Effect of TDA-producing Phaeobacter inhibens on the fish pathogen Vibrio anguillarum in non-axenic algae and copepod systems
The expanding aquaculture industry plays an important role in feeding the growing human population and with the expansion, sustainable bacterial disease control, such as probiotics, becomes increasingly important. Tropodithietic acid (TDA)‐producing Phaeobacter spp. can protect live feed, for exampl...
Published in: | Microbial Biotechnology |
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2018
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Online Access: | https://orbit.dtu.dk/en/publications/6b615576-66fe-48bd-b52b-934b6c3c01cc https://doi.org/10.1111/1751-7915.13275 https://backend.orbit.dtu.dk/ws/files/158214729/Rasmussen_et_al_2018_Microbial_Biotechnology.pdf |
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ftdtupubl:oai:pure.atira.dk:publications/6b615576-66fe-48bd-b52b-934b6c3c01cc 2024-05-19T07:49:43+00:00 Effect of TDA-producing Phaeobacter inhibens on the fish pathogen Vibrio anguillarum in non-axenic algae and copepod systems Rasmussen, Bastian Barker Erner, Katrine Ege Bentzon-Tilia, Mikkel Gram, Lone 2018 application/pdf https://orbit.dtu.dk/en/publications/6b615576-66fe-48bd-b52b-934b6c3c01cc https://doi.org/10.1111/1751-7915.13275 https://backend.orbit.dtu.dk/ws/files/158214729/Rasmussen_et_al_2018_Microbial_Biotechnology.pdf eng eng https://orbit.dtu.dk/en/publications/6b615576-66fe-48bd-b52b-934b6c3c01cc info:eu-repo/semantics/openAccess Rasmussen , B B , Erner , K E , Bentzon-Tilia , M & Gram , L 2018 , ' Effect of TDA-producing Phaeobacter inhibens on the fish pathogen Vibrio anguillarum in non-axenic algae and copepod systems ' , Microbial Biotechnology , vol. 11 , no. 6 , pp. 1070-1079 . https://doi.org/10.1111/1751-7915.13275 /dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being name=SDG 3 - Good Health and Well-being article 2018 ftdtupubl https://doi.org/10.1111/1751-7915.13275 2024-04-24T00:44:21Z The expanding aquaculture industry plays an important role in feeding the growing human population and with the expansion, sustainable bacterial disease control, such as probiotics, becomes increasingly important. Tropodithietic acid (TDA)‐producing Phaeobacter spp. can protect live feed, for example rotifers and Artemia as well as larvae of turbot and cod against pathogenic vibrios. Here, we show that the emerging live feed, copepods, is unaffected by colonization of the fish pathogen Vibrio anguillarum , making them potential infection vectors. However, TDA‐producing Phaeobacter inhibens was able to significantly inhibit V. anguillarum in non‐axenic cultures of copepod Acartia tonsa and the copepod feed Rhodomonas salina . Vibrio grew to 10 6 CFU ml −1 and 10 7 CFU ml −1 in copepod and R. salina cultures, respectively. However, vibrio counts remained at the inoculum level (10 4 CFU ml −1 ) when P. inhibens was also added. We further developed a semi‐strain‐specific qPCR for V. anguillarum to detect and quantify the pathogen in non‐axenic systems. In conclusion, P. inhibens efficiently inhibits the fish larval pathogen V. anguillarum in the emerging live feed, copepods, supporting its use as a probiotic in aquaculture. Furthermore, qPCR provides an effective method for detecting vibrio pathogens in complex non‐axenic live feed systems. Article in Journal/Newspaper Turbot Copepods Technical University of Denmark: DTU Orbit Microbial Biotechnology 11 6 1070 1079 |
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Open Polar |
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Technical University of Denmark: DTU Orbit |
op_collection_id |
ftdtupubl |
language |
English |
topic |
/dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being name=SDG 3 - Good Health and Well-being |
spellingShingle |
/dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being name=SDG 3 - Good Health and Well-being Rasmussen, Bastian Barker Erner, Katrine Ege Bentzon-Tilia, Mikkel Gram, Lone Effect of TDA-producing Phaeobacter inhibens on the fish pathogen Vibrio anguillarum in non-axenic algae and copepod systems |
topic_facet |
/dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being name=SDG 3 - Good Health and Well-being |
description |
The expanding aquaculture industry plays an important role in feeding the growing human population and with the expansion, sustainable bacterial disease control, such as probiotics, becomes increasingly important. Tropodithietic acid (TDA)‐producing Phaeobacter spp. can protect live feed, for example rotifers and Artemia as well as larvae of turbot and cod against pathogenic vibrios. Here, we show that the emerging live feed, copepods, is unaffected by colonization of the fish pathogen Vibrio anguillarum , making them potential infection vectors. However, TDA‐producing Phaeobacter inhibens was able to significantly inhibit V. anguillarum in non‐axenic cultures of copepod Acartia tonsa and the copepod feed Rhodomonas salina . Vibrio grew to 10 6 CFU ml −1 and 10 7 CFU ml −1 in copepod and R. salina cultures, respectively. However, vibrio counts remained at the inoculum level (10 4 CFU ml −1 ) when P. inhibens was also added. We further developed a semi‐strain‐specific qPCR for V. anguillarum to detect and quantify the pathogen in non‐axenic systems. In conclusion, P. inhibens efficiently inhibits the fish larval pathogen V. anguillarum in the emerging live feed, copepods, supporting its use as a probiotic in aquaculture. Furthermore, qPCR provides an effective method for detecting vibrio pathogens in complex non‐axenic live feed systems. |
format |
Article in Journal/Newspaper |
author |
Rasmussen, Bastian Barker Erner, Katrine Ege Bentzon-Tilia, Mikkel Gram, Lone |
author_facet |
Rasmussen, Bastian Barker Erner, Katrine Ege Bentzon-Tilia, Mikkel Gram, Lone |
author_sort |
Rasmussen, Bastian Barker |
title |
Effect of TDA-producing Phaeobacter inhibens on the fish pathogen Vibrio anguillarum in non-axenic algae and copepod systems |
title_short |
Effect of TDA-producing Phaeobacter inhibens on the fish pathogen Vibrio anguillarum in non-axenic algae and copepod systems |
title_full |
Effect of TDA-producing Phaeobacter inhibens on the fish pathogen Vibrio anguillarum in non-axenic algae and copepod systems |
title_fullStr |
Effect of TDA-producing Phaeobacter inhibens on the fish pathogen Vibrio anguillarum in non-axenic algae and copepod systems |
title_full_unstemmed |
Effect of TDA-producing Phaeobacter inhibens on the fish pathogen Vibrio anguillarum in non-axenic algae and copepod systems |
title_sort |
effect of tda-producing phaeobacter inhibens on the fish pathogen vibrio anguillarum in non-axenic algae and copepod systems |
publishDate |
2018 |
url |
https://orbit.dtu.dk/en/publications/6b615576-66fe-48bd-b52b-934b6c3c01cc https://doi.org/10.1111/1751-7915.13275 https://backend.orbit.dtu.dk/ws/files/158214729/Rasmussen_et_al_2018_Microbial_Biotechnology.pdf |
genre |
Turbot Copepods |
genre_facet |
Turbot Copepods |
op_source |
Rasmussen , B B , Erner , K E , Bentzon-Tilia , M & Gram , L 2018 , ' Effect of TDA-producing Phaeobacter inhibens on the fish pathogen Vibrio anguillarum in non-axenic algae and copepod systems ' , Microbial Biotechnology , vol. 11 , no. 6 , pp. 1070-1079 . https://doi.org/10.1111/1751-7915.13275 |
op_relation |
https://orbit.dtu.dk/en/publications/6b615576-66fe-48bd-b52b-934b6c3c01cc |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1111/1751-7915.13275 |
container_title |
Microbial Biotechnology |
container_volume |
11 |
container_issue |
6 |
container_start_page |
1070 |
op_container_end_page |
1079 |
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1799468252821192704 |