Extraction and characterization of candidate bioactive compounds in different tissues from salmon (Salmo salar)
There is an interest in bioprospecting organisms from the aquatic environment to find novel bioactive compounds with health promoting or other functional properties. The aim of this study was to evaluate extracts from untreated and heat-treated salmon tissues for their radical scavenging activities...
Main Authors: | , , , , |
---|---|
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2014
|
Subjects: | |
Online Access: | https://orbit.dtu.dk/en/publications/6bf28ae1-28d2-4b82-86ae-948e94ee9908 https://backend.orbit.dtu.dk/ws/files/107916073/250_938_1_PB.pdf http://www.ijarnp.org/index.php/ijarnp/article/view/250 |
id |
ftdtupubl:oai:pure.atira.dk:publications/6bf28ae1-28d2-4b82-86ae-948e94ee9908 |
---|---|
record_format |
openpolar |
spelling |
ftdtupubl:oai:pure.atira.dk:publications/6bf28ae1-28d2-4b82-86ae-948e94ee9908 2023-08-27T04:11:44+02:00 Extraction and characterization of candidate bioactive compounds in different tissues from salmon (Salmo salar) Falkenberg, Susan Skanderup Mikalsen, S. O. Joensen, H. Stagsted, J. Nielsen, Henrik Hauch 2014 application/pdf https://orbit.dtu.dk/en/publications/6bf28ae1-28d2-4b82-86ae-948e94ee9908 https://backend.orbit.dtu.dk/ws/files/107916073/250_938_1_PB.pdf http://www.ijarnp.org/index.php/ijarnp/article/view/250 eng eng info:eu-repo/semantics/openAccess Falkenberg , S S , Mikalsen , S O , Joensen , H , Stagsted , J & Nielsen , H H 2014 , ' Extraction and characterization of candidate bioactive compounds in different tissues from salmon (Salmo salar) ' , International Journal of Applied Research in Natural Products , vol. 7 , no. 4 , pp. 11-25 . < http://www.ijarnp.org/index.php/ijarnp/article/view/250 > Marine by-products Natural compounds Radical scavenging Salmon tissue /dk/atira/pure/sustainabledevelopmentgoals/life_below_water SDG 14 - Life Below Water article 2014 ftdtupubl 2023-08-02T22:56:47Z There is an interest in bioprospecting organisms from the aquatic environment to find novel bioactive compounds with health promoting or other functional properties. The aim of this study was to evaluate extracts from untreated and heat-treated salmon tissues for their radical scavenging activities and for their ability to inhibit activity of the proteases angiotensin I-converting enzyme (ACE) and dipeptidyl peptidase 4 (DPP-4). In vitro assays were used to detect these activities and the corresponding candidate bioactive compounds were characterized by LC-MS/MS.Radical scavenging activity was detected in <10kDa extracts of gills, belly flap muscle and skin with EC50 values of 39, 82 and 100 µg/mL, respectively. No ACE or DPP-4 inhibiting activity could be detected. LC-MS/MS analysis of dominating compounds in active fractions from size exclusion chromatography showed that families of related compounds were found in several fractions from different tissues but most pronounced in gills. One family was defined according to content of a specific amino acid sequence (PW). Three families were defined by the m/z value of the smallest compound reported in each family (219, 434 and 403). The three latter families did not contain standard unmodified amino acids, indicating peptides with modified amino acids or other kinds of molecules.Industrial relevance. Bioprospecting in fish tissue traditionally regarded as waste can lead to detection of novel natural bioactive compounds including peptides, which could have nutritional, pharmaceutical or other functional value and be used in health and functional foods, thus increasing the value adding of secondary marine products. A number of naturally occurring antimicrobial peptides have been characterized from fish skin and gills, such as piscidins, but these and other fish tissues may contain numerous other compounds with bioactive properties. Such compounds could be extracted by the subsection of the fish industry that processes marine secondary products and further ... Article in Journal/Newspaper Salmo salar Technical University of Denmark: DTU Orbit |
institution |
Open Polar |
collection |
Technical University of Denmark: DTU Orbit |
op_collection_id |
ftdtupubl |
language |
English |
topic |
Marine by-products Natural compounds Radical scavenging Salmon tissue /dk/atira/pure/sustainabledevelopmentgoals/life_below_water SDG 14 - Life Below Water |
spellingShingle |
Marine by-products Natural compounds Radical scavenging Salmon tissue /dk/atira/pure/sustainabledevelopmentgoals/life_below_water SDG 14 - Life Below Water Falkenberg, Susan Skanderup Mikalsen, S. O. Joensen, H. Stagsted, J. Nielsen, Henrik Hauch Extraction and characterization of candidate bioactive compounds in different tissues from salmon (Salmo salar) |
topic_facet |
Marine by-products Natural compounds Radical scavenging Salmon tissue /dk/atira/pure/sustainabledevelopmentgoals/life_below_water SDG 14 - Life Below Water |
description |
There is an interest in bioprospecting organisms from the aquatic environment to find novel bioactive compounds with health promoting or other functional properties. The aim of this study was to evaluate extracts from untreated and heat-treated salmon tissues for their radical scavenging activities and for their ability to inhibit activity of the proteases angiotensin I-converting enzyme (ACE) and dipeptidyl peptidase 4 (DPP-4). In vitro assays were used to detect these activities and the corresponding candidate bioactive compounds were characterized by LC-MS/MS.Radical scavenging activity was detected in <10kDa extracts of gills, belly flap muscle and skin with EC50 values of 39, 82 and 100 µg/mL, respectively. No ACE or DPP-4 inhibiting activity could be detected. LC-MS/MS analysis of dominating compounds in active fractions from size exclusion chromatography showed that families of related compounds were found in several fractions from different tissues but most pronounced in gills. One family was defined according to content of a specific amino acid sequence (PW). Three families were defined by the m/z value of the smallest compound reported in each family (219, 434 and 403). The three latter families did not contain standard unmodified amino acids, indicating peptides with modified amino acids or other kinds of molecules.Industrial relevance. Bioprospecting in fish tissue traditionally regarded as waste can lead to detection of novel natural bioactive compounds including peptides, which could have nutritional, pharmaceutical or other functional value and be used in health and functional foods, thus increasing the value adding of secondary marine products. A number of naturally occurring antimicrobial peptides have been characterized from fish skin and gills, such as piscidins, but these and other fish tissues may contain numerous other compounds with bioactive properties. Such compounds could be extracted by the subsection of the fish industry that processes marine secondary products and further ... |
format |
Article in Journal/Newspaper |
author |
Falkenberg, Susan Skanderup Mikalsen, S. O. Joensen, H. Stagsted, J. Nielsen, Henrik Hauch |
author_facet |
Falkenberg, Susan Skanderup Mikalsen, S. O. Joensen, H. Stagsted, J. Nielsen, Henrik Hauch |
author_sort |
Falkenberg, Susan Skanderup |
title |
Extraction and characterization of candidate bioactive compounds in different tissues from salmon (Salmo salar) |
title_short |
Extraction and characterization of candidate bioactive compounds in different tissues from salmon (Salmo salar) |
title_full |
Extraction and characterization of candidate bioactive compounds in different tissues from salmon (Salmo salar) |
title_fullStr |
Extraction and characterization of candidate bioactive compounds in different tissues from salmon (Salmo salar) |
title_full_unstemmed |
Extraction and characterization of candidate bioactive compounds in different tissues from salmon (Salmo salar) |
title_sort |
extraction and characterization of candidate bioactive compounds in different tissues from salmon (salmo salar) |
publishDate |
2014 |
url |
https://orbit.dtu.dk/en/publications/6bf28ae1-28d2-4b82-86ae-948e94ee9908 https://backend.orbit.dtu.dk/ws/files/107916073/250_938_1_PB.pdf http://www.ijarnp.org/index.php/ijarnp/article/view/250 |
genre |
Salmo salar |
genre_facet |
Salmo salar |
op_source |
Falkenberg , S S , Mikalsen , S O , Joensen , H , Stagsted , J & Nielsen , H H 2014 , ' Extraction and characterization of candidate bioactive compounds in different tissues from salmon (Salmo salar) ' , International Journal of Applied Research in Natural Products , vol. 7 , no. 4 , pp. 11-25 . < http://www.ijarnp.org/index.php/ijarnp/article/view/250 > |
op_rights |
info:eu-repo/semantics/openAccess |
_version_ |
1775354808669044736 |