Outbreak of viral haemorrhagic septicaemia (VHS) in lumpfish (Cyclopterus lumpus) in Iceland caused by VHS virus genotype IV
A novel viral haemorrhagic septicaemia virus (VHSV) of genotype IV was isolated from wild lumpfish (Cyclopterus lumpus), brought to a land-based farm in Iceland, to serve as broodfish. Two groups of lumpfish juveniles, kept in tanks in the same facility, got infected. The virus isolated was identifi...
Published in: | Journal of Fish Diseases |
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Format: | Article in Journal/Newspaper |
Language: | English |
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2019
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Online Access: | https://orbit.dtu.dk/en/publications/88f1196e-79d8-4af2-852b-90fd283dbf4b https://doi.org/10.1111/jfd.12910 https://backend.orbit.dtu.dk/ws/files/164872986/Postprint.pdf https://backend.orbit.dtu.dk/ws/files/160463035/Publishers_version.pdf |
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ftdtupubl:oai:pure.atira.dk:publications/88f1196e-79d8-4af2-852b-90fd283dbf4b 2024-09-09T19:30:18+00:00 Outbreak of viral haemorrhagic septicaemia (VHS) in lumpfish (Cyclopterus lumpus) in Iceland caused by VHS virus genotype IV Guðmundsdóttir, Sigríður Vendramin, Niccolò Cuenca, Argelia Sigurðardóttir, Heiða Kristmundsson, Arni Moesgaard Iburg, Tine Olesen, Niels Jørgen 2019 application/pdf https://orbit.dtu.dk/en/publications/88f1196e-79d8-4af2-852b-90fd283dbf4b https://doi.org/10.1111/jfd.12910 https://backend.orbit.dtu.dk/ws/files/164872986/Postprint.pdf https://backend.orbit.dtu.dk/ws/files/160463035/Publishers_version.pdf eng eng https://orbit.dtu.dk/en/publications/88f1196e-79d8-4af2-852b-90fd283dbf4b info:eu-repo/semantics/openAccess Guðmundsdóttir , S , Vendramin , N , Cuenca , A , Sigurðardóttir , H , Kristmundsson , A , Moesgaard Iburg , T & Olesen , N J 2019 , ' Outbreak of viral haemorrhagic septicaemia (VHS) in lumpfish (Cyclopterus lumpus) in Iceland caused by VHS virus genotype IV ' , Journal of Fish Diseases , vol. 42 , no. 1 , pp. 47-62 . https://doi.org/10.1111/jfd.12910 Iceland challenge models genotype IV lumpfish viral haemorrhagic septicaemia virus (VHSV) article 2019 ftdtupubl https://doi.org/10.1111/jfd.12910 2024-08-13T00:03:05Z A novel viral haemorrhagic septicaemia virus (VHSV) of genotype IV was isolated from wild lumpfish (Cyclopterus lumpus), brought to a land-based farm in Iceland, to serve as broodfish. Two groups of lumpfish juveniles, kept in tanks in the same facility, got infected. The virus isolated was identified as VHSV by ELISA and real-time RT-PCR. Phylogenetic analysis, based on the glycoprotein (G) gene sequences, may indicate a novel subgroup of VHSV genotype IV. In controlled laboratory exposure studies with this new isolate, there was 3% survival in the I.P. injection challenged group while there was 90% survival in the immersion group. VHSV was not re-isolated from fish challenged by immersion. In a cohabitation trial, lumpfish infected I.P. (shedders) were placed in tanks with naïve lumpfish as well as naïve Atlantic salmon (Salmo salar L.). 10% of the lumpfish shedders and 43%-50% of the cohabiting lumpfish survived after 4 weeks. 80%-92% of the Atlantic salmon survived, but no viral RNA was detected by real-time RT-PCR nor VHSV was isolated from Atlantic salmon. This is the first isolation of a notifiable virus in Iceland and the first report of VHSV of genotype IV in European waters. Article in Journal/Newspaper Atlantic salmon Iceland Salmo salar Technical University of Denmark: DTU Orbit Journal of Fish Diseases 42 1 47 62 |
institution |
Open Polar |
collection |
Technical University of Denmark: DTU Orbit |
op_collection_id |
ftdtupubl |
language |
English |
topic |
Iceland challenge models genotype IV lumpfish viral haemorrhagic septicaemia virus (VHSV) |
spellingShingle |
Iceland challenge models genotype IV lumpfish viral haemorrhagic septicaemia virus (VHSV) Guðmundsdóttir, Sigríður Vendramin, Niccolò Cuenca, Argelia Sigurðardóttir, Heiða Kristmundsson, Arni Moesgaard Iburg, Tine Olesen, Niels Jørgen Outbreak of viral haemorrhagic septicaemia (VHS) in lumpfish (Cyclopterus lumpus) in Iceland caused by VHS virus genotype IV |
topic_facet |
Iceland challenge models genotype IV lumpfish viral haemorrhagic septicaemia virus (VHSV) |
description |
A novel viral haemorrhagic septicaemia virus (VHSV) of genotype IV was isolated from wild lumpfish (Cyclopterus lumpus), brought to a land-based farm in Iceland, to serve as broodfish. Two groups of lumpfish juveniles, kept in tanks in the same facility, got infected. The virus isolated was identified as VHSV by ELISA and real-time RT-PCR. Phylogenetic analysis, based on the glycoprotein (G) gene sequences, may indicate a novel subgroup of VHSV genotype IV. In controlled laboratory exposure studies with this new isolate, there was 3% survival in the I.P. injection challenged group while there was 90% survival in the immersion group. VHSV was not re-isolated from fish challenged by immersion. In a cohabitation trial, lumpfish infected I.P. (shedders) were placed in tanks with naïve lumpfish as well as naïve Atlantic salmon (Salmo salar L.). 10% of the lumpfish shedders and 43%-50% of the cohabiting lumpfish survived after 4 weeks. 80%-92% of the Atlantic salmon survived, but no viral RNA was detected by real-time RT-PCR nor VHSV was isolated from Atlantic salmon. This is the first isolation of a notifiable virus in Iceland and the first report of VHSV of genotype IV in European waters. |
format |
Article in Journal/Newspaper |
author |
Guðmundsdóttir, Sigríður Vendramin, Niccolò Cuenca, Argelia Sigurðardóttir, Heiða Kristmundsson, Arni Moesgaard Iburg, Tine Olesen, Niels Jørgen |
author_facet |
Guðmundsdóttir, Sigríður Vendramin, Niccolò Cuenca, Argelia Sigurðardóttir, Heiða Kristmundsson, Arni Moesgaard Iburg, Tine Olesen, Niels Jørgen |
author_sort |
Guðmundsdóttir, Sigríður |
title |
Outbreak of viral haemorrhagic septicaemia (VHS) in lumpfish (Cyclopterus lumpus) in Iceland caused by VHS virus genotype IV |
title_short |
Outbreak of viral haemorrhagic septicaemia (VHS) in lumpfish (Cyclopterus lumpus) in Iceland caused by VHS virus genotype IV |
title_full |
Outbreak of viral haemorrhagic septicaemia (VHS) in lumpfish (Cyclopterus lumpus) in Iceland caused by VHS virus genotype IV |
title_fullStr |
Outbreak of viral haemorrhagic septicaemia (VHS) in lumpfish (Cyclopterus lumpus) in Iceland caused by VHS virus genotype IV |
title_full_unstemmed |
Outbreak of viral haemorrhagic septicaemia (VHS) in lumpfish (Cyclopterus lumpus) in Iceland caused by VHS virus genotype IV |
title_sort |
outbreak of viral haemorrhagic septicaemia (vhs) in lumpfish (cyclopterus lumpus) in iceland caused by vhs virus genotype iv |
publishDate |
2019 |
url |
https://orbit.dtu.dk/en/publications/88f1196e-79d8-4af2-852b-90fd283dbf4b https://doi.org/10.1111/jfd.12910 https://backend.orbit.dtu.dk/ws/files/164872986/Postprint.pdf https://backend.orbit.dtu.dk/ws/files/160463035/Publishers_version.pdf |
genre |
Atlantic salmon Iceland Salmo salar |
genre_facet |
Atlantic salmon Iceland Salmo salar |
op_source |
Guðmundsdóttir , S , Vendramin , N , Cuenca , A , Sigurðardóttir , H , Kristmundsson , A , Moesgaard Iburg , T & Olesen , N J 2019 , ' Outbreak of viral haemorrhagic septicaemia (VHS) in lumpfish (Cyclopterus lumpus) in Iceland caused by VHS virus genotype IV ' , Journal of Fish Diseases , vol. 42 , no. 1 , pp. 47-62 . https://doi.org/10.1111/jfd.12910 |
op_relation |
https://orbit.dtu.dk/en/publications/88f1196e-79d8-4af2-852b-90fd283dbf4b |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1111/jfd.12910 |
container_title |
Journal of Fish Diseases |
container_volume |
42 |
container_issue |
1 |
container_start_page |
47 |
op_container_end_page |
62 |
_version_ |
1809899289246171136 |