Effects of ambient versus reduced UV-B radiation on high arctic Salix arctica assessed by measurements and calculations of chlorophyll-a fluorescence parameters from fluorescence transients
A UV-B exclusion-experiment was conducted in the high arctic Zackenberg, NE Greenland, in which Salix arctica leaves during most of the growing season were fixed perpendicular to the solar zenith angle, thereby receiving maximal solar radiation. Covered with Teflon and Mylar foil, the leaves receive...
Published in: | Physiologia Plantarum |
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Language: | English |
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2005
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Online Access: | https://orbit.dtu.dk/en/publications/4c14d004-7b41-4f60-90b1-9095ab9e9e8d https://doi.org/10.1111/j.1399-3054.2005.00502.x https://backend.orbit.dtu.dk/ws/files/4005375/paper_Albert2005_ppl.pdf |
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ftdtupubl:oai:pure.atira.dk:publications/4c14d004-7b41-4f60-90b1-9095ab9e9e8d 2024-01-14T10:03:12+01:00 Effects of ambient versus reduced UV-B radiation on high arctic Salix arctica assessed by measurements and calculations of chlorophyll-a fluorescence parameters from fluorescence transients Albert, K.R. Mikkelsen, Teis Nørgaard Ro-Poulsen, H. 2005 application/pdf https://orbit.dtu.dk/en/publications/4c14d004-7b41-4f60-90b1-9095ab9e9e8d https://doi.org/10.1111/j.1399-3054.2005.00502.x https://backend.orbit.dtu.dk/ws/files/4005375/paper_Albert2005_ppl.pdf eng eng https://orbit.dtu.dk/en/publications/4c14d004-7b41-4f60-90b1-9095ab9e9e8d info:eu-repo/semantics/openAccess Albert , K R , Mikkelsen , T N & Ro-Poulsen , H 2005 , ' Effects of ambient versus reduced UV-B radiation on high arctic Salix arctica assessed by measurements and calculations of chlorophyll-a fluorescence parameters from fluorescence transients ' , Physiologia Plantarum , vol. 124 , no. 2 , pp. 208-226 . https://doi.org/10.1111/j.1399-3054.2005.00502.x 9-B risiko article 2005 ftdtupubl https://doi.org/10.1111/j.1399-3054.2005.00502.x 2023-12-20T23:56:56Z A UV-B exclusion-experiment was conducted in the high arctic Zackenberg, NE Greenland, in which Salix arctica leaves during most of the growing season were fixed perpendicular to the solar zenith angle, thereby receiving maximal solar radiation. Covered with Teflon and Mylar foil, the leaves received approximately 90 and 40% of the ambient UV-B irradiance, respectively. The effects were examined through recordings of chlorophyll a fluorescence transients, determination of biomass and analysis of total carbon and nitrogen content and amount of soluble flavonoids in the leaves. The processing of light was analysed by means of the chlorophyll a fluorescence transient, using the so-called JIP test, as evolved by Reto J. Strasser and his coworkers. Reduction of the UV-B irradiance caused a rise in many of the fluorescence parameters during July, but not in August (late season). Thus increases in the efficiency that an absorbed photon will be trapped by the PSII reaction centre with the resultant reduction of QA to QA – (ET0/ABS = FV/FM) and the efficiency that an electron residing on QA – will enter the intersystem electron transport chain (ET0/TR0) were observed in reduced UV-B. Moreover, estimated per cross-section of leaf sample, the number of active PSII reaction centres (RC/ CSM) and electron transport rate (ETM/CSM) and all performance indexes (PIABS, PICSo and PICSm) were increased in reduced UV-B. The total soluble flavonoid content was highest in ambient UV-B. The treatment effects on fluorescence parameters that were directly measured (e.g. F0 and FM) and those that were derived (e.g. quantum efficiencies, parameters per PSII reaction centres and per cross-section of leaf sample) are discussed in relation to one another, in relation to daily and seasonal variation, and from the perspective of evaluating the relative importance of UV-B of donor and acceptor side capacity in Photosystem II. In conclusion, the experimental set-up and non-invasive measurements proved to be a sensitive method to screen for ... Article in Journal/Newspaper Arctic Arctic Greenland Zackenberg Technical University of Denmark: DTU Orbit Arctic Greenland Physiologia Plantarum 124 2 208 226 |
institution |
Open Polar |
collection |
Technical University of Denmark: DTU Orbit |
op_collection_id |
ftdtupubl |
language |
English |
topic |
9-B risiko |
spellingShingle |
9-B risiko Albert, K.R. Mikkelsen, Teis Nørgaard Ro-Poulsen, H. Effects of ambient versus reduced UV-B radiation on high arctic Salix arctica assessed by measurements and calculations of chlorophyll-a fluorescence parameters from fluorescence transients |
topic_facet |
9-B risiko |
description |
A UV-B exclusion-experiment was conducted in the high arctic Zackenberg, NE Greenland, in which Salix arctica leaves during most of the growing season were fixed perpendicular to the solar zenith angle, thereby receiving maximal solar radiation. Covered with Teflon and Mylar foil, the leaves received approximately 90 and 40% of the ambient UV-B irradiance, respectively. The effects were examined through recordings of chlorophyll a fluorescence transients, determination of biomass and analysis of total carbon and nitrogen content and amount of soluble flavonoids in the leaves. The processing of light was analysed by means of the chlorophyll a fluorescence transient, using the so-called JIP test, as evolved by Reto J. Strasser and his coworkers. Reduction of the UV-B irradiance caused a rise in many of the fluorescence parameters during July, but not in August (late season). Thus increases in the efficiency that an absorbed photon will be trapped by the PSII reaction centre with the resultant reduction of QA to QA – (ET0/ABS = FV/FM) and the efficiency that an electron residing on QA – will enter the intersystem electron transport chain (ET0/TR0) were observed in reduced UV-B. Moreover, estimated per cross-section of leaf sample, the number of active PSII reaction centres (RC/ CSM) and electron transport rate (ETM/CSM) and all performance indexes (PIABS, PICSo and PICSm) were increased in reduced UV-B. The total soluble flavonoid content was highest in ambient UV-B. The treatment effects on fluorescence parameters that were directly measured (e.g. F0 and FM) and those that were derived (e.g. quantum efficiencies, parameters per PSII reaction centres and per cross-section of leaf sample) are discussed in relation to one another, in relation to daily and seasonal variation, and from the perspective of evaluating the relative importance of UV-B of donor and acceptor side capacity in Photosystem II. In conclusion, the experimental set-up and non-invasive measurements proved to be a sensitive method to screen for ... |
format |
Article in Journal/Newspaper |
author |
Albert, K.R. Mikkelsen, Teis Nørgaard Ro-Poulsen, H. |
author_facet |
Albert, K.R. Mikkelsen, Teis Nørgaard Ro-Poulsen, H. |
author_sort |
Albert, K.R. |
title |
Effects of ambient versus reduced UV-B radiation on high arctic Salix arctica assessed by measurements and calculations of chlorophyll-a fluorescence parameters from fluorescence transients |
title_short |
Effects of ambient versus reduced UV-B radiation on high arctic Salix arctica assessed by measurements and calculations of chlorophyll-a fluorescence parameters from fluorescence transients |
title_full |
Effects of ambient versus reduced UV-B radiation on high arctic Salix arctica assessed by measurements and calculations of chlorophyll-a fluorescence parameters from fluorescence transients |
title_fullStr |
Effects of ambient versus reduced UV-B radiation on high arctic Salix arctica assessed by measurements and calculations of chlorophyll-a fluorescence parameters from fluorescence transients |
title_full_unstemmed |
Effects of ambient versus reduced UV-B radiation on high arctic Salix arctica assessed by measurements and calculations of chlorophyll-a fluorescence parameters from fluorescence transients |
title_sort |
effects of ambient versus reduced uv-b radiation on high arctic salix arctica assessed by measurements and calculations of chlorophyll-a fluorescence parameters from fluorescence transients |
publishDate |
2005 |
url |
https://orbit.dtu.dk/en/publications/4c14d004-7b41-4f60-90b1-9095ab9e9e8d https://doi.org/10.1111/j.1399-3054.2005.00502.x https://backend.orbit.dtu.dk/ws/files/4005375/paper_Albert2005_ppl.pdf |
geographic |
Arctic Greenland |
geographic_facet |
Arctic Greenland |
genre |
Arctic Arctic Greenland Zackenberg |
genre_facet |
Arctic Arctic Greenland Zackenberg |
op_source |
Albert , K R , Mikkelsen , T N & Ro-Poulsen , H 2005 , ' Effects of ambient versus reduced UV-B radiation on high arctic Salix arctica assessed by measurements and calculations of chlorophyll-a fluorescence parameters from fluorescence transients ' , Physiologia Plantarum , vol. 124 , no. 2 , pp. 208-226 . https://doi.org/10.1111/j.1399-3054.2005.00502.x |
op_relation |
https://orbit.dtu.dk/en/publications/4c14d004-7b41-4f60-90b1-9095ab9e9e8d |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1111/j.1399-3054.2005.00502.x |
container_title |
Physiologia Plantarum |
container_volume |
124 |
container_issue |
2 |
container_start_page |
208 |
op_container_end_page |
226 |
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1788058125529513984 |