Infection experiments with novel Piscine orthoreovirus from rainbow trout (Oncorhynchus mykiss) in salmonids

A new disease in farmed rainbow trout ( Onchorhyncus mykiss ) was described in Norway in 2013. The disease mainly affected the heart and resembled heart and skeletal muscle inflammation (HSMI) in Atlantic salmon ( Salmo salar L. ). HSMI is associated with Piscine orthoreovirus (PRV), and a search fo...

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Published in:PLOS ONE
Main Authors: Hauge, Helena, Vendramin, Niccolò, Taksdal, Torunn, Olsen, Anne Berit, Wessel, Øystein, Mikkelsen, Susie Sommer, Alencar, Anna Luiza Farias, Olesen, Niels Jørgen, Dahle, Maria Krudtaa
Format: Article in Journal/Newspaper
Language:English
Published: 2017
Subjects:
Online Access:https://orbit.dtu.dk/en/publications/2491c5e3-9d48-4f25-971d-1992f3ac73e2
https://doi.org/10.1371/journal.pone.0180293
https://backend.orbit.dtu.dk/ws/files/133891761/journal.pone.0180293.pdf
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spelling ftdtupubl:oai:pure.atira.dk:publications/2491c5e3-9d48-4f25-971d-1992f3ac73e2 2024-09-15T17:56:06+00:00 Infection experiments with novel Piscine orthoreovirus from rainbow trout (Oncorhynchus mykiss) in salmonids Hauge, Helena Vendramin, Niccolò Taksdal, Torunn Olsen, Anne Berit Wessel, Øystein Mikkelsen, Susie Sommer Alencar, Anna Luiza Farias Olesen, Niels Jørgen Dahle, Maria Krudtaa 2017 application/pdf https://orbit.dtu.dk/en/publications/2491c5e3-9d48-4f25-971d-1992f3ac73e2 https://doi.org/10.1371/journal.pone.0180293 https://backend.orbit.dtu.dk/ws/files/133891761/journal.pone.0180293.pdf eng eng https://orbit.dtu.dk/en/publications/2491c5e3-9d48-4f25-971d-1992f3ac73e2 info:eu-repo/semantics/openAccess Hauge , H , Vendramin , N , Taksdal , T , Olsen , A B , Wessel , Ø , Mikkelsen , S S , Alencar , A L F , Olesen , N J & Dahle , M K 2017 , ' Infection experiments with novel Piscine orthoreovirus from rainbow trout (Oncorhynchus mykiss) in salmonids ' , P L o S One , vol. 12 , no. 7 , e0180293 . https://doi.org/10.1371/journal.pone.0180293 article 2017 ftdtupubl https://doi.org/10.1371/journal.pone.0180293 2024-08-05T23:48:29Z A new disease in farmed rainbow trout ( Onchorhyncus mykiss ) was described in Norway in 2013. The disease mainly affected the heart and resembled heart and skeletal muscle inflammation (HSMI) in Atlantic salmon ( Salmo salar L. ). HSMI is associated with Piscine orthoreovirus (PRV), and a search for a similar virus in the diseased rainbow trout led to detection of a sequence with 85% similarity to PRV. This finding called for a targeted effort to assess the risk the new PRV-variant pose on farmed rainbow trout and Atlantic salmon by studying infection and disease pathogenesis, aiming to provide more diagnostic knowledge. Based on the genetic relationship to PRV, the novel virus is referred to as PRV- Oncorhynchus mykiss (PRV- Om ) in contrast to PRV- Salmo salar (PRV- Ss ). In experimental trials, intraperitoneally injected PRV- Om was shown to replicate in blood in both salmonid species, but more effectively in rainbow trout. In rainbow trout, the virus levels peaked in blood and heart of cohabitants 6 weeks post challenge, along with increased expression of antiviral genes (Mx and viperin) in the spleen, with 80-100% of the cohabitants infected. Heart inflammation was diagnosed in all cohabitants examined 8 weeks post challenge. In contrast, less than 50% of the Atlantic salmon cohabitants were infected between 8 and 16 weeks post challenge and the antiviral response in these fish was very low. From 12 weeks post challenge and onwards, mild focal myocarditis was demonstrated in a few virus-positive salmon. In conclusion, PRV- Om infects both salmonid species, but faster transmission, more notable antiviral response and more prominent heart pathology were observed in rainbow trout. Article in Journal/Newspaper Atlantic salmon Salmo salar Technical University of Denmark: DTU Orbit PLOS ONE 12 7 e0180293
institution Open Polar
collection Technical University of Denmark: DTU Orbit
op_collection_id ftdtupubl
language English
description A new disease in farmed rainbow trout ( Onchorhyncus mykiss ) was described in Norway in 2013. The disease mainly affected the heart and resembled heart and skeletal muscle inflammation (HSMI) in Atlantic salmon ( Salmo salar L. ). HSMI is associated with Piscine orthoreovirus (PRV), and a search for a similar virus in the diseased rainbow trout led to detection of a sequence with 85% similarity to PRV. This finding called for a targeted effort to assess the risk the new PRV-variant pose on farmed rainbow trout and Atlantic salmon by studying infection and disease pathogenesis, aiming to provide more diagnostic knowledge. Based on the genetic relationship to PRV, the novel virus is referred to as PRV- Oncorhynchus mykiss (PRV- Om ) in contrast to PRV- Salmo salar (PRV- Ss ). In experimental trials, intraperitoneally injected PRV- Om was shown to replicate in blood in both salmonid species, but more effectively in rainbow trout. In rainbow trout, the virus levels peaked in blood and heart of cohabitants 6 weeks post challenge, along with increased expression of antiviral genes (Mx and viperin) in the spleen, with 80-100% of the cohabitants infected. Heart inflammation was diagnosed in all cohabitants examined 8 weeks post challenge. In contrast, less than 50% of the Atlantic salmon cohabitants were infected between 8 and 16 weeks post challenge and the antiviral response in these fish was very low. From 12 weeks post challenge and onwards, mild focal myocarditis was demonstrated in a few virus-positive salmon. In conclusion, PRV- Om infects both salmonid species, but faster transmission, more notable antiviral response and more prominent heart pathology were observed in rainbow trout.
format Article in Journal/Newspaper
author Hauge, Helena
Vendramin, Niccolò
Taksdal, Torunn
Olsen, Anne Berit
Wessel, Øystein
Mikkelsen, Susie Sommer
Alencar, Anna Luiza Farias
Olesen, Niels Jørgen
Dahle, Maria Krudtaa
spellingShingle Hauge, Helena
Vendramin, Niccolò
Taksdal, Torunn
Olsen, Anne Berit
Wessel, Øystein
Mikkelsen, Susie Sommer
Alencar, Anna Luiza Farias
Olesen, Niels Jørgen
Dahle, Maria Krudtaa
Infection experiments with novel Piscine orthoreovirus from rainbow trout (Oncorhynchus mykiss) in salmonids
author_facet Hauge, Helena
Vendramin, Niccolò
Taksdal, Torunn
Olsen, Anne Berit
Wessel, Øystein
Mikkelsen, Susie Sommer
Alencar, Anna Luiza Farias
Olesen, Niels Jørgen
Dahle, Maria Krudtaa
author_sort Hauge, Helena
title Infection experiments with novel Piscine orthoreovirus from rainbow trout (Oncorhynchus mykiss) in salmonids
title_short Infection experiments with novel Piscine orthoreovirus from rainbow trout (Oncorhynchus mykiss) in salmonids
title_full Infection experiments with novel Piscine orthoreovirus from rainbow trout (Oncorhynchus mykiss) in salmonids
title_fullStr Infection experiments with novel Piscine orthoreovirus from rainbow trout (Oncorhynchus mykiss) in salmonids
title_full_unstemmed Infection experiments with novel Piscine orthoreovirus from rainbow trout (Oncorhynchus mykiss) in salmonids
title_sort infection experiments with novel piscine orthoreovirus from rainbow trout (oncorhynchus mykiss) in salmonids
publishDate 2017
url https://orbit.dtu.dk/en/publications/2491c5e3-9d48-4f25-971d-1992f3ac73e2
https://doi.org/10.1371/journal.pone.0180293
https://backend.orbit.dtu.dk/ws/files/133891761/journal.pone.0180293.pdf
genre Atlantic salmon
Salmo salar
genre_facet Atlantic salmon
Salmo salar
op_source Hauge , H , Vendramin , N , Taksdal , T , Olsen , A B , Wessel , Ø , Mikkelsen , S S , Alencar , A L F , Olesen , N J & Dahle , M K 2017 , ' Infection experiments with novel Piscine orthoreovirus from rainbow trout (Oncorhynchus mykiss) in salmonids ' , P L o S One , vol. 12 , no. 7 , e0180293 . https://doi.org/10.1371/journal.pone.0180293
op_relation https://orbit.dtu.dk/en/publications/2491c5e3-9d48-4f25-971d-1992f3ac73e2
op_rights info:eu-repo/semantics/openAccess
op_doi https://doi.org/10.1371/journal.pone.0180293
container_title PLOS ONE
container_volume 12
container_issue 7
container_start_page e0180293
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