Fate of antibiotic resistance genes in two Arctic tundra wetlands impacted by municipal wastewater
In the Canadian Arctic, it is common practice to discharge municipal wastewater into tundra wetlands. Antibiotic resistant bacteria and the antibiotic resistance genes (ARGs) they contain can be present in municipal wastewater and there is a scarcity of knowledge on ARGs in wastewater in Arctic envi...
Published in: | Science of The Total Environment |
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2018
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Online Access: | https://orbit.dtu.dk/en/publications/27f7d7d3-1a19-4776-98c2-19151b9bbb58 https://doi.org/10.1016/j.scitotenv.2018.06.083 https://backend.orbit.dtu.dk/ws/files/153263487/Post_print_Hayward_et_al_2018_Arg_in_NU_wetlands_STOTEN_07_05_2018.pdf |
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ftdtupubl:oai:pure.atira.dk:publications/27f7d7d3-1a19-4776-98c2-19151b9bbb58 2023-11-12T04:09:52+01:00 Fate of antibiotic resistance genes in two Arctic tundra wetlands impacted by municipal wastewater Hayward, Jennifer L. Jackson, Amy J. Yost, Christopher K. Hansen, Lisbeth Truelstrup Jamieson, Rob C. 2018 application/pdf https://orbit.dtu.dk/en/publications/27f7d7d3-1a19-4776-98c2-19151b9bbb58 https://doi.org/10.1016/j.scitotenv.2018.06.083 https://backend.orbit.dtu.dk/ws/files/153263487/Post_print_Hayward_et_al_2018_Arg_in_NU_wetlands_STOTEN_07_05_2018.pdf eng eng info:eu-repo/semantics/openAccess Hayward , J L , Jackson , A J , Yost , C K , Hansen , L T & Jamieson , R C 2018 , ' Fate of antibiotic resistance genes in two Arctic tundra wetlands impacted by municipal wastewater ' , Science of the Total Environment , vol. 642 , pp. 1415-1428 . https://doi.org/10.1016/j.scitotenv.2018.06.083 ARG Tundra wetland hydrology qPCR Cold climate Arctic Antimicrobial resistance article 2018 ftdtupubl https://doi.org/10.1016/j.scitotenv.2018.06.083 2023-10-18T22:58:15Z In the Canadian Arctic, it is common practice to discharge municipal wastewater into tundra wetlands. Antibiotic resistant bacteria and the antibiotic resistance genes (ARGs) they contain can be present in municipal wastewater and there is a scarcity of knowledge on ARGs in wastewater in Arctic environments. This study was initiated on the fate of ARGs in tundra wetland ecosystems impacted by anthropogenic wastewater sources in Arctic communities. In the summer season of 2016, two wetlands were studied in the Inuit communities of Sanikiluaq and Naujaat in Nunavut, Canada. Genomic DNA was extracted from both soil and water during the spring freshet and late summer in the wetlands, and a suite of nine clinically relevant ARGs ( sul1, sul2, mecA, vanA, qnrS, ermB, tetO, bla TEM , bla CTX-M ), and an integron gene ( int1 ) were analyzed using quantitative polymerase chain reaction (qPCR). Hydrological and water quality measurements were conducted in conjunction with the microbiological sampling. Gene targets were consistently present in the wastewater, and throughout both wetlands, except for vanA and mecA . Concentrations of ARGs were greater during the spring freshet, due to short hydraulic retention times (<2 days), which coincided with decreased treatment performance. The environmental resistome in un-impacted wetlands had above detection limit concentrations of int1, sul1, sul2, bla CTX-M in water in Naujaat, and sul1, qnrS and tetO in soil in Sanikiluaq. First-order rate constants were widely variable and specific to the gene target. ARGs were present in concentrations elevated above baseline reference sites in tundra wetlands influenced by municipal wastewater, and hydrological conditions had a large impact on their spatial distribution and levels. Article in Journal/Newspaper Arctic Arctic inuit Naujaat Nunavut Sanikiluaq Tundra Technical University of Denmark: DTU Orbit Arctic Canada Naujaat ENVELOPE(-86.244,-86.244,66.529,66.529) Nunavut Sanikiluaq ENVELOPE(-79.226,-79.226,56.541,56.541) Science of The Total Environment 642 1415 1428 |
institution |
Open Polar |
collection |
Technical University of Denmark: DTU Orbit |
op_collection_id |
ftdtupubl |
language |
English |
topic |
ARG Tundra wetland hydrology qPCR Cold climate Arctic Antimicrobial resistance |
spellingShingle |
ARG Tundra wetland hydrology qPCR Cold climate Arctic Antimicrobial resistance Hayward, Jennifer L. Jackson, Amy J. Yost, Christopher K. Hansen, Lisbeth Truelstrup Jamieson, Rob C. Fate of antibiotic resistance genes in two Arctic tundra wetlands impacted by municipal wastewater |
topic_facet |
ARG Tundra wetland hydrology qPCR Cold climate Arctic Antimicrobial resistance |
description |
In the Canadian Arctic, it is common practice to discharge municipal wastewater into tundra wetlands. Antibiotic resistant bacteria and the antibiotic resistance genes (ARGs) they contain can be present in municipal wastewater and there is a scarcity of knowledge on ARGs in wastewater in Arctic environments. This study was initiated on the fate of ARGs in tundra wetland ecosystems impacted by anthropogenic wastewater sources in Arctic communities. In the summer season of 2016, two wetlands were studied in the Inuit communities of Sanikiluaq and Naujaat in Nunavut, Canada. Genomic DNA was extracted from both soil and water during the spring freshet and late summer in the wetlands, and a suite of nine clinically relevant ARGs ( sul1, sul2, mecA, vanA, qnrS, ermB, tetO, bla TEM , bla CTX-M ), and an integron gene ( int1 ) were analyzed using quantitative polymerase chain reaction (qPCR). Hydrological and water quality measurements were conducted in conjunction with the microbiological sampling. Gene targets were consistently present in the wastewater, and throughout both wetlands, except for vanA and mecA . Concentrations of ARGs were greater during the spring freshet, due to short hydraulic retention times (<2 days), which coincided with decreased treatment performance. The environmental resistome in un-impacted wetlands had above detection limit concentrations of int1, sul1, sul2, bla CTX-M in water in Naujaat, and sul1, qnrS and tetO in soil in Sanikiluaq. First-order rate constants were widely variable and specific to the gene target. ARGs were present in concentrations elevated above baseline reference sites in tundra wetlands influenced by municipal wastewater, and hydrological conditions had a large impact on their spatial distribution and levels. |
format |
Article in Journal/Newspaper |
author |
Hayward, Jennifer L. Jackson, Amy J. Yost, Christopher K. Hansen, Lisbeth Truelstrup Jamieson, Rob C. |
author_facet |
Hayward, Jennifer L. Jackson, Amy J. Yost, Christopher K. Hansen, Lisbeth Truelstrup Jamieson, Rob C. |
author_sort |
Hayward, Jennifer L. |
title |
Fate of antibiotic resistance genes in two Arctic tundra wetlands impacted by municipal wastewater |
title_short |
Fate of antibiotic resistance genes in two Arctic tundra wetlands impacted by municipal wastewater |
title_full |
Fate of antibiotic resistance genes in two Arctic tundra wetlands impacted by municipal wastewater |
title_fullStr |
Fate of antibiotic resistance genes in two Arctic tundra wetlands impacted by municipal wastewater |
title_full_unstemmed |
Fate of antibiotic resistance genes in two Arctic tundra wetlands impacted by municipal wastewater |
title_sort |
fate of antibiotic resistance genes in two arctic tundra wetlands impacted by municipal wastewater |
publishDate |
2018 |
url |
https://orbit.dtu.dk/en/publications/27f7d7d3-1a19-4776-98c2-19151b9bbb58 https://doi.org/10.1016/j.scitotenv.2018.06.083 https://backend.orbit.dtu.dk/ws/files/153263487/Post_print_Hayward_et_al_2018_Arg_in_NU_wetlands_STOTEN_07_05_2018.pdf |
long_lat |
ENVELOPE(-86.244,-86.244,66.529,66.529) ENVELOPE(-79.226,-79.226,56.541,56.541) |
geographic |
Arctic Canada Naujaat Nunavut Sanikiluaq |
geographic_facet |
Arctic Canada Naujaat Nunavut Sanikiluaq |
genre |
Arctic Arctic inuit Naujaat Nunavut Sanikiluaq Tundra |
genre_facet |
Arctic Arctic inuit Naujaat Nunavut Sanikiluaq Tundra |
op_source |
Hayward , J L , Jackson , A J , Yost , C K , Hansen , L T & Jamieson , R C 2018 , ' Fate of antibiotic resistance genes in two Arctic tundra wetlands impacted by municipal wastewater ' , Science of the Total Environment , vol. 642 , pp. 1415-1428 . https://doi.org/10.1016/j.scitotenv.2018.06.083 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1016/j.scitotenv.2018.06.083 |
container_title |
Science of The Total Environment |
container_volume |
642 |
container_start_page |
1415 |
op_container_end_page |
1428 |
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1782329634987180032 |