Biofunctionality of Enzymatically Derived Peptides from Codfish (Gadus morhua) Frame: Bulk In Vitro Properties, Quantitative Proteomics, and Bioinformatic Prediction
Protein hydrolysates show great promise as bioactive food and feed ingredients and for valorization of side-streams from e.g., the fish processing industry. We present a novel approach for hydrolysate characterization that utilizes proteomics data for calculation of weighted mean peptide properties...
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ftdtupubl:oai:pure.atira.dk:publications/556f7cac-d5e3-4dd0-9277-b2b9303d19ea 2024-09-09T19:40:56+00:00 Biofunctionality of Enzymatically Derived Peptides from Codfish (Gadus morhua) Frame: Bulk In Vitro Properties, Quantitative Proteomics, and Bioinformatic Prediction Jafarpour, Ali Gregersen, Simon Marciel Gomes, Rocio Marcatili, Paolo Olsen, Tobias Hegelund Jacobsen, Charlotte Overgaard, Michael Toft Sørensen, Ann-Dorit Moltke 2020 application/pdf https://orbit.dtu.dk/en/publications/556f7cac-d5e3-4dd0-9277-b2b9303d19ea https://doi.org/10.3390/md18120599 https://backend.orbit.dtu.dk/ws/files/235390389/marinedrugs_18_00599.pdf eng eng https://orbit.dtu.dk/en/publications/556f7cac-d5e3-4dd0-9277-b2b9303d19ea info:eu-repo/semantics/openAccess Jafarpour , A , Gregersen , S , Marciel Gomes , R , Marcatili , P , Olsen , T H , Jacobsen , C , Overgaard , M T & Sørensen , A-D M 2020 , ' Biofunctionality of Enzymatically Derived Peptides from Codfish (Gadus morhua) Frame: Bulk In Vitro Properties, Quantitative Proteomics, and Bioinformatic Prediction ' , Marine Drugs , vol. 18 , no. 12 , 599 . https://doi.org/10.3390/md18120599 article 2020 ftdtupubl https://doi.org/10.3390/md18120599 2024-06-18T14:52:18Z Protein hydrolysates show great promise as bioactive food and feed ingredients and for valorization of side-streams from e.g., the fish processing industry. We present a novel approach for hydrolysate characterization that utilizes proteomics data for calculation of weighted mean peptide properties (length, molecular weight, and charge) and peptide-level abundance estimation. Using a novel bioinformatic approach for subsequent prediction of biofunctional properties of identified peptides, we are able to provide an unprecedented, in-depth characterization. The study further characterizes bulk emulsifying, foaming, and in vitro antioxidative properties of enzymatic hydrolysates derived from cod frame by application of Alcalase and Neutrase, individually and sequentially, as well as the influence of heat pre-treatment. All hydrolysates displayed comparable or higher emulsifying activity and stability than sodium caseinate. Heat-treatment significantly increased stability but showed a negative effect on the activity and degree of hydrolysis. Lower degrees of hydrolysis resulted in significantly higher chelating activity, while the opposite was observed for radical scavenging activity. Combining peptide abundance with bioinformatic prediction, we identified several peptides that are likely linked to the observed differences in bulk emulsifying properties. The study highlights the prospects of applying proteomics and bioinformatics for hydrolysate characterization and in food protein science. Article in Journal/Newspaper Gadus morhua Technical University of Denmark: DTU Orbit Marine Drugs 18 12 599 |
institution |
Open Polar |
collection |
Technical University of Denmark: DTU Orbit |
op_collection_id |
ftdtupubl |
language |
English |
description |
Protein hydrolysates show great promise as bioactive food and feed ingredients and for valorization of side-streams from e.g., the fish processing industry. We present a novel approach for hydrolysate characterization that utilizes proteomics data for calculation of weighted mean peptide properties (length, molecular weight, and charge) and peptide-level abundance estimation. Using a novel bioinformatic approach for subsequent prediction of biofunctional properties of identified peptides, we are able to provide an unprecedented, in-depth characterization. The study further characterizes bulk emulsifying, foaming, and in vitro antioxidative properties of enzymatic hydrolysates derived from cod frame by application of Alcalase and Neutrase, individually and sequentially, as well as the influence of heat pre-treatment. All hydrolysates displayed comparable or higher emulsifying activity and stability than sodium caseinate. Heat-treatment significantly increased stability but showed a negative effect on the activity and degree of hydrolysis. Lower degrees of hydrolysis resulted in significantly higher chelating activity, while the opposite was observed for radical scavenging activity. Combining peptide abundance with bioinformatic prediction, we identified several peptides that are likely linked to the observed differences in bulk emulsifying properties. The study highlights the prospects of applying proteomics and bioinformatics for hydrolysate characterization and in food protein science. |
format |
Article in Journal/Newspaper |
author |
Jafarpour, Ali Gregersen, Simon Marciel Gomes, Rocio Marcatili, Paolo Olsen, Tobias Hegelund Jacobsen, Charlotte Overgaard, Michael Toft Sørensen, Ann-Dorit Moltke |
spellingShingle |
Jafarpour, Ali Gregersen, Simon Marciel Gomes, Rocio Marcatili, Paolo Olsen, Tobias Hegelund Jacobsen, Charlotte Overgaard, Michael Toft Sørensen, Ann-Dorit Moltke Biofunctionality of Enzymatically Derived Peptides from Codfish (Gadus morhua) Frame: Bulk In Vitro Properties, Quantitative Proteomics, and Bioinformatic Prediction |
author_facet |
Jafarpour, Ali Gregersen, Simon Marciel Gomes, Rocio Marcatili, Paolo Olsen, Tobias Hegelund Jacobsen, Charlotte Overgaard, Michael Toft Sørensen, Ann-Dorit Moltke |
author_sort |
Jafarpour, Ali |
title |
Biofunctionality of Enzymatically Derived Peptides from Codfish (Gadus morhua) Frame: Bulk In Vitro Properties, Quantitative Proteomics, and Bioinformatic Prediction |
title_short |
Biofunctionality of Enzymatically Derived Peptides from Codfish (Gadus morhua) Frame: Bulk In Vitro Properties, Quantitative Proteomics, and Bioinformatic Prediction |
title_full |
Biofunctionality of Enzymatically Derived Peptides from Codfish (Gadus morhua) Frame: Bulk In Vitro Properties, Quantitative Proteomics, and Bioinformatic Prediction |
title_fullStr |
Biofunctionality of Enzymatically Derived Peptides from Codfish (Gadus morhua) Frame: Bulk In Vitro Properties, Quantitative Proteomics, and Bioinformatic Prediction |
title_full_unstemmed |
Biofunctionality of Enzymatically Derived Peptides from Codfish (Gadus morhua) Frame: Bulk In Vitro Properties, Quantitative Proteomics, and Bioinformatic Prediction |
title_sort |
biofunctionality of enzymatically derived peptides from codfish (gadus morhua) frame: bulk in vitro properties, quantitative proteomics, and bioinformatic prediction |
publishDate |
2020 |
url |
https://orbit.dtu.dk/en/publications/556f7cac-d5e3-4dd0-9277-b2b9303d19ea https://doi.org/10.3390/md18120599 https://backend.orbit.dtu.dk/ws/files/235390389/marinedrugs_18_00599.pdf |
genre |
Gadus morhua |
genre_facet |
Gadus morhua |
op_source |
Jafarpour , A , Gregersen , S , Marciel Gomes , R , Marcatili , P , Olsen , T H , Jacobsen , C , Overgaard , M T & Sørensen , A-D M 2020 , ' Biofunctionality of Enzymatically Derived Peptides from Codfish (Gadus morhua) Frame: Bulk In Vitro Properties, Quantitative Proteomics, and Bioinformatic Prediction ' , Marine Drugs , vol. 18 , no. 12 , 599 . https://doi.org/10.3390/md18120599 |
op_relation |
https://orbit.dtu.dk/en/publications/556f7cac-d5e3-4dd0-9277-b2b9303d19ea |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.3390/md18120599 |
container_title |
Marine Drugs |
container_volume |
18 |
container_issue |
12 |
container_start_page |
599 |
_version_ |
1809910411666915328 |