Species-specific detection and quantification of environmental DNA from marine fishes in the Baltic Sea
Biomass assesment of fish stocks is a difficult task that often involves costly fisheries trawl surveys. Trawl surveys rarely return replicate samples, as trawling attempts are expensive and difficult to reproduce. Furthermore, traditional benthic trawling is often detrimental to habitats and the or...
Published in: | Journal of Experimental Marine Biology and Ecology |
---|---|
Main Authors: | , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2019
|
Subjects: | |
Online Access: | https://orbit.dtu.dk/en/publications/34f27ee8-83df-407a-b872-0b8f5782d483 https://doi.org/10.1016/j.jembe.2018.09.004 |
id |
ftdtupubl:oai:pure.atira.dk:publications/34f27ee8-83df-407a-b872-0b8f5782d483 |
---|---|
record_format |
openpolar |
spelling |
ftdtupubl:oai:pure.atira.dk:publications/34f27ee8-83df-407a-b872-0b8f5782d483 2024-06-23T07:45:34+00:00 Species-specific detection and quantification of environmental DNA from marine fishes in the Baltic Sea Knudsen, Steen Wilhelm Ebert, Rasmus Bach Hesselsøe, Martin Kuntke, Franziska Hassingboe, Jakob Mortensen, Peter Bondgaard Thomsen, Philip Francis Sigsgaard, Eva Egelyng Hansen, Brian Klitgaard Eg Nielsen, Einar Møller, Peter Rask 2019 https://orbit.dtu.dk/en/publications/34f27ee8-83df-407a-b872-0b8f5782d483 https://doi.org/10.1016/j.jembe.2018.09.004 eng eng https://orbit.dtu.dk/en/publications/34f27ee8-83df-407a-b872-0b8f5782d483 info:eu-repo/semantics/closedAccess Knudsen , S W , Ebert , R B , Hesselsøe , M , Kuntke , F , Hassingboe , J , Mortensen , P B , Thomsen , P F , Sigsgaard , E E , Hansen , B K , Eg Nielsen , E & Møller , P R 2019 , ' Species-specific detection and quantification of environmental DNA from marine fishes in the Baltic Sea ' , Journal of Experimental Marine Biology and Ecology , vol. 510 , pp. 31-45 . https://doi.org/10.1016/j.jembe.2018.09.004 eDNA Fish stock monitoring North Eastern Atlantic Ocean qPCR /dk/atira/pure/sustainabledevelopmentgoals/life_below_water name=SDG 14 - Life Below Water article 2019 ftdtupubl https://doi.org/10.1016/j.jembe.2018.09.004 2024-06-11T14:52:03Z Biomass assesment of fish stocks is a difficult task that often involves costly fisheries trawl surveys. Trawl surveys rarely return replicate samples, as trawling attempts are expensive and difficult to reproduce. Furthermore, traditional benthic trawling is often detrimental to habitats and the organisms associated with the sea bottom. The rapidly developing field of environmental DNA (eDNA) analysis offers a new approach to non-invasive monitoring of fish. In the present study, we develop and test species-specific primers and probes for qPCR detection of eDNA from Atlantic herring (Clupea harengus), Atlantic cod (Gadus morhua), European flounder (Platichthys flesus), European plaice (Pleuronectes platessa), and Atlantic mackerel (Scomber scombrus) in the Baltic Sea. A recently published qPCR system was applied for European eel (Anguilla anguilla). Filtered water samples were collected during a stratified benthic trawl survey, enabling parallel comparisons of eDNA concentrations with biomass caught by trawling. No significant correlation was found between eDNA concentrations and the biomass of fish caught by the trawl, although an association was observed between the measured concentrations of eDNA and the known distributions and main abundances of cod, herring, plaice and flounder. This indicates that while eDNA concentrations may not be directly comparable to results from existing methods – likely because aquatic eDNA concentrations are not controlled by the same combinations of factors as e.g. trawl biomass catch – eDNA analysis could prove a useful supplement for monitoring fish stocks in the future Article in Journal/Newspaper Anguilla anguilla atlantic cod European eel Gadus morhua Technical University of Denmark: DTU Orbit Journal of Experimental Marine Biology and Ecology 510 31 45 |
institution |
Open Polar |
collection |
Technical University of Denmark: DTU Orbit |
op_collection_id |
ftdtupubl |
language |
English |
topic |
eDNA Fish stock monitoring North Eastern Atlantic Ocean qPCR /dk/atira/pure/sustainabledevelopmentgoals/life_below_water name=SDG 14 - Life Below Water |
spellingShingle |
eDNA Fish stock monitoring North Eastern Atlantic Ocean qPCR /dk/atira/pure/sustainabledevelopmentgoals/life_below_water name=SDG 14 - Life Below Water Knudsen, Steen Wilhelm Ebert, Rasmus Bach Hesselsøe, Martin Kuntke, Franziska Hassingboe, Jakob Mortensen, Peter Bondgaard Thomsen, Philip Francis Sigsgaard, Eva Egelyng Hansen, Brian Klitgaard Eg Nielsen, Einar Møller, Peter Rask Species-specific detection and quantification of environmental DNA from marine fishes in the Baltic Sea |
topic_facet |
eDNA Fish stock monitoring North Eastern Atlantic Ocean qPCR /dk/atira/pure/sustainabledevelopmentgoals/life_below_water name=SDG 14 - Life Below Water |
description |
Biomass assesment of fish stocks is a difficult task that often involves costly fisheries trawl surveys. Trawl surveys rarely return replicate samples, as trawling attempts are expensive and difficult to reproduce. Furthermore, traditional benthic trawling is often detrimental to habitats and the organisms associated with the sea bottom. The rapidly developing field of environmental DNA (eDNA) analysis offers a new approach to non-invasive monitoring of fish. In the present study, we develop and test species-specific primers and probes for qPCR detection of eDNA from Atlantic herring (Clupea harengus), Atlantic cod (Gadus morhua), European flounder (Platichthys flesus), European plaice (Pleuronectes platessa), and Atlantic mackerel (Scomber scombrus) in the Baltic Sea. A recently published qPCR system was applied for European eel (Anguilla anguilla). Filtered water samples were collected during a stratified benthic trawl survey, enabling parallel comparisons of eDNA concentrations with biomass caught by trawling. No significant correlation was found between eDNA concentrations and the biomass of fish caught by the trawl, although an association was observed between the measured concentrations of eDNA and the known distributions and main abundances of cod, herring, plaice and flounder. This indicates that while eDNA concentrations may not be directly comparable to results from existing methods – likely because aquatic eDNA concentrations are not controlled by the same combinations of factors as e.g. trawl biomass catch – eDNA analysis could prove a useful supplement for monitoring fish stocks in the future |
format |
Article in Journal/Newspaper |
author |
Knudsen, Steen Wilhelm Ebert, Rasmus Bach Hesselsøe, Martin Kuntke, Franziska Hassingboe, Jakob Mortensen, Peter Bondgaard Thomsen, Philip Francis Sigsgaard, Eva Egelyng Hansen, Brian Klitgaard Eg Nielsen, Einar Møller, Peter Rask |
author_facet |
Knudsen, Steen Wilhelm Ebert, Rasmus Bach Hesselsøe, Martin Kuntke, Franziska Hassingboe, Jakob Mortensen, Peter Bondgaard Thomsen, Philip Francis Sigsgaard, Eva Egelyng Hansen, Brian Klitgaard Eg Nielsen, Einar Møller, Peter Rask |
author_sort |
Knudsen, Steen Wilhelm |
title |
Species-specific detection and quantification of environmental DNA from marine fishes in the Baltic Sea |
title_short |
Species-specific detection and quantification of environmental DNA from marine fishes in the Baltic Sea |
title_full |
Species-specific detection and quantification of environmental DNA from marine fishes in the Baltic Sea |
title_fullStr |
Species-specific detection and quantification of environmental DNA from marine fishes in the Baltic Sea |
title_full_unstemmed |
Species-specific detection and quantification of environmental DNA from marine fishes in the Baltic Sea |
title_sort |
species-specific detection and quantification of environmental dna from marine fishes in the baltic sea |
publishDate |
2019 |
url |
https://orbit.dtu.dk/en/publications/34f27ee8-83df-407a-b872-0b8f5782d483 https://doi.org/10.1016/j.jembe.2018.09.004 |
genre |
Anguilla anguilla atlantic cod European eel Gadus morhua |
genre_facet |
Anguilla anguilla atlantic cod European eel Gadus morhua |
op_source |
Knudsen , S W , Ebert , R B , Hesselsøe , M , Kuntke , F , Hassingboe , J , Mortensen , P B , Thomsen , P F , Sigsgaard , E E , Hansen , B K , Eg Nielsen , E & Møller , P R 2019 , ' Species-specific detection and quantification of environmental DNA from marine fishes in the Baltic Sea ' , Journal of Experimental Marine Biology and Ecology , vol. 510 , pp. 31-45 . https://doi.org/10.1016/j.jembe.2018.09.004 |
op_relation |
https://orbit.dtu.dk/en/publications/34f27ee8-83df-407a-b872-0b8f5782d483 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1016/j.jembe.2018.09.004 |
container_title |
Journal of Experimental Marine Biology and Ecology |
container_volume |
510 |
container_start_page |
31 |
op_container_end_page |
45 |
_version_ |
1802640957323083776 |