Morphomolecular alterations in the skin mucosa of Atlantic salmon ( Salmo salar ) after exposure to peracetic acid-based disinfectant
Peroxygen-based chemotherapeutants are commonly used in treating ectoparasitic infections in fish. A majority of relevant studies address the effects of these substances on the targeted causative agents, but little is known about their physiological impacts on the host organism. This study documente...
Published in: | Aquaculture Reports |
---|---|
Main Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2020
|
Subjects: | |
Online Access: | https://orbit.dtu.dk/en/publications/2a619455-697e-42cf-9a53-2596e98cbb1a https://doi.org/10.1016/j.aqrep.2020.100368 https://backend.orbit.dtu.dk/ws/files/213365772/1_s2.0_S2352513420301290_main.pdf |
id |
ftdtupubl:oai:pure.atira.dk:publications/2a619455-697e-42cf-9a53-2596e98cbb1a |
---|---|
record_format |
openpolar |
spelling |
ftdtupubl:oai:pure.atira.dk:publications/2a619455-697e-42cf-9a53-2596e98cbb1a 2024-05-19T07:37:41+00:00 Morphomolecular alterations in the skin mucosa of Atlantic salmon ( Salmo salar ) after exposure to peracetic acid-based disinfectant Lazado, Carlo Cabacang Haddeland, Sindre Timmerhaus, Gerrit Berg, Ragnhild Stenberg Merkin, Grigory Pittman, Karin Pedersen, Lars-Flemming 2020 application/pdf https://orbit.dtu.dk/en/publications/2a619455-697e-42cf-9a53-2596e98cbb1a https://doi.org/10.1016/j.aqrep.2020.100368 https://backend.orbit.dtu.dk/ws/files/213365772/1_s2.0_S2352513420301290_main.pdf eng eng https://orbit.dtu.dk/en/publications/2a619455-697e-42cf-9a53-2596e98cbb1a info:eu-repo/semantics/openAccess Lazado , C C , Haddeland , S , Timmerhaus , G , Berg , R S , Merkin , G , Pittman , K & Pedersen , L-F 2020 , ' Morphomolecular alterations in the skin mucosa of Atlantic salmon ( Salmo salar ) after exposure to peracetic acid-based disinfectant ' , Aquaculture Reports , vol. 17 , 100368 . https://doi.org/10.1016/j.aqrep.2020.100368 Aquaculture Disinfectant Mucous cells Skin health Peracetic acid Hydrogen peroxide article 2020 ftdtupubl https://doi.org/10.1016/j.aqrep.2020.100368 2024-04-24T00:49:01Z Peroxygen-based chemotherapeutants are commonly used in treating ectoparasitic infections in fish. A majority of relevant studies address the effects of these substances on the targeted causative agents, but little is known about their physiological impacts on the host organism. This study documented the changes in the skin of Atlantic salmon ( Salmo salar ) exposed to peracetic acid (PAA), a peroxygen disinfectant with potent oxidative properties. Fish were exposed to three therapeutic concentrations of PAA-based disinfectant: 0, 0.6, and 2.4 ppm. The initial exposure time was 5 min, and two weeks after, fish were re-exposed to the same doses for 30 min. Skin colour was not dramatically affected by the PAA treatments. No gross pathologies, lesions, or wounds were observed in the skin of sampled fish. Histological evaluation revealed that fish treated with PAA appeared to have rough epidermal surface compared with the 0 ppm group, especially after the re-exposure. Morphometrics of mucous cells did not markedly vary amongst the treatments, although the group treated with 2.4 ppm displayed a relatively larger mucous cells 2 weeks after the first exposure. Transcriptional analysis was conducted for key markers that were previously identified to be involved in the mucosal response to PAA, and the results revealed that proteolysis-related genes were modulated more remarkably during the first exposure than during re-exposure. These data revealed that therapeutic doses of PAA induced morphomolecular changes in the skin of salmon, although the magnitude of alteration was marginal. Article in Journal/Newspaper Atlantic salmon Salmo salar Technical University of Denmark: DTU Orbit Aquaculture Reports 17 100368 |
institution |
Open Polar |
collection |
Technical University of Denmark: DTU Orbit |
op_collection_id |
ftdtupubl |
language |
English |
topic |
Aquaculture Disinfectant Mucous cells Skin health Peracetic acid Hydrogen peroxide |
spellingShingle |
Aquaculture Disinfectant Mucous cells Skin health Peracetic acid Hydrogen peroxide Lazado, Carlo Cabacang Haddeland, Sindre Timmerhaus, Gerrit Berg, Ragnhild Stenberg Merkin, Grigory Pittman, Karin Pedersen, Lars-Flemming Morphomolecular alterations in the skin mucosa of Atlantic salmon ( Salmo salar ) after exposure to peracetic acid-based disinfectant |
topic_facet |
Aquaculture Disinfectant Mucous cells Skin health Peracetic acid Hydrogen peroxide |
description |
Peroxygen-based chemotherapeutants are commonly used in treating ectoparasitic infections in fish. A majority of relevant studies address the effects of these substances on the targeted causative agents, but little is known about their physiological impacts on the host organism. This study documented the changes in the skin of Atlantic salmon ( Salmo salar ) exposed to peracetic acid (PAA), a peroxygen disinfectant with potent oxidative properties. Fish were exposed to three therapeutic concentrations of PAA-based disinfectant: 0, 0.6, and 2.4 ppm. The initial exposure time was 5 min, and two weeks after, fish were re-exposed to the same doses for 30 min. Skin colour was not dramatically affected by the PAA treatments. No gross pathologies, lesions, or wounds were observed in the skin of sampled fish. Histological evaluation revealed that fish treated with PAA appeared to have rough epidermal surface compared with the 0 ppm group, especially after the re-exposure. Morphometrics of mucous cells did not markedly vary amongst the treatments, although the group treated with 2.4 ppm displayed a relatively larger mucous cells 2 weeks after the first exposure. Transcriptional analysis was conducted for key markers that were previously identified to be involved in the mucosal response to PAA, and the results revealed that proteolysis-related genes were modulated more remarkably during the first exposure than during re-exposure. These data revealed that therapeutic doses of PAA induced morphomolecular changes in the skin of salmon, although the magnitude of alteration was marginal. |
format |
Article in Journal/Newspaper |
author |
Lazado, Carlo Cabacang Haddeland, Sindre Timmerhaus, Gerrit Berg, Ragnhild Stenberg Merkin, Grigory Pittman, Karin Pedersen, Lars-Flemming |
author_facet |
Lazado, Carlo Cabacang Haddeland, Sindre Timmerhaus, Gerrit Berg, Ragnhild Stenberg Merkin, Grigory Pittman, Karin Pedersen, Lars-Flemming |
author_sort |
Lazado, Carlo Cabacang |
title |
Morphomolecular alterations in the skin mucosa of Atlantic salmon ( Salmo salar ) after exposure to peracetic acid-based disinfectant |
title_short |
Morphomolecular alterations in the skin mucosa of Atlantic salmon ( Salmo salar ) after exposure to peracetic acid-based disinfectant |
title_full |
Morphomolecular alterations in the skin mucosa of Atlantic salmon ( Salmo salar ) after exposure to peracetic acid-based disinfectant |
title_fullStr |
Morphomolecular alterations in the skin mucosa of Atlantic salmon ( Salmo salar ) after exposure to peracetic acid-based disinfectant |
title_full_unstemmed |
Morphomolecular alterations in the skin mucosa of Atlantic salmon ( Salmo salar ) after exposure to peracetic acid-based disinfectant |
title_sort |
morphomolecular alterations in the skin mucosa of atlantic salmon ( salmo salar ) after exposure to peracetic acid-based disinfectant |
publishDate |
2020 |
url |
https://orbit.dtu.dk/en/publications/2a619455-697e-42cf-9a53-2596e98cbb1a https://doi.org/10.1016/j.aqrep.2020.100368 https://backend.orbit.dtu.dk/ws/files/213365772/1_s2.0_S2352513420301290_main.pdf |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Lazado , C C , Haddeland , S , Timmerhaus , G , Berg , R S , Merkin , G , Pittman , K & Pedersen , L-F 2020 , ' Morphomolecular alterations in the skin mucosa of Atlantic salmon ( Salmo salar ) after exposure to peracetic acid-based disinfectant ' , Aquaculture Reports , vol. 17 , 100368 . https://doi.org/10.1016/j.aqrep.2020.100368 |
op_relation |
https://orbit.dtu.dk/en/publications/2a619455-697e-42cf-9a53-2596e98cbb1a |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1016/j.aqrep.2020.100368 |
container_title |
Aquaculture Reports |
container_volume |
17 |
container_start_page |
100368 |
_version_ |
1799477035014291456 |