First Assessment of Prebiotics, Probiotics, and Synbiotics Affecting Survival, Growth, and Gene Expression of European Eel ( Anguilla anguilla ) Larvae
European eel, Anguilla anguilla , larval culture faces a bottleneck during the transition to exogenous feeding. To stimulate gut-priming, in the present study, prebiotics (AgriMOS, mannan-oligosaccharides, and β-(1,3 and 1,6)-poly-D-glucose), probiotics (Bactocell, Pediococcus acidilactici ), and sy...
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2023
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ftdtupubl:oai:pure.atira.dk:publications/27bca68c-70da-420d-a4d7-700e7f90d6ce 2024-09-15T17:39:38+00:00 First Assessment of Prebiotics, Probiotics, and Synbiotics Affecting Survival, Growth, and Gene Expression of European Eel ( Anguilla anguilla ) Larvae N. Politis, Sebastian Benini, Elisa J. Miest, Joanna Engrola, Sofia R. Sørensen, Sune Syropoulou, Elisavet A. E. Butts, Ian Tomkiewicz, Jonna 2023 application/pdf https://orbit.dtu.dk/en/publications/27bca68c-70da-420d-a4d7-700e7f90d6ce https://doi.org/10.1155/2023/1260967 https://backend.orbit.dtu.dk/ws/files/314081786/1260967.pdf eng eng https://orbit.dtu.dk/en/publications/27bca68c-70da-420d-a4d7-700e7f90d6ce info:eu-repo/semantics/openAccess N. Politis , S , Benini , E , J. Miest , J , Engrola , S , R. Sørensen , S , Syropoulou , E , A. E. Butts , I & Tomkiewicz , J 2023 , ' First Assessment of Prebiotics, Probiotics, and Synbiotics Affecting Survival, Growth, and Gene Expression of European Eel ( Anguilla anguilla ) Larvae ' , Aquaculture Research , vol. 2023 , 1260967 . https://doi.org/10.1155/2023/1260967 article 2023 ftdtupubl https://doi.org/10.1155/2023/1260967 2024-08-13T00:03:06Z European eel, Anguilla anguilla , larval culture faces a bottleneck during the transition to exogenous feeding. To stimulate gut-priming, in the present study, prebiotics (AgriMOS, mannan-oligosaccharides, and β-(1,3 and 1,6)-poly-D-glucose), probiotics (Bactocell, Pediococcus acidilactici ), and synbiotics (AgriMOS + Bactocell) were administered to European eel larvae during the endogenous prefeeding stage. Eel larvae were reared in 2 L incubators with an initial stocking density of ∼200 larvae/L. Each treatment (pre-, pro-, synbiotics, and control), represented by 3 replicated incubators, was connected to a separate recirculating aquaculture system. The gut-priming agents were introduced directly into the rearing water. Results revealed increased mortality when larvae were introduced to synbiotics and impaired growth in connection to probiotics and synbiotics. Larvae receiving prebiotics showed similar survival and growth to larvae reared without gut-priming agents. The immune gene expression revealed a lag phase between maternally inherited protection ( c3, igm , and il10 ) and the gradual buildup of the larvae’s own immune system ( il1β , irf7 ). The lack of treatment-related immune ( c3, igm, il10, il1β, and irf7 ) and stress/repair ( hsp70, hsp90 ) responses revealed an immature immuno-readiness. Digestion ( try, ctra, ctrb, tgl , and amyl ), food intake ( cck ), and appetite ( ghrl )-related genes were expressed at basal levels already on 4 days post-hatch, which combined with phenotypic plasticity of the appetite-regulating ghrelin ( ghrl ), indicated a prospective adaptive capability towards earlier maturation of the larval digestive capacity. Overall, we contemplate that the application of gut-priming agents in water has merit; however, as no beneficial effect was observed, we conclude that the regimen applied is not recommendable in the present form and needs to be customized for future eel larval culture. As such, water management strategies and rearing options need to be further explored to ... Article in Journal/Newspaper Anguilla anguilla Technical University of Denmark: DTU Orbit Aquaculture Research 2023 1 13 |
institution |
Open Polar |
collection |
Technical University of Denmark: DTU Orbit |
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ftdtupubl |
language |
English |
description |
European eel, Anguilla anguilla , larval culture faces a bottleneck during the transition to exogenous feeding. To stimulate gut-priming, in the present study, prebiotics (AgriMOS, mannan-oligosaccharides, and β-(1,3 and 1,6)-poly-D-glucose), probiotics (Bactocell, Pediococcus acidilactici ), and synbiotics (AgriMOS + Bactocell) were administered to European eel larvae during the endogenous prefeeding stage. Eel larvae were reared in 2 L incubators with an initial stocking density of ∼200 larvae/L. Each treatment (pre-, pro-, synbiotics, and control), represented by 3 replicated incubators, was connected to a separate recirculating aquaculture system. The gut-priming agents were introduced directly into the rearing water. Results revealed increased mortality when larvae were introduced to synbiotics and impaired growth in connection to probiotics and synbiotics. Larvae receiving prebiotics showed similar survival and growth to larvae reared without gut-priming agents. The immune gene expression revealed a lag phase between maternally inherited protection ( c3, igm , and il10 ) and the gradual buildup of the larvae’s own immune system ( il1β , irf7 ). The lack of treatment-related immune ( c3, igm, il10, il1β, and irf7 ) and stress/repair ( hsp70, hsp90 ) responses revealed an immature immuno-readiness. Digestion ( try, ctra, ctrb, tgl , and amyl ), food intake ( cck ), and appetite ( ghrl )-related genes were expressed at basal levels already on 4 days post-hatch, which combined with phenotypic plasticity of the appetite-regulating ghrelin ( ghrl ), indicated a prospective adaptive capability towards earlier maturation of the larval digestive capacity. Overall, we contemplate that the application of gut-priming agents in water has merit; however, as no beneficial effect was observed, we conclude that the regimen applied is not recommendable in the present form and needs to be customized for future eel larval culture. As such, water management strategies and rearing options need to be further explored to ... |
format |
Article in Journal/Newspaper |
author |
N. Politis, Sebastian Benini, Elisa J. Miest, Joanna Engrola, Sofia R. Sørensen, Sune Syropoulou, Elisavet A. E. Butts, Ian Tomkiewicz, Jonna |
spellingShingle |
N. Politis, Sebastian Benini, Elisa J. Miest, Joanna Engrola, Sofia R. Sørensen, Sune Syropoulou, Elisavet A. E. Butts, Ian Tomkiewicz, Jonna First Assessment of Prebiotics, Probiotics, and Synbiotics Affecting Survival, Growth, and Gene Expression of European Eel ( Anguilla anguilla ) Larvae |
author_facet |
N. Politis, Sebastian Benini, Elisa J. Miest, Joanna Engrola, Sofia R. Sørensen, Sune Syropoulou, Elisavet A. E. Butts, Ian Tomkiewicz, Jonna |
author_sort |
N. Politis, Sebastian |
title |
First Assessment of Prebiotics, Probiotics, and Synbiotics Affecting Survival, Growth, and Gene Expression of European Eel ( Anguilla anguilla ) Larvae |
title_short |
First Assessment of Prebiotics, Probiotics, and Synbiotics Affecting Survival, Growth, and Gene Expression of European Eel ( Anguilla anguilla ) Larvae |
title_full |
First Assessment of Prebiotics, Probiotics, and Synbiotics Affecting Survival, Growth, and Gene Expression of European Eel ( Anguilla anguilla ) Larvae |
title_fullStr |
First Assessment of Prebiotics, Probiotics, and Synbiotics Affecting Survival, Growth, and Gene Expression of European Eel ( Anguilla anguilla ) Larvae |
title_full_unstemmed |
First Assessment of Prebiotics, Probiotics, and Synbiotics Affecting Survival, Growth, and Gene Expression of European Eel ( Anguilla anguilla ) Larvae |
title_sort |
first assessment of prebiotics, probiotics, and synbiotics affecting survival, growth, and gene expression of european eel ( anguilla anguilla ) larvae |
publishDate |
2023 |
url |
https://orbit.dtu.dk/en/publications/27bca68c-70da-420d-a4d7-700e7f90d6ce https://doi.org/10.1155/2023/1260967 https://backend.orbit.dtu.dk/ws/files/314081786/1260967.pdf |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_source |
N. Politis , S , Benini , E , J. Miest , J , Engrola , S , R. Sørensen , S , Syropoulou , E , A. E. Butts , I & Tomkiewicz , J 2023 , ' First Assessment of Prebiotics, Probiotics, and Synbiotics Affecting Survival, Growth, and Gene Expression of European Eel ( Anguilla anguilla ) Larvae ' , Aquaculture Research , vol. 2023 , 1260967 . https://doi.org/10.1155/2023/1260967 |
op_relation |
https://orbit.dtu.dk/en/publications/27bca68c-70da-420d-a4d7-700e7f90d6ce |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1155/2023/1260967 |
container_title |
Aquaculture Research |
container_volume |
2023 |
container_start_page |
1 |
op_container_end_page |
13 |
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1810481398098690048 |