Improved Hydrolysis of Granular Starches by a Psychrophilic α-Amylase Starch Binding Domain-Fusion
Degradation of starch granules by a psychrophilic α-amylase, AHA, from the Antarctic bacterium Pseudoalteromonas haloplanktis TAB23 was facilitated by C-terminal fusion to a starch-binding domain (SBD) from either Aspergillus niger glucoamylase (SBD GA ) or Arabidopsis thaliana glucan, water dikinas...
Published in: | Journal of Agricultural and Food Chemistry |
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Format: | Article in Journal/Newspaper |
Language: | English |
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2023
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Online Access: | https://orbit.dtu.dk/en/publications/7f425908-feb7-495c-9f4d-18ef9b583c4f https://doi.org/10.1021/acs.jafc.3c01898 https://backend.orbit.dtu.dk/ws/files/345932384/Post_print_Improved_Hydrolysis_of_Granular_Starches_by_a_Psychrophilic_-Amylase_Starch_Binding_Domain-Fusion.pdf |
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ftdtupubl:oai:pure.atira.dk:publications/7f425908-feb7-495c-9f4d-18ef9b583c4f 2024-09-15T17:48:38+00:00 Improved Hydrolysis of Granular Starches by a Psychrophilic α-Amylase Starch Binding Domain-Fusion Wang, Yu Tian, Yu Zhong, Yuyue Suleiman, Mohammad Amer Feller, Georges Westh, Peter Blennow, Andreas Møller, Marie Sofie Svensson, Birte 2023 application/pdf https://orbit.dtu.dk/en/publications/7f425908-feb7-495c-9f4d-18ef9b583c4f https://doi.org/10.1021/acs.jafc.3c01898 https://backend.orbit.dtu.dk/ws/files/345932384/Post_print_Improved_Hydrolysis_of_Granular_Starches_by_a_Psychrophilic_-Amylase_Starch_Binding_Domain-Fusion.pdf eng eng https://orbit.dtu.dk/en/publications/7f425908-feb7-495c-9f4d-18ef9b583c4f info:eu-repo/semantics/openAccess Wang , Y , Tian , Y , Zhong , Y , Suleiman , M A , Feller , G , Westh , P , Blennow , A , Møller , M S & Svensson , B 2023 , ' Improved Hydrolysis of Granular Starches by a Psychrophilic α-Amylase Starch Binding Domain-Fusion ' , Journal of Agricultural and Food Chemistry , pp. 9040-9050 . https://doi.org/10.1021/acs.jafc.3c01898 Carbohydrate-binding module Waxy starch Normal starch High-amylose starch Pseudoalteromonas haloplanktis α-amylase Heterogenous catalysis Sabatier principle article 2023 ftdtupubl https://doi.org/10.1021/acs.jafc.3c01898 2024-08-13T00:03:06Z Degradation of starch granules by a psychrophilic α-amylase, AHA, from the Antarctic bacterium Pseudoalteromonas haloplanktis TAB23 was facilitated by C-terminal fusion to a starch-binding domain (SBD) from either Aspergillus niger glucoamylase (SBD GA ) or Arabidopsis thaliana glucan, water dikinase 3 (SBD GWD3 ) via a decapeptide linker. Depending on the waxy, normal or high-amylose starch type and the botanical source, the AHA-SBD fusion enzymes showed up to 3 times higher activity than AHA wild-type. The SBD-fusion thus increased the density of enzyme attack-sites and binding-sites on the starch granules by up to 5- and 7-fold, respectively, as measured using an interfacial catalysis approach that combined conventional Michaelis-Menten kinetics, with the substrate in excess, and inverse kinetics, having enzyme in excess, with enzyme-starch granule adsorption isotherms. Higher substrate affinity of the SBD GA compared to SBD GWD3 was accompanied by the superior activity of AHA-SBD GA in agreement with the Sabatier principle of adsorption limited heterogenous catalysis. Article in Journal/Newspaper Antarc* Antarctic Technical University of Denmark: DTU Orbit Journal of Agricultural and Food Chemistry 71 23 9040 9050 |
institution |
Open Polar |
collection |
Technical University of Denmark: DTU Orbit |
op_collection_id |
ftdtupubl |
language |
English |
topic |
Carbohydrate-binding module Waxy starch Normal starch High-amylose starch Pseudoalteromonas haloplanktis α-amylase Heterogenous catalysis Sabatier principle |
spellingShingle |
Carbohydrate-binding module Waxy starch Normal starch High-amylose starch Pseudoalteromonas haloplanktis α-amylase Heterogenous catalysis Sabatier principle Wang, Yu Tian, Yu Zhong, Yuyue Suleiman, Mohammad Amer Feller, Georges Westh, Peter Blennow, Andreas Møller, Marie Sofie Svensson, Birte Improved Hydrolysis of Granular Starches by a Psychrophilic α-Amylase Starch Binding Domain-Fusion |
topic_facet |
Carbohydrate-binding module Waxy starch Normal starch High-amylose starch Pseudoalteromonas haloplanktis α-amylase Heterogenous catalysis Sabatier principle |
description |
Degradation of starch granules by a psychrophilic α-amylase, AHA, from the Antarctic bacterium Pseudoalteromonas haloplanktis TAB23 was facilitated by C-terminal fusion to a starch-binding domain (SBD) from either Aspergillus niger glucoamylase (SBD GA ) or Arabidopsis thaliana glucan, water dikinase 3 (SBD GWD3 ) via a decapeptide linker. Depending on the waxy, normal or high-amylose starch type and the botanical source, the AHA-SBD fusion enzymes showed up to 3 times higher activity than AHA wild-type. The SBD-fusion thus increased the density of enzyme attack-sites and binding-sites on the starch granules by up to 5- and 7-fold, respectively, as measured using an interfacial catalysis approach that combined conventional Michaelis-Menten kinetics, with the substrate in excess, and inverse kinetics, having enzyme in excess, with enzyme-starch granule adsorption isotherms. Higher substrate affinity of the SBD GA compared to SBD GWD3 was accompanied by the superior activity of AHA-SBD GA in agreement with the Sabatier principle of adsorption limited heterogenous catalysis. |
format |
Article in Journal/Newspaper |
author |
Wang, Yu Tian, Yu Zhong, Yuyue Suleiman, Mohammad Amer Feller, Georges Westh, Peter Blennow, Andreas Møller, Marie Sofie Svensson, Birte |
author_facet |
Wang, Yu Tian, Yu Zhong, Yuyue Suleiman, Mohammad Amer Feller, Georges Westh, Peter Blennow, Andreas Møller, Marie Sofie Svensson, Birte |
author_sort |
Wang, Yu |
title |
Improved Hydrolysis of Granular Starches by a Psychrophilic α-Amylase Starch Binding Domain-Fusion |
title_short |
Improved Hydrolysis of Granular Starches by a Psychrophilic α-Amylase Starch Binding Domain-Fusion |
title_full |
Improved Hydrolysis of Granular Starches by a Psychrophilic α-Amylase Starch Binding Domain-Fusion |
title_fullStr |
Improved Hydrolysis of Granular Starches by a Psychrophilic α-Amylase Starch Binding Domain-Fusion |
title_full_unstemmed |
Improved Hydrolysis of Granular Starches by a Psychrophilic α-Amylase Starch Binding Domain-Fusion |
title_sort |
improved hydrolysis of granular starches by a psychrophilic α-amylase starch binding domain-fusion |
publishDate |
2023 |
url |
https://orbit.dtu.dk/en/publications/7f425908-feb7-495c-9f4d-18ef9b583c4f https://doi.org/10.1021/acs.jafc.3c01898 https://backend.orbit.dtu.dk/ws/files/345932384/Post_print_Improved_Hydrolysis_of_Granular_Starches_by_a_Psychrophilic_-Amylase_Starch_Binding_Domain-Fusion.pdf |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Wang , Y , Tian , Y , Zhong , Y , Suleiman , M A , Feller , G , Westh , P , Blennow , A , Møller , M S & Svensson , B 2023 , ' Improved Hydrolysis of Granular Starches by a Psychrophilic α-Amylase Starch Binding Domain-Fusion ' , Journal of Agricultural and Food Chemistry , pp. 9040-9050 . https://doi.org/10.1021/acs.jafc.3c01898 |
op_relation |
https://orbit.dtu.dk/en/publications/7f425908-feb7-495c-9f4d-18ef9b583c4f |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1021/acs.jafc.3c01898 |
container_title |
Journal of Agricultural and Food Chemistry |
container_volume |
71 |
container_issue |
23 |
container_start_page |
9040 |
op_container_end_page |
9050 |
_version_ |
1810290078341136384 |