Novel, Pseudomonas-derived Antifungal Lipopeptides From A Disease Suppressive Soil In Greenlandic Potato Fields
Potato cultivation in southwest Greenland omits the use of pesticides, relies on limited crop rotations, and despite the presence of plant pathogenic fungi in the soil, has not suffered from severe disease outbreaks. In this presentation, the bacterial strain Pseudomonas sp. In5 which significantly...
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ftdtupubl:oai:pure.atira.dk:publications/296acf5c-e6be-48d8-86ff-5a8db4946659 2023-12-24T10:17:09+01:00 Novel, Pseudomonas-derived Antifungal Lipopeptides From A Disease Suppressive Soil In Greenlandic Potato Fields Hennessy, Rosanna Catherine Michelsen, Charlotte F. Glaring, Mikkel A. Phippen, Christopher Nielsen, Kristian Fog Olsson, Stefan Stougaard, Peter 2015 https://orbit.dtu.dk/en/publications/296acf5c-e6be-48d8-86ff-5a8db4946659 eng eng https://orbit.dtu.dk/en/publications/296acf5c-e6be-48d8-86ff-5a8db4946659 info:eu-repo/semantics/closedAccess Hennessy , R C , Michelsen , C F , Glaring , M A , Phippen , C , Nielsen , K F , Olsson , S & Stougaard , P 2015 , Novel, Pseudomonas-derived Antifungal Lipopeptides From A Disease Suppressive Soil In Greenlandic Potato Fields . in The Danish Microbiological Society Annual Congress 2015 : Programme & Abstracts . Copenhagen , pp. 19-19 , The Danish Microbiological Society Annual Congress 2015 , Copenhagen , Denmark , 09/11/2015 . conferenceObject 2015 ftdtupubl 2023-11-30T00:00:46Z Potato cultivation in southwest Greenland omits the use of pesticides, relies on limited crop rotations, and despite the presence of plant pathogenic fungi in the soil, has not suffered from severe disease outbreaks. In this presentation, the bacterial strain Pseudomonas sp. In5 which significantly contributes to the suppressiveness of soil at Inneruulalik in southern Greenland will be explored. A combination of molecular genetics and genomics coupled with matrix-assisted laser desorption ionization – time of flight (MALDI-TOF) imaging mass spectrometry (IMS) identified a large genomic island encoding the two non-ribosomal peptides nunapeptin and nunamycin, which are key components of the antifungal activity of In5. Bacterial-fungal interaction studies uncovered a complex interaction whereby nunamycin appears most active against Rhizoctonia solani with no antimicrobial effect against the oomycete Pythium aphanidermatum . In constrast, nunapeptin is most potent against Pythium aphanidermatum in addition to Fusarium sp. To investigate the genetic regulation of both peptides, we have examined the diversity of LuxR-type regulators across the genomic island including upstream and downstream regions flanking the peptide biosynthetic genes. Functional analysis by knockout and complementation studies together with liquid chromatography – high resolution mass spectrometry (LC-HRMS) showed loss and gain of both antifungal activity and peptide synthesis. Current studies are aimed at unravelling further the complex regulation and mode of action of both peptides in order to develop effective microbial biocontrol agents (mBCAs). Conference Object Greenland greenlandic Technical University of Denmark: DTU Orbit Greenland |
institution |
Open Polar |
collection |
Technical University of Denmark: DTU Orbit |
op_collection_id |
ftdtupubl |
language |
English |
description |
Potato cultivation in southwest Greenland omits the use of pesticides, relies on limited crop rotations, and despite the presence of plant pathogenic fungi in the soil, has not suffered from severe disease outbreaks. In this presentation, the bacterial strain Pseudomonas sp. In5 which significantly contributes to the suppressiveness of soil at Inneruulalik in southern Greenland will be explored. A combination of molecular genetics and genomics coupled with matrix-assisted laser desorption ionization – time of flight (MALDI-TOF) imaging mass spectrometry (IMS) identified a large genomic island encoding the two non-ribosomal peptides nunapeptin and nunamycin, which are key components of the antifungal activity of In5. Bacterial-fungal interaction studies uncovered a complex interaction whereby nunamycin appears most active against Rhizoctonia solani with no antimicrobial effect against the oomycete Pythium aphanidermatum . In constrast, nunapeptin is most potent against Pythium aphanidermatum in addition to Fusarium sp. To investigate the genetic regulation of both peptides, we have examined the diversity of LuxR-type regulators across the genomic island including upstream and downstream regions flanking the peptide biosynthetic genes. Functional analysis by knockout and complementation studies together with liquid chromatography – high resolution mass spectrometry (LC-HRMS) showed loss and gain of both antifungal activity and peptide synthesis. Current studies are aimed at unravelling further the complex regulation and mode of action of both peptides in order to develop effective microbial biocontrol agents (mBCAs). |
format |
Conference Object |
author |
Hennessy, Rosanna Catherine Michelsen, Charlotte F. Glaring, Mikkel A. Phippen, Christopher Nielsen, Kristian Fog Olsson, Stefan Stougaard, Peter |
spellingShingle |
Hennessy, Rosanna Catherine Michelsen, Charlotte F. Glaring, Mikkel A. Phippen, Christopher Nielsen, Kristian Fog Olsson, Stefan Stougaard, Peter Novel, Pseudomonas-derived Antifungal Lipopeptides From A Disease Suppressive Soil In Greenlandic Potato Fields |
author_facet |
Hennessy, Rosanna Catherine Michelsen, Charlotte F. Glaring, Mikkel A. Phippen, Christopher Nielsen, Kristian Fog Olsson, Stefan Stougaard, Peter |
author_sort |
Hennessy, Rosanna Catherine |
title |
Novel, Pseudomonas-derived Antifungal Lipopeptides From A Disease Suppressive Soil In Greenlandic Potato Fields |
title_short |
Novel, Pseudomonas-derived Antifungal Lipopeptides From A Disease Suppressive Soil In Greenlandic Potato Fields |
title_full |
Novel, Pseudomonas-derived Antifungal Lipopeptides From A Disease Suppressive Soil In Greenlandic Potato Fields |
title_fullStr |
Novel, Pseudomonas-derived Antifungal Lipopeptides From A Disease Suppressive Soil In Greenlandic Potato Fields |
title_full_unstemmed |
Novel, Pseudomonas-derived Antifungal Lipopeptides From A Disease Suppressive Soil In Greenlandic Potato Fields |
title_sort |
novel, pseudomonas-derived antifungal lipopeptides from a disease suppressive soil in greenlandic potato fields |
publishDate |
2015 |
url |
https://orbit.dtu.dk/en/publications/296acf5c-e6be-48d8-86ff-5a8db4946659 |
geographic |
Greenland |
geographic_facet |
Greenland |
genre |
Greenland greenlandic |
genre_facet |
Greenland greenlandic |
op_source |
Hennessy , R C , Michelsen , C F , Glaring , M A , Phippen , C , Nielsen , K F , Olsson , S & Stougaard , P 2015 , Novel, Pseudomonas-derived Antifungal Lipopeptides From A Disease Suppressive Soil In Greenlandic Potato Fields . in The Danish Microbiological Society Annual Congress 2015 : Programme & Abstracts . Copenhagen , pp. 19-19 , The Danish Microbiological Society Annual Congress 2015 , Copenhagen , Denmark , 09/11/2015 . |
op_relation |
https://orbit.dtu.dk/en/publications/296acf5c-e6be-48d8-86ff-5a8db4946659 |
op_rights |
info:eu-repo/semantics/closedAccess |
_version_ |
1786205083619295232 |