Kinetic model of biodiesel production using immobilized lipase Candida antarctica lipase B
We have designed a kinetic model of biodiesel production using Novozym 435 (Nz435) with immobilized Candida antarctica lipase B (CALB) as a catalyst. The scheme assumed reversibility of all reaction steps and imitated phase effects by introducing various molecular species of water and methanol. The...
Published in: | Journal of Molecular Catalysis B: Enzymatic |
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ftdtupubl:oai:pure.atira.dk:publications/6e4e5fd5-9269-48a7-b9f6-ea2326b9ab71 2024-04-28T08:02:18+00:00 Kinetic model of biodiesel production using immobilized lipase Candida antarctica lipase B Fedosov, Sergey Brask, Jesper Pedersen, Anders K. Nordblad, Mathias Woodley, John Xu, Xuebing 2013 https://orbit.dtu.dk/en/publications/6e4e5fd5-9269-48a7-b9f6-ea2326b9ab71 https://doi.org/10.1016/j.molcatb.2012.09.011 eng eng https://orbit.dtu.dk/en/publications/6e4e5fd5-9269-48a7-b9f6-ea2326b9ab71 info:eu-repo/semantics/closedAccess Fedosov , S , Brask , J , Pedersen , A K , Nordblad , M , Woodley , J & Xu , X 2013 , ' Kinetic model of biodiesel production using immobilized lipase Candida antarctica lipase B ' , Journal of Molecular Catalysis B: Enzymatic , vol. 85-86 , pp. 156-168 . https://doi.org/10.1016/j.molcatb.2012.09.011 Biodiesel Enzyme Lipase Kinetic Modeling article 2013 ftdtupubl https://doi.org/10.1016/j.molcatb.2012.09.011 2024-04-03T15:08:16Z We have designed a kinetic model of biodiesel production using Novozym 435 (Nz435) with immobilized Candida antarctica lipase B (CALB) as a catalyst. The scheme assumed reversibility of all reaction steps and imitated phase effects by introducing various molecular species of water and methanol. The global model was assembled from separate reaction blocks analyzed independently. Computer simulations helped to explore behavior of the reaction system under different conditions. It was found that methanolysis of refined oil by CALB is slow, because triglycerides (T) are the least reactive substrates. Conversion to 95% requires 1.5–6 days of incubation depending on the temperature, enzyme concentration, glycerol inhibition, etc. Other substrates, free fatty acids (F), diglycerides (D) and monoglycerides (M), are utilized much faster (1–2h). This means that waste oil is a better feedstock for CALB. Residual enzymatic activity in biodiesel of standard quality causes increase of D above its specification level because of the reaction 2M↔D+G. Filtration or alkaline treatment of the product prior to storage resolves this problem. The optimal field of Nz435 application appears to be decrease of F, M, D in waste oil before the conventional alkaline conversion. Up to 30-fold reduction of F-content can be achieved in 1–2h, and the residual enzyme (if any) does not survive the following alkaline treatment. Article in Journal/Newspaper Antarc* Antarctica Technical University of Denmark: DTU Orbit Journal of Molecular Catalysis B: Enzymatic 85-86 156 168 |
institution |
Open Polar |
collection |
Technical University of Denmark: DTU Orbit |
op_collection_id |
ftdtupubl |
language |
English |
topic |
Biodiesel Enzyme Lipase Kinetic Modeling |
spellingShingle |
Biodiesel Enzyme Lipase Kinetic Modeling Fedosov, Sergey Brask, Jesper Pedersen, Anders K. Nordblad, Mathias Woodley, John Xu, Xuebing Kinetic model of biodiesel production using immobilized lipase Candida antarctica lipase B |
topic_facet |
Biodiesel Enzyme Lipase Kinetic Modeling |
description |
We have designed a kinetic model of biodiesel production using Novozym 435 (Nz435) with immobilized Candida antarctica lipase B (CALB) as a catalyst. The scheme assumed reversibility of all reaction steps and imitated phase effects by introducing various molecular species of water and methanol. The global model was assembled from separate reaction blocks analyzed independently. Computer simulations helped to explore behavior of the reaction system under different conditions. It was found that methanolysis of refined oil by CALB is slow, because triglycerides (T) are the least reactive substrates. Conversion to 95% requires 1.5–6 days of incubation depending on the temperature, enzyme concentration, glycerol inhibition, etc. Other substrates, free fatty acids (F), diglycerides (D) and monoglycerides (M), are utilized much faster (1–2h). This means that waste oil is a better feedstock for CALB. Residual enzymatic activity in biodiesel of standard quality causes increase of D above its specification level because of the reaction 2M↔D+G. Filtration or alkaline treatment of the product prior to storage resolves this problem. The optimal field of Nz435 application appears to be decrease of F, M, D in waste oil before the conventional alkaline conversion. Up to 30-fold reduction of F-content can be achieved in 1–2h, and the residual enzyme (if any) does not survive the following alkaline treatment. |
format |
Article in Journal/Newspaper |
author |
Fedosov, Sergey Brask, Jesper Pedersen, Anders K. Nordblad, Mathias Woodley, John Xu, Xuebing |
author_facet |
Fedosov, Sergey Brask, Jesper Pedersen, Anders K. Nordblad, Mathias Woodley, John Xu, Xuebing |
author_sort |
Fedosov, Sergey |
title |
Kinetic model of biodiesel production using immobilized lipase Candida antarctica lipase B |
title_short |
Kinetic model of biodiesel production using immobilized lipase Candida antarctica lipase B |
title_full |
Kinetic model of biodiesel production using immobilized lipase Candida antarctica lipase B |
title_fullStr |
Kinetic model of biodiesel production using immobilized lipase Candida antarctica lipase B |
title_full_unstemmed |
Kinetic model of biodiesel production using immobilized lipase Candida antarctica lipase B |
title_sort |
kinetic model of biodiesel production using immobilized lipase candida antarctica lipase b |
publishDate |
2013 |
url |
https://orbit.dtu.dk/en/publications/6e4e5fd5-9269-48a7-b9f6-ea2326b9ab71 https://doi.org/10.1016/j.molcatb.2012.09.011 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Fedosov , S , Brask , J , Pedersen , A K , Nordblad , M , Woodley , J & Xu , X 2013 , ' Kinetic model of biodiesel production using immobilized lipase Candida antarctica lipase B ' , Journal of Molecular Catalysis B: Enzymatic , vol. 85-86 , pp. 156-168 . https://doi.org/10.1016/j.molcatb.2012.09.011 |
op_relation |
https://orbit.dtu.dk/en/publications/6e4e5fd5-9269-48a7-b9f6-ea2326b9ab71 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1016/j.molcatb.2012.09.011 |
container_title |
Journal of Molecular Catalysis B: Enzymatic |
container_volume |
85-86 |
container_start_page |
156 |
op_container_end_page |
168 |
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1797573697175814144 |