Duplicated leptin receptors in two species of eel bring new insights into the evolution of the leptin system in vertebrates
Since its discovery in mammals as a key-hormone in reproduction and metabolism, leptin has been identified in an increasing number of tetrapods and teleosts. Tetrapods possess only one leptin gene, while most teleosts possess two leptin genes, as a result of the teleost third whole genome duplicatio...
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ftdtupubl:oai:pure.atira.dk:publications/98ba2dab-3f1a-46ee-8a4d-156b61fbb331 2023-08-27T04:09:18+02:00 Duplicated leptin receptors in two species of eel bring new insights into the evolution of the leptin system in vertebrates Morini, M. Pasquier, J. van den Thillart, G. Tomkiewicz, Jonna Rousseau, K. Dufour, S. Lafont, A._G. 2015 application/pdf https://orbit.dtu.dk/en/publications/98ba2dab-3f1a-46ee-8a4d-156b61fbb331 https://doi.org/10.1371/journal.pone.0126008 https://backend.orbit.dtu.dk/ws/files/126599195/journal.pone.0126008.PDF eng eng info:eu-repo/semantics/openAccess Morini , M , Pasquier , J , van den Thillart , G , Tomkiewicz , J , Rousseau , K , Dufour , S & Lafont , A G 2015 , ' Duplicated leptin receptors in two species of eel bring new insights into the evolution of the leptin system in vertebrates ' , PLOS ONE , vol. 10 , no. 5 , e0126008 . https://doi.org/10.1371/journal.pone.0126008 article 2015 ftdtupubl https://doi.org/10.1371/journal.pone.0126008 2023-08-09T22:58:20Z Since its discovery in mammals as a key-hormone in reproduction and metabolism, leptin has been identified in an increasing number of tetrapods and teleosts. Tetrapods possess only one leptin gene, while most teleosts possess two leptin genes, as a result of the teleost third whole genome duplication event (3R). Leptin acts through a specific receptor (LEPR). In the European and Japanese eels, we identified two leptin genes, and for the first time in vertebrates, two LEPR genes. Synteny analyses indicated that eel LEPRa and LEPRb result from teleost 3R. LEPRb seems to have been lost in the teleost lineage shortly after the elopomorph divergence. Quantitative PCRs revealed a wide distribution of leptins and LEPRs in the European eel, including tissues involved in metabolism and reproduction. Noticeably, leptin1 was expressed in fat tissue, while leptin2 in the liver, reflecting subfunctionalization. Four-month fasting had no impact on the expression of leptins and LEPRs in control European eels. This might be related to the remarkable adaptation of silver eel metabolism to long-term fasting throughout the reproductive oceanic migration. In contrast, sexual maturation induced differential increases in the expression of leptins and LEPRs in the BPG-liver axis. Leptin2 was strikingly upregulated in the liver, the central organ of the reproductive metabolic challenge in teleosts. LEPRs were differentially regulated during sexual maturation, which may have contributed to the conservation of the duplicated LEPRs in this species. This suggests an ancient and positive role of the leptin system in the vertebrate reproductive function. This study brings new insights on the evolutionary history of the leptin system in vertebrates. Among extant vertebrates, the eel represents a unique case of duplicated leptins and leptin receptors as a result of 3R Article in Journal/Newspaper European eel Technical University of Denmark: DTU Orbit PLOS ONE 10 5 e0126008 |
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Technical University of Denmark: DTU Orbit |
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ftdtupubl |
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English |
description |
Since its discovery in mammals as a key-hormone in reproduction and metabolism, leptin has been identified in an increasing number of tetrapods and teleosts. Tetrapods possess only one leptin gene, while most teleosts possess two leptin genes, as a result of the teleost third whole genome duplication event (3R). Leptin acts through a specific receptor (LEPR). In the European and Japanese eels, we identified two leptin genes, and for the first time in vertebrates, two LEPR genes. Synteny analyses indicated that eel LEPRa and LEPRb result from teleost 3R. LEPRb seems to have been lost in the teleost lineage shortly after the elopomorph divergence. Quantitative PCRs revealed a wide distribution of leptins and LEPRs in the European eel, including tissues involved in metabolism and reproduction. Noticeably, leptin1 was expressed in fat tissue, while leptin2 in the liver, reflecting subfunctionalization. Four-month fasting had no impact on the expression of leptins and LEPRs in control European eels. This might be related to the remarkable adaptation of silver eel metabolism to long-term fasting throughout the reproductive oceanic migration. In contrast, sexual maturation induced differential increases in the expression of leptins and LEPRs in the BPG-liver axis. Leptin2 was strikingly upregulated in the liver, the central organ of the reproductive metabolic challenge in teleosts. LEPRs were differentially regulated during sexual maturation, which may have contributed to the conservation of the duplicated LEPRs in this species. This suggests an ancient and positive role of the leptin system in the vertebrate reproductive function. This study brings new insights on the evolutionary history of the leptin system in vertebrates. Among extant vertebrates, the eel represents a unique case of duplicated leptins and leptin receptors as a result of 3R |
format |
Article in Journal/Newspaper |
author |
Morini, M. Pasquier, J. van den Thillart, G. Tomkiewicz, Jonna Rousseau, K. Dufour, S. Lafont, A._G. |
spellingShingle |
Morini, M. Pasquier, J. van den Thillart, G. Tomkiewicz, Jonna Rousseau, K. Dufour, S. Lafont, A._G. Duplicated leptin receptors in two species of eel bring new insights into the evolution of the leptin system in vertebrates |
author_facet |
Morini, M. Pasquier, J. van den Thillart, G. Tomkiewicz, Jonna Rousseau, K. Dufour, S. Lafont, A._G. |
author_sort |
Morini, M. |
title |
Duplicated leptin receptors in two species of eel bring new insights into the evolution of the leptin system in vertebrates |
title_short |
Duplicated leptin receptors in two species of eel bring new insights into the evolution of the leptin system in vertebrates |
title_full |
Duplicated leptin receptors in two species of eel bring new insights into the evolution of the leptin system in vertebrates |
title_fullStr |
Duplicated leptin receptors in two species of eel bring new insights into the evolution of the leptin system in vertebrates |
title_full_unstemmed |
Duplicated leptin receptors in two species of eel bring new insights into the evolution of the leptin system in vertebrates |
title_sort |
duplicated leptin receptors in two species of eel bring new insights into the evolution of the leptin system in vertebrates |
publishDate |
2015 |
url |
https://orbit.dtu.dk/en/publications/98ba2dab-3f1a-46ee-8a4d-156b61fbb331 https://doi.org/10.1371/journal.pone.0126008 https://backend.orbit.dtu.dk/ws/files/126599195/journal.pone.0126008.PDF |
genre |
European eel |
genre_facet |
European eel |
op_source |
Morini , M , Pasquier , J , van den Thillart , G , Tomkiewicz , J , Rousseau , K , Dufour , S & Lafont , A G 2015 , ' Duplicated leptin receptors in two species of eel bring new insights into the evolution of the leptin system in vertebrates ' , PLOS ONE , vol. 10 , no. 5 , e0126008 . https://doi.org/10.1371/journal.pone.0126008 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1371/journal.pone.0126008 |
container_title |
PLOS ONE |
container_volume |
10 |
container_issue |
5 |
container_start_page |
e0126008 |
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