |
_version_ |
1802642517242413056 |