Quorum sensing in Aeromonas salmonicida subsp. achromogenes and the effect of the autoinducer synthase AsaI on bacterial virulence
The Gram-negative fish pathogenic bacterium Aeromonas salmonicida possesses the LuxIRtype quorum sensing (QS) system, termed AsaIR. In this study the role of QS in A. salmonicida subsp. achromogenes virulence and pigment production was investigated. Five wild-type Asa strains induced the N-acyl-homo...
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2011
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ftdtupubl:oai:pure.atira.dk:publications/0eacd9e8-d36d-4057-ae64-3f0a6f227f4d 2024-06-23T07:48:55+00:00 Quorum sensing in Aeromonas salmonicida subsp. achromogenes and the effect of the autoinducer synthase AsaI on bacterial virulence Schwenteit, Johanna Gram, Lone Nielsen, Kristian Fog Fridjonsson, Olafur H. Bornscheuer, Uwe T. Givskov, Michael Gudmundsdottir, Bjarnheidur K. 2011 https://orbit.dtu.dk/en/publications/0eacd9e8-d36d-4057-ae64-3f0a6f227f4d https://doi.org/10.1016/j.vetmic.2010.07.020 eng eng https://orbit.dtu.dk/en/publications/0eacd9e8-d36d-4057-ae64-3f0a6f227f4d info:eu-repo/semantics/closedAccess Schwenteit , J , Gram , L , Nielsen , K F , Fridjonsson , O H , Bornscheuer , U T , Givskov , M & Gudmundsdottir , B K 2011 , ' Quorum sensing in Aeromonas salmonicida subsp. achromogenes and the effect of the autoinducer synthase AsaI on bacterial virulence ' , Veterinary Microbiology , vol. 147 , no. 3-4 , pp. 389 . https://doi.org/10.1016/j.vetmic.2010.07.020 Virulence AsaI Quorum sensing Aeromonas salmonicida subsp. achromogenes Quorum sensing inhibitors Arctic charr article 2011 ftdtupubl https://doi.org/10.1016/j.vetmic.2010.07.020 2024-06-11T14:29:46Z The Gram-negative fish pathogenic bacterium Aeromonas salmonicida possesses the LuxIRtype quorum sensing (QS) system, termed AsaIR. In this study the role of QS in A. salmonicida subsp. achromogenes virulence and pigment production was investigated. Five wild-type Asa strains induced the N-acyl-homoserinelactone (AHL) monitor bacteria. HPLC–HR-MS analysis identified only one type of AHL, N-butanoyl-L-homoserine lactone (C4-HSL). A knock out mutant of AsaI, constructed by allelic exchange, did not produce a detectable QS signal and its virulence in fish was significantly impaired, as LD50 of the AsaIdeficient mutant was 20-fold higher than that of the isogenic wt strain and the mean day to death of the mutant was significantly prolonged. Furthermore, the expression of two virulence factors (a toxic protease, AsaP1, and a cytotoxic factor) and a brown pigment were reduced in the mutant. AsaP1 productionwas inhibited by synthetic QS inhibitors (N- (propylsulfanylacetyl)-L-homoserine lactone; N-(pentylsulfanylacetyl)-L-homoserine lactone; and N-(heptylsulfanylacetyl)-L-homoserine lactone) at concentrations that did not affect bacterial growth. It is a new finding that the AHL synthase of Aeromonas affects virulence in fish and QS has not previously been associated with A. salmonicida infections in fish. Furthermore, AsaP1 production has not previously been shown to be QS regulated. The simplicity of the A. salmonicida subsp. achromogenes LuxIR-type QS system and the observation that synthetic QSI can inhibit an important virulence factor, AsaP1, without affecting bacterial growth, makes A. salmonicida subsp. achromogenes an interesting target organism to study the effects of QS in disease development and QSI in disease control. Article in Journal/Newspaper Arctic charr Arctic Technical University of Denmark: DTU Orbit Arctic Veterinary Microbiology 147 3-4 389 397 |
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Open Polar |
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Technical University of Denmark: DTU Orbit |
op_collection_id |
ftdtupubl |
language |
English |
topic |
Virulence AsaI Quorum sensing Aeromonas salmonicida subsp. achromogenes Quorum sensing inhibitors Arctic charr |
spellingShingle |
Virulence AsaI Quorum sensing Aeromonas salmonicida subsp. achromogenes Quorum sensing inhibitors Arctic charr Schwenteit, Johanna Gram, Lone Nielsen, Kristian Fog Fridjonsson, Olafur H. Bornscheuer, Uwe T. Givskov, Michael Gudmundsdottir, Bjarnheidur K. Quorum sensing in Aeromonas salmonicida subsp. achromogenes and the effect of the autoinducer synthase AsaI on bacterial virulence |
topic_facet |
Virulence AsaI Quorum sensing Aeromonas salmonicida subsp. achromogenes Quorum sensing inhibitors Arctic charr |
description |
The Gram-negative fish pathogenic bacterium Aeromonas salmonicida possesses the LuxIRtype quorum sensing (QS) system, termed AsaIR. In this study the role of QS in A. salmonicida subsp. achromogenes virulence and pigment production was investigated. Five wild-type Asa strains induced the N-acyl-homoserinelactone (AHL) monitor bacteria. HPLC–HR-MS analysis identified only one type of AHL, N-butanoyl-L-homoserine lactone (C4-HSL). A knock out mutant of AsaI, constructed by allelic exchange, did not produce a detectable QS signal and its virulence in fish was significantly impaired, as LD50 of the AsaIdeficient mutant was 20-fold higher than that of the isogenic wt strain and the mean day to death of the mutant was significantly prolonged. Furthermore, the expression of two virulence factors (a toxic protease, AsaP1, and a cytotoxic factor) and a brown pigment were reduced in the mutant. AsaP1 productionwas inhibited by synthetic QS inhibitors (N- (propylsulfanylacetyl)-L-homoserine lactone; N-(pentylsulfanylacetyl)-L-homoserine lactone; and N-(heptylsulfanylacetyl)-L-homoserine lactone) at concentrations that did not affect bacterial growth. It is a new finding that the AHL synthase of Aeromonas affects virulence in fish and QS has not previously been associated with A. salmonicida infections in fish. Furthermore, AsaP1 production has not previously been shown to be QS regulated. The simplicity of the A. salmonicida subsp. achromogenes LuxIR-type QS system and the observation that synthetic QSI can inhibit an important virulence factor, AsaP1, without affecting bacterial growth, makes A. salmonicida subsp. achromogenes an interesting target organism to study the effects of QS in disease development and QSI in disease control. |
format |
Article in Journal/Newspaper |
author |
Schwenteit, Johanna Gram, Lone Nielsen, Kristian Fog Fridjonsson, Olafur H. Bornscheuer, Uwe T. Givskov, Michael Gudmundsdottir, Bjarnheidur K. |
author_facet |
Schwenteit, Johanna Gram, Lone Nielsen, Kristian Fog Fridjonsson, Olafur H. Bornscheuer, Uwe T. Givskov, Michael Gudmundsdottir, Bjarnheidur K. |
author_sort |
Schwenteit, Johanna |
title |
Quorum sensing in Aeromonas salmonicida subsp. achromogenes and the effect of the autoinducer synthase AsaI on bacterial virulence |
title_short |
Quorum sensing in Aeromonas salmonicida subsp. achromogenes and the effect of the autoinducer synthase AsaI on bacterial virulence |
title_full |
Quorum sensing in Aeromonas salmonicida subsp. achromogenes and the effect of the autoinducer synthase AsaI on bacterial virulence |
title_fullStr |
Quorum sensing in Aeromonas salmonicida subsp. achromogenes and the effect of the autoinducer synthase AsaI on bacterial virulence |
title_full_unstemmed |
Quorum sensing in Aeromonas salmonicida subsp. achromogenes and the effect of the autoinducer synthase AsaI on bacterial virulence |
title_sort |
quorum sensing in aeromonas salmonicida subsp. achromogenes and the effect of the autoinducer synthase asai on bacterial virulence |
publishDate |
2011 |
url |
https://orbit.dtu.dk/en/publications/0eacd9e8-d36d-4057-ae64-3f0a6f227f4d https://doi.org/10.1016/j.vetmic.2010.07.020 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic charr Arctic |
genre_facet |
Arctic charr Arctic |
op_source |
Schwenteit , J , Gram , L , Nielsen , K F , Fridjonsson , O H , Bornscheuer , U T , Givskov , M & Gudmundsdottir , B K 2011 , ' Quorum sensing in Aeromonas salmonicida subsp. achromogenes and the effect of the autoinducer synthase AsaI on bacterial virulence ' , Veterinary Microbiology , vol. 147 , no. 3-4 , pp. 389 . https://doi.org/10.1016/j.vetmic.2010.07.020 |
op_relation |
https://orbit.dtu.dk/en/publications/0eacd9e8-d36d-4057-ae64-3f0a6f227f4d |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1016/j.vetmic.2010.07.020 |
container_title |
Veterinary Microbiology |
container_volume |
147 |
container_issue |
3-4 |
container_start_page |
389 |
op_container_end_page |
397 |
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1802639236841603072 |