Isolation and characterization of Caldicellulosiruptor lactoaceticus sp. nov., an extremely thermophilic, cellulolytic, anaerobic bacterium
An anaerobic, extremely thermophilic, cellulolytic, non-spore-forming bacterium, strain 6A, was isolated from an alkaline hot spring in Hverageroi, Iceland. The bacterium was non-motile, rod-shaped (1.5-3.5 x 0.7 mu m) and occurred singly, in pairs or in chains and stained gram-negative. The growth...
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ftdtupubl:oai:pure.atira.dk:publications/17a5b596-4bb0-4c35-9721-9d99a18e6ea1 2023-08-27T04:10:11+02:00 Isolation and characterization of Caldicellulosiruptor lactoaceticus sp. nov., an extremely thermophilic, cellulolytic, anaerobic bacterium Mladenovska, Zuzana Mathrani, Indra M. Ahring, Birgitte Kiær 1995 https://orbit.dtu.dk/en/publications/17a5b596-4bb0-4c35-9721-9d99a18e6ea1 https://doi.org/10.1007/BF00305357 eng eng info:eu-repo/semantics/restrictedAccess Mladenovska , Z , Mathrani , I M & Ahring , B K 1995 , ' Isolation and characterization of Caldicellulosiruptor lactoaceticus sp. nov., an extremely thermophilic, cellulolytic, anaerobic bacterium ' , Archives of Microbiology , vol. 163 , no. 3 , pp. 223-230 . https://doi.org/10.1007/BF00305357 article 1995 ftdtupubl https://doi.org/10.1007/BF00305357 2023-08-02T22:55:21Z An anaerobic, extremely thermophilic, cellulolytic, non-spore-forming bacterium, strain 6A, was isolated from an alkaline hot spring in Hverageroi, Iceland. The bacterium was non-motile, rod-shaped (1.5-3.5 x 0.7 mu m) and occurred singly, in pairs or in chains and stained gram-negative. The growth temperature was between 50 and 78 degrees C with a temperature optimum near 68 degrees C. Growth occurred between pH 5.8 and 8.2 with an optimum mum near 7.0. The bacterium fermented microcrystalline cellulose (Avicel) and produced lactate, acetate and H-2 as the major fermentation products, and CO2 and ethanol occurred as minor fermentation products. Only a restricted number of carbon sources (cellulose, xylan, starch, pectin, cellobiose, xylose, maltose and lactose) were used as substrates. During growth on Avicel, the bacterium produced free cellulases with carboxymethylcellulase and avicelase activity. The G + C content of the cellular DNA of strain 6A was 35.2 +/- 0.8 mol%. Complete 16S rDNA sequence analysis showed that strain 6A was phylogenetically related to Caldicellulosiruptor saccharolyticus. It is proposed that the isolated bacterium be named Caldicellulosiruptor lactoaceticus sp. nov. Article in Journal/Newspaper Iceland Technical University of Denmark: DTU Orbit Archives of Microbiology 163 3 223 230 |
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Open Polar |
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Technical University of Denmark: DTU Orbit |
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ftdtupubl |
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English |
description |
An anaerobic, extremely thermophilic, cellulolytic, non-spore-forming bacterium, strain 6A, was isolated from an alkaline hot spring in Hverageroi, Iceland. The bacterium was non-motile, rod-shaped (1.5-3.5 x 0.7 mu m) and occurred singly, in pairs or in chains and stained gram-negative. The growth temperature was between 50 and 78 degrees C with a temperature optimum near 68 degrees C. Growth occurred between pH 5.8 and 8.2 with an optimum mum near 7.0. The bacterium fermented microcrystalline cellulose (Avicel) and produced lactate, acetate and H-2 as the major fermentation products, and CO2 and ethanol occurred as minor fermentation products. Only a restricted number of carbon sources (cellulose, xylan, starch, pectin, cellobiose, xylose, maltose and lactose) were used as substrates. During growth on Avicel, the bacterium produced free cellulases with carboxymethylcellulase and avicelase activity. The G + C content of the cellular DNA of strain 6A was 35.2 +/- 0.8 mol%. Complete 16S rDNA sequence analysis showed that strain 6A was phylogenetically related to Caldicellulosiruptor saccharolyticus. It is proposed that the isolated bacterium be named Caldicellulosiruptor lactoaceticus sp. nov. |
format |
Article in Journal/Newspaper |
author |
Mladenovska, Zuzana Mathrani, Indra M. Ahring, Birgitte Kiær |
spellingShingle |
Mladenovska, Zuzana Mathrani, Indra M. Ahring, Birgitte Kiær Isolation and characterization of Caldicellulosiruptor lactoaceticus sp. nov., an extremely thermophilic, cellulolytic, anaerobic bacterium |
author_facet |
Mladenovska, Zuzana Mathrani, Indra M. Ahring, Birgitte Kiær |
author_sort |
Mladenovska, Zuzana |
title |
Isolation and characterization of Caldicellulosiruptor lactoaceticus sp. nov., an extremely thermophilic, cellulolytic, anaerobic bacterium |
title_short |
Isolation and characterization of Caldicellulosiruptor lactoaceticus sp. nov., an extremely thermophilic, cellulolytic, anaerobic bacterium |
title_full |
Isolation and characterization of Caldicellulosiruptor lactoaceticus sp. nov., an extremely thermophilic, cellulolytic, anaerobic bacterium |
title_fullStr |
Isolation and characterization of Caldicellulosiruptor lactoaceticus sp. nov., an extremely thermophilic, cellulolytic, anaerobic bacterium |
title_full_unstemmed |
Isolation and characterization of Caldicellulosiruptor lactoaceticus sp. nov., an extremely thermophilic, cellulolytic, anaerobic bacterium |
title_sort |
isolation and characterization of caldicellulosiruptor lactoaceticus sp. nov., an extremely thermophilic, cellulolytic, anaerobic bacterium |
publishDate |
1995 |
url |
https://orbit.dtu.dk/en/publications/17a5b596-4bb0-4c35-9721-9d99a18e6ea1 https://doi.org/10.1007/BF00305357 |
genre |
Iceland |
genre_facet |
Iceland |
op_source |
Mladenovska , Z , Mathrani , I M & Ahring , B K 1995 , ' Isolation and characterization of Caldicellulosiruptor lactoaceticus sp. nov., an extremely thermophilic, cellulolytic, anaerobic bacterium ' , Archives of Microbiology , vol. 163 , no. 3 , pp. 223-230 . https://doi.org/10.1007/BF00305357 |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1007/BF00305357 |
container_title |
Archives of Microbiology |
container_volume |
163 |
container_issue |
3 |
container_start_page |
223 |
op_container_end_page |
230 |
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1775352023994073088 |